This is cutadapt 2.8 with Python 3.8.10 Command line parameters: -j 10 -e 0.2 -m 8 -a AGATCGGAAGAGCACACGTCTGAACTCCAGTCA -A AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT -o /tmp/tmpuzw47b8t/adapter_R1_0.fastq.gz -p /tmp/tmpuzw47b8t/adapter_R2_0.fastq.gz /dreamworld/ngs_data/20220816_Novogene_data/X201SC22041027-Z01-F003/01.RawData/N29/N29_FKDL220180557-1A_HJCT5DSX3_L1_1.fq.gz /dreamworld/ngs_data/20220816_Novogene_data/X201SC22041027-Z01-F003/01.RawData/N29/N29_FKDL220180557-1A_HJCT5DSX3_L1_2.fq.gz Processing reads on 10 cores in paired-end mode ... Finished in 7.55 s (11 us/read; 5.55 M reads/minute). === Summary === Total read pairs processed: 698,339 Read 1 with adapter: 137,352 (19.7%) Read 2 with adapter: 135,079 (19.3%) Pairs that were too short: 1,227 (0.2%) Pairs written (passing filters): 697,112 (99.8%) Total basepairs processed: 209,501,700 bp Read 1: 104,750,850 bp Read 2: 104,750,850 bp Total written (filtered): 204,703,146 bp (97.7%) Read 1: 102,344,230 bp Read 2: 102,358,916 bp === First read: Adapter 1 === Sequence: AGATCGGAAGAGCACACGTCTGAACTCCAGTCA; Type: regular 3'; Length: 33; Trimmed: 137352 times; Reverse-complemented: 0 times No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20-24 bp: 4; 25-29 bp: 5; 30-33 bp: 6 Bases preceding removed adapters: A: 26.1% C: 22.8% G: 31.7% T: 18.5% none/other: 0.9% Overview of removed sequences length count expect max.err error counts 3 18192 10911.5 0 18192 4 13630 2727.9 0 7849 5781 5 15305 682.0 1 5559 9746 6 7232 170.5 1 4763 2469 7 5492 42.6 1 4812 680 8 5371 10.7 1 4794 392 185 9 5061 2.7 1 4488 236 337 10 4484 0.7 2 3896 205 383 11 3611 0.2 2 3190 185 236 12 2895 0.0 2 2652 138 98 7 13 2327 0.0 2 2163 122 32 10 14 2436 0.0 2 2266 109 40 21 15 2880 0.0 3 2659 152 37 32 16 3290 0.0 3 3036 188 44 22 17 3100 0.0 3 2846 185 43 25 1 18 2718 0.0 3 2531 137 31 11 8 19 1899 0.0 3 1728 109 26 18 18 20 1579 0.0 4 1439 87 16 9 13 15 21 1325 0.0 4 1196 72 30 8 9 10 22 1200 0.0 4 1073 92 16 10 7 2 23 1092 0.0 4 992 68 14 8 7 3 24 1076 0.0 4 962 91 14 4 2 3 25 1059 0.0 5 977 57 9 6 9 1 26 1492 0.0 5 1354 87 16 11 7 14 3 27 1671 0.0 5 1513 89 24 10 8 17 10 28 1729 0.0 5 1549 121 29 10 12 8 29 1475 0.0 5 1319 102 27 5 13 6 3 30 1178 0.0 6 1058 80 18 10 5 3 4 31 1007 0.0 6 894 70 20 9 7 2 5 32 759 0.0 6 662 71 8 7 5 3 3 33 756 0.0 6 684 59 3 3 2 3 2 34 668 0.0 6 608 37 10 5 3 4 1 35 661 0.0 6 598 49 5 6 0 0 3 36 677 0.0 6 614 47 7 5 1 2 1 37 737 0.0 6 650 56 17 4 5 2 3 38 917 0.0 6 827 69 12 4 3 1 1 39 897 0.0 6 804 66 8 6 9 3 1 40 745 0.0 6 675 53 8 3 1 2 3 41 597 0.0 6 538 44 7 4 2 0 2 42 488 0.0 6 436 35 9 2 1 3 2 43 434 0.0 6 394 22 7 1 3 3 4 44 402 0.0 6 369 16 6 3 5 2 1 45 429 0.0 6 380 40 4 1 1 3 46 512 0.0 6 470 28 10 1 1 0 2 47 496 0.0 6 454 31 1 4 4 1 1 48 575 0.0 6 511 46 10 1 4 2 1 49 504 0.0 6 439 49 8 3 2 2 1 50 485 0.0 6 439 35 7 2 2 51 417 0.0 6 386 18 7 2 1 2 1 52 339 0.0 6 296 31 8 1 1 1 1 53 316 0.0 6 293 21 2 54 270 0.0 6 243 20 5 0 0 2 55 269 0.0 6 246 17 2 2 0 1 1 56 283 0.0 6 256 24 0 0 0 2 1 57 378 0.0 6 353 15 4 3 1 1 1 58 494 0.0 6 443 33 7 5 1 4 1 59 433 0.0 6 386 38 4 3 2 60 395 0.0 6 362 24 4 4 0 0 1 61 288 0.0 6 260 19 3 0 3 1 2 62 242 0.0 6 219 18 0 2 1 1 1 63 224 0.0 6 209 12 2 0 0 1 64 202 0.0 6 190 9 0 1 1 0 1 65 180 0.0 6 166 9 1 1 1 1 1 66 167 0.0 6 148 16 1 0 1 1 67 177 0.0 6 164 7 4 2 68 241 0.0 6 217 19 2 0 0 3 69 303 0.0 6 275 23 2 1 2 70 273 0.0 6 259 10 1 1 1 0 1 71 211 0.0 6 189 15 5 2 72 176 0.0 6 155 13 6 1 1 73 124 0.0 6 112 9 1 0 0 2 74 126 0.0 6 114 9 0 2 1 75 116 0.0 6 105 6 1 2 0 0 2 76 116 0.0 6 105 7 0 2 1 0 1 77 119 0.0 6 107 7 2 0 1 0 2 78 135 0.0 6 121 11 1 0 0 1 1 79 132 0.0 6 117 14 0 0 1 80 134 0.0 6 121 9 2 1 1 81 116 0.0 6 108 5 0 0 1 2 82 99 0.0 6 87 11 0 0 1 83 56 0.0 6 50 4 2 84 76 0.0 6 69 5 1 1 85 73 0.0 6 67 5 1 86 65 0.0 6 57 3 2 0 2 1 87 71 0.0 6 66 5 88 69 0.0 6 64 5 89 72 0.0 6 65 5 0 0 0 1 1 90 70 0.0 6 67 3 91 60 0.0 6 54 6 92 46 0.0 6 43 3 93 34 0.0 6 33 1 94 41 0.0 6 38 1 0 0 1 0 1 95 20 0.0 6 18 2 96 25 0.0 6 22 2 0 1 97 26 0.0 6 20 4 0 1 0 1 98 25 0.0 6 20 4 0 0 0 0 1 99 36 0.0 6 30 4 0 1 1 100 28 0.0 6 24 4 101 27 0.0 6 27 102 11 0.0 6 11 103 18 0.0 6 16 0 1 1 104 4 0.0 6 3 1 105 16 0.0 6 14 0 0 1 1 106 10 0.0 6 8 1 0 0 0 0 1 107 12 0.0 6 10 1 0 0 0 1 108 8 0.0 6 8 109 5 0.0 6 4 0 0 0 1 110 8 0.0 6 8 111 12 0.0 6 10 1 0 0 1 112 10 0.0 6 7 2 0 1 113 10 0.0 6 9 1 114 5 0.0 6 3 0 0 1 0 1 115 7 0.0 6 5 0 1 1 116 3 0.0 6 3 117 1 0.0 6 1 118 2 0.0 6 1 0 0 0 1 119 4 0.0 6 4 120 2 0.0 6 2 121 2 0.0 6 2 122 2 0.0 6 2 123 4 0.0 6 4 124 4 0.0 6 2 1 1 125 1 0.0 6 1 128 3 0.0 6 2 1 131 1 0.0 6 1 134 1 0.0 6 1 140 1 0.0 6 1 143 1 0.0 6 1 146 1 0.0 6 1 150 1223 0.0 6 0 1145 74 3 0 0 1 === Second read: Adapter 2 === Sequence: AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT; Type: regular 3'; Length: 33; Trimmed: 135079 times; Reverse-complemented: 0 times No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20-24 bp: 4; 25-29 bp: 5; 30-33 bp: 6 Bases preceding removed adapters: A: 26.3% C: 23.0% G: 31.8% T: 18.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 18238 10911.5 0 18238 4 13422 2727.9 0 7767 5655 5 15093 682.0 1 5504 9589 6 7083 170.5 1 4733 2350 7 5486 42.6 1 4824 662 8 5365 10.7 1 4827 365 173 9 5064 2.7 1 4434 287 343 10 4443 0.7 2 3794 273 376 11 3563 0.2 2 3155 207 201 12 2883 0.0 2 2592 177 110 4 13 2305 0.0 2 2097 155 44 9 14 2405 0.0 2 2222 122 40 21 15 2855 0.0 3 2619 159 46 31 16 3255 0.0 3 2964 221 46 24 17 3067 0.0 3 2761 239 47 17 3 18 2702 0.0 3 2399 238 38 25 2 19 1886 0.0 3 1644 134 22 71 15 20 1563 0.0 4 1315 151 62 20 15 21 1311 0.0 4 1072 155 26 11 41 6 22 1187 0.0 4 975 151 23 20 5 13 23 1084 0.0 4 916 122 30 11 2 3 24 1053 0.0 4 875 132 27 11 6 1 1 25 1051 0.0 5 867 141 24 8 4 7 26 1478 0.0 5 1184 218 44 19 10 3 27 1670 0.0 5 1321 271 50 14 9 5 28 1719 0.0 5 1333 289 62 19 10 5 1 29 1464 0.0 5 1159 228 42 19 7 8 1 30 1169 0.0 6 920 179 41 15 7 4 3 31 1002 0.0 6 799 140 35 17 6 3 2 32 751 0.0 6 591 116 21 9 7 2 5 33 753 0.0 6 605 105 25 7 3 5 3 34 663 0.0 6 527 102 23 6 2 2 1 35 660 0.0 6 517 111 18 7 4 3 36 676 0.0 6 525 113 25 7 2 1 3 37 738 0.0 6 587 113 25 5 2 1 5 38 910 0.0 6 744 123 20 8 10 3 2 39 892 0.0 6 720 127 21 7 13 1 3 40 738 0.0 6 593 102 22 6 3 5 7 41 594 0.0 6 464 83 26 3 8 3 7 42 486 0.0 6 374 82 17 5 3 2 3 43 429 0.0 6 351 58 12 1 5 1 1 44 394 0.0 6 331 37 14 2 5 4 1 45 428 0.0 6 338 65 5 6 6 5 3 46 503 0.0 6 410 74 12 3 3 0 1 47 499 0.0 6 405 63 14 5 6 3 3 48 568 0.0 6 458 78 13 3 6 5 5 49 498 0.0 6 413 52 13 4 8 6 2 50 483 0.0 6 408 48 12 4 3 6 2 51 413 0.0 6 354 46 5 4 2 2 52 335 0.0 6 266 45 7 1 5 4 7 53 312 0.0 6 269 31 5 3 2 2 54 268 0.0 6 229 26 4 3 2 2 2 55 270 0.0 6 220 32 15 1 0 0 2 56 284 0.0 6 227 43 6 0 5 3 57 374 0.0 6 316 49 2 2 2 2 1 58 492 0.0 6 407 61 14 2 1 4 3 59 431 0.0 6 367 50 8 1 3 1 1 60 395 0.0 6 347 34 4 5 1 3 1 61 280 0.0 6 233 34 8 0 2 2 1 62 238 0.0 6 205 27 4 0 2 63 224 0.0 6 189 25 3 3 1 2 1 64 201 0.0 6 167 28 2 1 1 2 65 177 0.0 6 157 17 2 0 1 66 165 0.0 6 138 20 3 1 1 1 1 67 179 0.0 6 157 16 3 1 1 0 1 68 243 0.0 6 204 32 2 3 1 1 69 300 0.0 6 257 36 2 0 1 1 3 70 274 0.0 6 224 36 10 3 1 71 208 0.0 6 166 35 5 0 1 0 1 72 175 0.0 6 141 25 3 0 2 2 2 73 126 0.0 6 110 12 2 1 0 1 74 125 0.0 6 104 14 3 1 1 0 2 75 115 0.0 6 98 11 1 1 1 3 76 112 0.0 6 95 13 0 2 0 1 1 77 120 0.0 6 105 12 1 1 0 0 1 78 135 0.0 6 118 12 0 1 3 0 1 79 131 0.0 6 114 14 2 0 0 0 1 80 134 0.0 6 115 14 3 1 1 81 116 0.0 6 92 15 3 2 3 1 82 99 0.0 6 84 10 1 1 0 2 1 83 56 0.0 6 45 9 0 0 1 0 1 84 76 0.0 6 68 6 2 85 73 0.0 6 64 6 2 0 0 0 1 86 66 0.0 6 53 8 4 1 87 71 0.0 6 60 8 0 1 0 2 88 69 0.0 6 64 3 2 89 71 0.0 6 63 6 2 90 70 0.0 6 59 7 2 0 0 1 1 91 57 0.0 6 55 2 92 46 0.0 6 41 5 93 34 0.0 6 32 2 94 40 0.0 6 39 0 0 0 0 1 95 21 0.0 6 17 3 0 0 1 96 25 0.0 6 24 1 97 25 0.0 6 17 4 1 1 1 0 1 98 25 0.0 6 18 4 1 1 1 99 37 0.0 6 28 6 2 0 0 0 1 100 29 0.0 6 26 2 0 0 0 1 101 30 0.0 6 22 4 0 1 0 2 1 102 13 0.0 6 12 1 103 18 0.0 6 14 1 0 1 0 1 1 104 5 0.0 6 3 0 0 1 0 0 1 105 16 0.0 6 14 1 0 0 0 0 1 106 9 0.0 6 8 0 0 0 1 107 12 0.0 6 10 1 0 0 0 0 1 108 8 0.0 6 5 2 1 109 6 0.0 6 4 0 0 1 1 110 8 0.0 6 8 111 11 0.0 6 11 112 10 0.0 6 8 1 1 113 11 0.0 6 10 0 0 0 0 0 1 114 6 0.0 6 2 2 0 1 0 0 1 115 7 0.0 6 5 0 1 0 0 1 116 4 0.0 6 3 0 0 0 0 0 1 117 2 0.0 6 1 0 0 0 0 1 118 3 0.0 6 1 2 119 4 0.0 6 3 1 120 2 0.0 6 1 1 121 2 0.0 6 2 122 2 0.0 6 2 123 4 0.0 6 3 1 124 4 0.0 6 2 0 0 1 0 1 125 1 0.0 6 0 0 0 0 0 1 128 3 0.0 6 3 131 1 0.0 6 1 134 1 0.0 6 1 140 1 0.0 6 0 1 143 1 0.0 6 1 146 1 0.0 6 1 150 2 0.0 6 0 1 0 1