This is cutadapt 2.8 with Python 3.8.10 Command line parameters: -j 10 -e 0.2 -m 8 -a AGATCGGAAGAGCACACGTCTGAACTCCAGTCA -A AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT -o /tmp/tmppbo23qmu/adapter_R1_0.fastq.gz -p /tmp/tmppbo23qmu/adapter_R2_0.fastq.gz /dreamworld/ngs_data/exoc/P107_R1.fastq.gz /dreamworld/ngs_data/exoc/P107_R2.fastq.gz Processing reads on 10 cores in paired-end mode ... Finished in 226.14 s (14 us/read; 4.16 M reads/minute). === Summary === Total read pairs processed: 15,675,292 Read 1 with adapter: 964,933 (6.2%) Read 2 with adapter: 58,074 (0.4%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 15,675,292 (100.0%) Total basepairs processed: 5,016,093,440 bp Read 1: 2,351,293,800 bp Read 2: 2,664,799,640 bp Total written (filtered): 5,010,498,616 bp (99.9%) Read 1: 2,346,461,466 bp Read 2: 2,664,037,150 bp === First read: Adapter 1 === Sequence: AGATCGGAAGAGCACACGTCTGAACTCCAGTCA; Type: regular 3'; Length: 33; Trimmed: 964933 times; Reverse-complemented: 0 times No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20-24 bp: 4; 25-29 bp: 5; 30-33 bp: 6 Bases preceding removed adapters: A: 24.2% C: 32.2% G: 30.5% T: 13.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 322802 244926.4 0 322802 4 202476 61231.6 0 66122 136354 5 255187 15307.9 1 21589 233598 6 76347 3827.0 1 8997 67350 7 24826 956.7 1 6723 18103 8 16068 239.2 1 4958 5636 5474 9 15561 59.8 1 4390 1635 9536 10 13005 14.9 2 3340 689 8976 11 8232 3.7 2 2890 347 4995 12 4705 0.9 2 2466 171 1896 172 13 3354 0.2 2 2135 156 603 460 14 2910 0.1 2 1881 136 186 707 15 2279 0.0 3 1584 111 66 518 16 1833 0.0 3 1439 94 36 260 4 17 1482 0.0 3 1211 90 28 140 13 18 1187 0.0 3 1033 94 9 19 32 19 950 0.0 3 816 72 16 13 33 20 870 0.0 4 781 61 9 1 16 2 21 824 0.0 4 708 77 11 3 12 13 22 811 0.0 4 722 67 10 1 8 3 23 723 0.0 4 622 73 14 7 5 2 24 752 0.0 4 639 75 21 4 6 7 25 681 0.0 5 580 76 14 3 4 3 1 26 615 0.0 5 526 66 9 3 3 3 5 27 552 0.0 5 484 51 10 1 1 5 28 529 0.0 5 448 56 9 10 4 1 1 29 402 0.0 5 333 56 7 1 4 1 30 369 0.0 6 320 39 6 3 1 31 386 0.0 6 331 44 8 1 1 1 32 310 0.0 6 247 50 7 5 0 1 33 298 0.0 6 254 36 6 2 34 154 0.0 6 124 20 5 2 1 1 1 35 254 0.0 6 203 40 8 3 36 242 0.0 6 200 27 12 2 1 37 226 0.0 6 186 32 6 0 1 1 38 227 0.0 6 185 33 7 1 1 39 189 0.0 6 156 24 7 0 1 0 1 40 131 0.0 6 105 22 3 0 1 41 151 0.0 6 120 24 5 1 0 0 1 42 116 0.0 6 92 20 4 43 11 0.0 6 8 1 0 2 44 60 0.0 6 46 10 1 3 45 107 0.0 6 82 15 6 3 1 46 89 0.0 6 73 13 3 47 73 0.0 6 56 14 3 48 75 0.0 6 60 9 5 0 1 49 94 0.0 6 84 7 2 1 50 91 0.0 6 74 12 4 1 51 70 0.0 6 54 10 4 2 52 59 0.0 6 45 11 3 53 51 0.0 6 40 8 2 1 54 60 0.0 6 48 10 2 55 47 0.0 6 39 4 3 0 0 1 56 60 0.0 6 53 4 3 57 45 0.0 6 36 7 0 1 1 58 25 0.0 6 18 6 1 59 51 0.0 6 40 5 6 60 41 0.0 6 32 5 4 61 33 0.0 6 23 8 1 0 0 0 1 62 35 0.0 6 29 5 1 63 21 0.0 6 14 5 1 1 64 36 0.0 6 32 2 2 65 22 0.0 6 16 6 66 13 0.0 6 8 2 3 67 14 0.0 6 13 0 1 68 16 0.0 6 13 2 0 0 1 69 12 0.0 6 10 1 1 70 15 0.0 6 14 1 71 24 0.0 6 18 3 2 1 72 13 0.0 6 9 3 0 1 73 21 0.0 6 17 3 1 74 47 0.0 6 31 13 2 0 1 75 10 0.0 6 7 3 76 36 0.0 6 24 9 3 77 27 0.0 6 20 5 1 1 78 10 0.0 6 7 2 1 79 15 0.0 6 7 7 0 1 80 27 0.0 6 17 8 2 81 11 0.0 6 9 1 0 0 1 82 15 0.0 6 12 3 83 9 0.0 6 8 0 0 1 84 2 0.0 6 1 1 86 3 0.0 6 3 87 117 0.0 6 70 34 7 3 2 0 1 88 58 0.0 6 35 18 5 89 19 0.0 6 12 4 3 90 3 0.0 6 2 1 91 14 0.0 6 7 4 1 1 0 1 92 3 0.0 6 0 2 1 93 3 0.0 6 2 1 96 2 0.0 6 2 97 49 0.0 6 45 3 1 98 36 0.0 6 28 6 2 99 10 0.0 6 7 3 100 1 0.0 6 1 104 1 0.0 6 1 110 1 0.0 6 1 124 3 0.0 6 3 125 1 0.0 6 1 === Second read: Adapter 2 === Sequence: AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT; Type: regular 3'; Length: 33; Trimmed: 58074 times; Reverse-complemented: 0 times No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20-24 bp: 4; 25-29 bp: 5; 30-33 bp: 6 Bases preceding removed adapters: A: 37.8% C: 4.8% G: 50.7% T: 6.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 27509 244926.4 0 27509 4 380 61231.6 0 1 379 5 503 15307.9 1 0 503 6 19616 3827.0 1 49 19567 40 23 0.0 6 0 0 0 0 0 1 22 41 12 0.0 6 0 0 0 0 0 0 12 42 753 0.0 6 0 0 0 0 0 31 722 43 701 0.0 6 0 0 0 0 0 659 42 44 746 0.0 6 0 0 0 0 659 73 14 45 681 0.0 6 0 0 0 224 107 38 312 46 618 0.0 6 0 0 1 85 57 433 42 47 553 0.0 6 0 0 0 9 105 40 399 48 529 0.0 6 0 0 1 18 122 21 367 49 406 0.0 6 0 0 0 272 31 74 29 50 371 0.0 6 0 0 263 22 13 5 68 51 388 0.0 6 0 0 333 33 14 2 6 52 310 0.0 6 0 3 0 278 21 3 5 53 301 0.0 6 273 21 1 2 3 0 1 54 153 0.0 6 125 22 4 1 1 55 260 0.0 6 231 17 6 1 1 1 3 56 245 0.0 6 222 18 1 1 2 1 57 227 0.0 6 196 18 1 0 10 1 1 58 228 0.0 6 207 11 4 2 1 3 59 190 0.0 6 172 11 2 2 2 1 60 132 0.0 6 119 9 1 0 1 2 61 152 0.0 6 132 11 3 1 2 2 1 62 115 0.0 6 99 9 2 1 2 1 1 63 10 0.0 6 4 3 2 0 0 0 1 64 60 0.0 6 53 5 1 0 0 1 65 107 0.0 6 91 10 3 0 3 66 89 0.0 6 78 9 0 0 1 1 67 74 0.0 6 65 4 4 0 0 1 68 75 0.0 6 67 6 1 0 1 69 94 0.0 6 89 4 0 0 0 1 70 95 0.0 6 84 4 3 1 3 71 77 0.0 6 61 11 3 1 1 72 60 0.0 6 52 4 2 0 1 0 1 73 50 0.0 6 46 2 1 0 0 0 1 74 60 0.0 6 55 4 0 1 75 46 0.0 6 45 0 0 0 1 76 60 0.0 6 55 3 1 0 1 77 47 0.0 6 41 4 1 0 1 78 28 0.0 6 26 1 0 0 1 79 50 0.0 6 42 6 0 0 1 1 80 43 0.0 6 36 6 0 1 81 32 0.0 6 28 2 0 1 0 1 82 37 0.0 6 32 3 1 1 83 23 0.0 6 16 3 2 1 1 84 39 0.0 6 35 3 0 1 85 22 0.0 6 21 0 0 0 1 86 14 0.0 6 10 4 87 15 0.0 6 13 1 1 88 16 0.0 6 15 1 89 12 0.0 6 12 90 15 0.0 6 14 0 0 0 0 1 91 28 0.0 6 21 6 1 92 16 0.0 6 13 1 1 0 0 0 1 93 23 0.0 6 18 4 1 94 51 0.0 6 35 12 2 2 95 12 0.0 6 8 1 2 1 96 39 0.0 6 29 8 2 97 28 0.0 6 22 4 2 98 10 0.0 6 8 2 99 15 0.0 6 12 3 100 27 0.0 6 24 3 101 11 0.0 6 8 3 102 16 0.0 6 12 4 103 9 0.0 6 7 1 0 0 1 104 2 0.0 6 0 1 0 0 1 106 3 0.0 6 3 107 119 0.0 6 90 22 3 1 2 0 1 108 61 0.0 6 46 12 1 1 1 109 21 0.0 6 18 1 1 0 0 1 110 3 0.0 6 3 111 15 0.0 6 11 3 1 112 3 0.0 6 2 0 0 0 1 113 3 0.0 6 2 1 116 2 0.0 6 2 117 51 0.0 6 38 9 2 2 118 37 0.0 6 31 5 1 119 10 0.0 6 10 120 1 0.0 6 1 124 1 0.0 6 1 130 1 0.0 6 1 144 3 0.0 6 3 145 1 0.0 6 1