This is cutadapt 2.8 with Python 3.8.10 Command line parameters: -j 10 -e 0.2 -m 8 -a AGATCGGAAGAGCACACGTCTGAACTCCAGTCA -A AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT -o /tmp/tmps7vm1adt/adapter_R1_0.fastq.gz -p /tmp/tmps7vm1adt/adapter_R2_0.fastq.gz /dreamworld/ngs_data/20220816_Novogene_data/X201SC22041027-Z01-F003/01.RawData/N28/N28_FKDL220180557-1A_HJCT5DSX3_L1_1.fq.gz /dreamworld/ngs_data/20220816_Novogene_data/X201SC22041027-Z01-F003/01.RawData/N28/N28_FKDL220180557-1A_HJCT5DSX3_L1_2.fq.gz Processing reads on 10 cores in paired-end mode ... Finished in 19.96 s (30 us/read; 1.97 M reads/minute). === Summary === Total read pairs processed: 656,934 Read 1 with adapter: 86,232 (13.1%) Read 2 with adapter: 85,619 (13.0%) Pairs that were too short: 813 (0.1%) Pairs written (passing filters): 656,121 (99.9%) Total basepairs processed: 197,080,200 bp Read 1: 98,540,100 bp Read 2: 98,540,100 bp Total written (filtered): 194,468,032 bp (98.7%) Read 1: 97,234,200 bp Read 2: 97,233,832 bp === First read: Adapter 1 === Sequence: AGATCGGAAGAGCACACGTCTGAACTCCAGTCA; Type: regular 3'; Length: 33; Trimmed: 86232 times; Reverse-complemented: 0 times No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20-24 bp: 4; 25-29 bp: 5; 30-33 bp: 6 Bases preceding removed adapters: A: 24.2% C: 20.5% G: 35.5% T: 18.9% none/other: 0.9% Overview of removed sequences length count expect max.err error counts 3 16394 10264.6 0 16394 4 11632 2566.1 0 5588 6044 5 12642 641.5 1 3117 9525 6 5073 160.4 1 2729 2344 7 3083 40.1 1 2541 542 8 2748 10.0 1 2274 316 158 9 2669 2.5 1 2200 144 325 10 2303 0.6 2 1891 98 314 11 1745 0.2 2 1517 68 160 12 1346 0.0 2 1219 61 63 3 13 1196 0.0 2 1103 58 24 11 14 1165 0.0 2 1083 52 13 17 15 1394 0.0 3 1275 65 19 35 16 1458 0.0 3 1356 85 8 9 17 1357 0.0 3 1253 79 12 13 18 1140 0.0 3 1042 60 21 7 10 19 810 0.0 3 761 34 5 3 7 20 638 0.0 4 579 26 9 8 3 13 21 574 0.0 4 535 16 4 5 3 11 22 493 0.0 4 456 23 7 3 3 1 23 448 0.0 4 406 33 6 1 2 24 465 0.0 4 427 25 5 4 3 1 25 519 0.0 5 453 39 10 7 5 5 26 610 0.0 5 573 20 2 6 3 4 2 27 776 0.0 5 696 44 13 7 4 11 1 28 766 0.0 5 703 43 9 2 4 2 3 29 617 0.0 5 560 33 6 5 8 4 1 30 536 0.0 6 493 28 8 4 0 2 1 31 411 0.0 6 356 37 6 4 4 3 1 32 321 0.0 6 293 25 2 0 0 1 33 326 0.0 6 291 20 4 3 7 1 34 315 0.0 6 279 23 6 5 2 35 334 0.0 6 300 21 6 2 1 2 2 36 307 0.0 6 277 19 7 2 1 1 37 365 0.0 6 335 18 6 4 0 2 38 355 0.0 6 324 18 5 5 1 0 2 39 392 0.0 6 357 25 5 1 1 1 2 40 358 0.0 6 328 15 5 1 5 2 2 41 256 0.0 6 231 17 2 2 2 1 1 42 226 0.0 6 204 15 2 2 1 2 43 184 0.0 6 162 13 6 1 0 0 2 44 233 0.0 6 215 15 1 0 0 2 45 211 0.0 6 193 11 1 2 1 1 2 46 222 0.0 6 208 9 2 0 1 2 47 216 0.0 6 200 11 3 1 1 48 256 0.0 6 239 12 2 1 1 0 1 49 281 0.0 6 250 22 3 0 2 3 1 50 260 0.0 6 239 15 3 1 1 0 1 51 201 0.0 6 186 14 0 0 1 52 198 0.0 6 171 18 3 2 0 1 3 53 146 0.0 6 132 8 2 2 0 2 54 135 0.0 6 124 9 2 55 135 0.0 6 123 9 1 1 0 1 56 155 0.0 6 133 14 2 4 1 1 57 225 0.0 6 210 11 0 2 1 1 58 259 0.0 6 223 23 8 1 0 3 1 59 226 0.0 6 209 12 2 1 0 0 2 60 230 0.0 6 213 9 2 3 0 3 61 145 0.0 6 127 8 6 0 1 2 1 62 132 0.0 6 121 5 3 1 0 1 1 63 143 0.0 6 126 13 1 2 1 64 114 0.0 6 106 2 2 2 0 0 2 65 92 0.0 6 85 5 0 0 0 1 1 66 73 0.0 6 67 5 0 1 67 101 0.0 6 97 3 0 0 0 0 1 68 126 0.0 6 117 4 2 0 1 1 1 69 180 0.0 6 167 9 1 0 0 2 1 70 197 0.0 6 183 11 0 1 2 71 140 0.0 6 127 9 1 1 1 0 1 72 108 0.0 6 95 5 2 2 1 1 2 73 88 0.0 6 83 3 0 2 74 109 0.0 6 102 4 1 0 1 0 1 75 83 0.0 6 77 5 0 0 0 1 76 73 0.0 6 70 3 77 69 0.0 6 66 3 78 58 0.0 6 52 2 0 2 0 0 2 79 63 0.0 6 58 3 1 0 1 80 83 0.0 6 72 6 1 2 1 1 81 91 0.0 6 86 3 1 1 82 71 0.0 6 67 3 0 0 0 1 83 59 0.0 6 54 3 0 1 0 1 84 50 0.0 6 48 1 0 0 1 85 33 0.0 6 30 2 0 0 0 1 86 43 0.0 6 33 9 0 0 0 1 87 39 0.0 6 35 3 1 88 44 0.0 6 37 3 2 1 1 89 58 0.0 6 54 3 0 0 0 0 1 90 39 0.0 6 33 3 0 1 0 0 2 91 54 0.0 6 46 5 0 0 0 1 2 92 31 0.0 6 27 1 3 93 25 0.0 6 24 1 94 23 0.0 6 19 3 0 0 1 95 16 0.0 6 14 1 0 0 1 96 19 0.0 6 16 0 1 0 1 0 1 97 19 0.0 6 19 98 16 0.0 6 16 99 13 0.0 6 11 1 0 1 100 21 0.0 6 19 2 101 15 0.0 6 13 0 0 1 0 0 1 102 19 0.0 6 16 2 1 103 14 0.0 6 13 1 104 15 0.0 6 13 1 1 105 8 0.0 6 6 0 0 0 0 1 1 106 1 0.0 6 1 107 6 0.0 6 5 0 0 0 1 108 8 0.0 6 6 1 0 0 0 0 1 109 5 0.0 6 5 110 4 0.0 6 4 111 4 0.0 6 4 112 3 0.0 6 1 1 0 1 113 7 0.0 6 5 0 1 1 114 5 0.0 6 4 0 1 115 2 0.0 6 2 116 5 0.0 6 5 117 5 0.0 6 5 118 2 0.0 6 2 119 2 0.0 6 1 1 121 2 0.0 6 1 0 0 0 0 0 1 122 1 0.0 6 0 1 123 2 0.0 6 2 125 2 0.0 6 2 127 1 0.0 6 1 132 1 0.0 6 1 136 1 0.0 6 0 0 0 1 137 1 0.0 6 1 138 1 0.0 6 1 143 2 0.0 6 2 144 1 0.0 6 1 150 810 0.0 6 0 774 31 0 2 0 3 === Second read: Adapter 2 === Sequence: AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT; Type: regular 3'; Length: 33; Trimmed: 85619 times; Reverse-complemented: 0 times No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20-24 bp: 4; 25-29 bp: 5; 30-33 bp: 6 Bases preceding removed adapters: A: 24.0% C: 20.6% G: 35.9% T: 19.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 16476 10264.6 0 16476 4 11639 2566.1 0 5748 5891 5 12792 641.5 1 3129 9663 6 5092 160.4 1 2716 2376 7 3132 40.1 1 2541 591 8 2734 10.0 1 2299 262 173 9 2650 2.5 1 2165 176 309 10 2313 0.6 2 1866 112 335 11 1766 0.2 2 1497 93 176 12 1334 0.0 2 1214 67 51 2 13 1175 0.0 2 1089 65 16 5 14 1151 0.0 2 1061 65 15 10 15 1373 0.0 3 1261 71 23 18 16 1454 0.0 3 1306 112 22 14 17 1344 0.0 3 1221 94 20 6 3 18 1129 0.0 3 992 103 24 7 3 19 814 0.0 3 713 66 5 27 3 20 640 0.0 4 537 63 27 8 5 21 574 0.0 4 499 48 4 6 14 3 22 493 0.0 4 415 62 7 4 3 2 23 449 0.0 4 364 67 8 5 3 2 24 455 0.0 4 391 50 11 2 1 25 513 0.0 5 417 65 22 4 1 4 26 612 0.0 5 495 92 14 6 4 1 27 778 0.0 5 600 145 23 4 2 4 28 765 0.0 5 622 109 20 8 2 4 29 612 0.0 5 489 87 16 11 5 4 30 537 0.0 6 446 69 12 8 1 1 31 413 0.0 6 321 66 13 5 2 4 2 32 320 0.0 6 265 48 5 1 1 33 326 0.0 6 274 38 8 2 1 2 1 34 319 0.0 6 256 41 11 3 5 0 3 35 335 0.0 6 265 47 14 3 4 0 2 36 308 0.0 6 259 37 8 3 1 37 359 0.0 6 299 46 10 2 1 0 1 38 352 0.0 6 286 47 10 3 4 1 1 39 389 0.0 6 316 62 8 1 0 2 40 358 0.0 6 297 46 3 2 4 2 4 41 257 0.0 6 216 32 8 1 42 225 0.0 6 192 26 3 2 2 43 181 0.0 6 151 20 2 1 2 2 3 44 236 0.0 6 194 28 7 1 3 1 2 45 212 0.0 6 175 26 4 2 1 1 3 46 219 0.0 6 185 28 3 0 1 1 1 47 218 0.0 6 180 26 5 2 1 2 2 48 253 0.0 6 201 38 5 2 5 0 2 49 279 0.0 6 242 24 6 1 1 3 2 50 255 0.0 6 219 30 3 1 2 51 200 0.0 6 172 22 5 0 0 1 52 197 0.0 6 163 25 6 1 0 1 1 53 148 0.0 6 127 15 1 1 1 1 2 54 134 0.0 6 112 18 1 1 0 1 1 55 136 0.0 6 129 5 0 1 1 56 155 0.0 6 129 18 2 0 5 1 57 226 0.0 6 191 24 6 1 1 2 1 58 259 0.0 6 213 33 8 1 3 1 59 223 0.0 6 193 23 2 0 2 3 60 227 0.0 6 195 23 3 2 2 1 1 61 145 0.0 6 121 13 4 1 1 5 62 132 0.0 6 113 14 3 0 1 0 1 63 142 0.0 6 122 13 4 1 0 1 1 64 115 0.0 6 95 16 1 1 1 1 65 92 0.0 6 78 8 3 1 0 1 1 66 74 0.0 6 67 5 0 0 0 1 1 67 103 0.0 6 89 11 1 0 1 0 1 68 127 0.0 6 103 23 0 0 0 1 69 180 0.0 6 148 28 1 1 0 1 1 70 196 0.0 6 178 12 3 0 0 2 1 71 143 0.0 6 121 14 4 0 2 2 72 104 0.0 6 90 10 1 0 0 2 1 73 90 0.0 6 79 7 2 1 1 74 107 0.0 6 85 18 4 75 83 0.0 6 77 4 0 0 1 0 1 76 73 0.0 6 60 9 1 0 2 0 1 77 69 0.0 6 58 10 1 78 60 0.0 6 49 5 1 1 1 0 3 79 65 0.0 6 48 12 1 1 2 0 1 80 83 0.0 6 70 6 1 1 2 2 1 81 92 0.0 6 77 10 3 0 0 1 1 82 71 0.0 6 69 2 83 56 0.0 6 49 3 2 1 0 1 84 51 0.0 6 47 3 0 0 0 1 85 33 0.0 6 28 3 2 86 44 0.0 6 37 3 0 2 0 1 1 87 39 0.0 6 32 7 88 43 0.0 6 36 3 0 1 0 1 2 89 60 0.0 6 48 6 2 1 1 1 1 90 40 0.0 6 32 3 0 0 0 2 3 91 53 0.0 6 43 4 1 2 0 2 1 92 32 0.0 6 26 3 1 0 1 0 1 93 25 0.0 6 19 3 3 94 23 0.0 6 19 2 0 0 1 1 95 16 0.0 6 14 0 1 0 0 1 96 20 0.0 6 17 2 0 1 97 19 0.0 6 16 2 1 98 16 0.0 6 12 4 99 15 0.0 6 10 3 0 0 0 2 100 21 0.0 6 17 4 101 16 0.0 6 13 0 0 0 0 2 1 102 20 0.0 6 18 0 0 1 0 1 103 16 0.0 6 13 1 1 0 0 0 1 104 15 0.0 6 12 1 0 0 1 1 105 9 0.0 6 5 1 0 1 0 0 2 106 1 0.0 6 1 107 6 0.0 6 5 0 0 0 0 0 1 108 8 0.0 6 5 2 0 0 0 0 1 109 5 0.0 6 3 0 0 1 0 0 1 110 4 0.0 6 4 111 4 0.0 6 3 1 112 3 0.0 6 1 0 0 2 113 7 0.0 6 4 1 1 0 1 114 5 0.0 6 3 1 0 0 0 1 115 2 0.0 6 1 1 116 5 0.0 6 3 2 117 5 0.0 6 4 1 118 2 0.0 6 2 119 2 0.0 6 1 1 121 2 0.0 6 1 0 1 122 1 0.0 6 0 0 0 0 1 123 2 0.0 6 2 124 2 0.0 6 0 0 0 0 1 0 1 125 2 0.0 6 1 1 127 1 0.0 6 1 132 1 0.0 6 1 137 1 0.0 6 1 138 1 0.0 6 1 143 2 0.0 6 2 144 1 0.0 6 1 150 1 0.0 6 0 0 0 0 0 0 1