This is cutadapt 2.8 with Python 3.8.10 Command line parameters: -j 10 -e 0.2 -m 8 -a AGATCGGAAGAGCACACGTCTGAACTCCAGTCA -A AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT -o /tmp/tmpvog0qgap/adapter_R1_0.fastq.gz -p /tmp/tmpvog0qgap/adapter_R2_0.fastq.gz /dreamworld/ngs_data/20220816_Novogene_data/X201SC22041027-Z01-F003/01.RawData/N32/N32_FKDL220180557-1A_HJCT5DSX3_L1_1.fq.gz /dreamworld/ngs_data/20220816_Novogene_data/X201SC22041027-Z01-F003/01.RawData/N32/N32_FKDL220180557-1A_HJCT5DSX3_L1_2.fq.gz Processing reads on 10 cores in paired-end mode ... Finished in 4.35 s (8 us/read; 7.64 M reads/minute). === Summary === Total read pairs processed: 554,418 Read 1 with adapter: 218,232 (39.4%) Read 2 with adapter: 87,547 (15.8%) Pairs that were too short: 129,584 (23.4%) Pairs written (passing filters): 424,834 (76.6%) Total basepairs processed: 166,325,400 bp Read 1: 83,162,700 bp Read 2: 83,162,700 bp Total written (filtered): 124,245,784 bp (74.7%) Read 1: 62,092,750 bp Read 2: 62,153,034 bp === First read: Adapter 1 === Sequence: AGATCGGAAGAGCACACGTCTGAACTCCAGTCA; Type: regular 3'; Length: 33; Trimmed: 218232 times; Reverse-complemented: 0 times No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20-24 bp: 4; 25-29 bp: 5; 30-33 bp: 6 Bases preceding removed adapters: A: 10.9% C: 9.1% G: 12.8% T: 7.8% none/other: 59.4% Overview of removed sequences length count expect max.err error counts 3 10971 8662.8 0 10971 4 8035 2165.7 0 4571 3464 5 9125 541.4 1 3369 5756 6 4571 135.4 1 3001 1570 7 3461 33.8 1 3029 432 8 3280 8.5 1 2928 257 95 9 3051 2.1 1 2659 187 205 10 2726 0.5 2 2336 139 251 11 2214 0.1 2 1932 141 141 12 1786 0.0 2 1594 125 67 13 1554 0.0 2 1421 97 24 12 14 1603 0.0 2 1475 107 12 9 15 1766 0.0 3 1595 128 27 16 16 2204 0.0 3 1978 189 23 14 17 2018 0.0 3 1806 160 31 17 4 18 1707 0.0 3 1532 129 31 10 5 19 1310 0.0 3 1180 101 10 12 7 20 1140 0.0 4 1022 82 17 8 4 7 21 923 0.0 4 809 80 9 8 4 13 22 844 0.0 4 750 79 5 5 5 23 817 0.0 4 742 63 7 1 4 24 787 0.0 4 692 68 18 2 7 25 759 0.0 5 653 74 16 4 10 2 26 929 0.0 5 826 68 14 6 8 5 2 27 1173 0.0 5 1033 98 14 8 2 13 5 28 1244 0.0 5 1087 115 27 3 8 4 29 1040 0.0 5 918 85 13 6 9 5 4 30 824 0.0 6 720 73 12 13 3 1 2 31 678 0.0 6 592 61 16 5 2 2 32 552 0.0 6 489 45 7 4 3 3 1 33 560 0.0 6 501 42 9 2 1 2 3 34 494 0.0 6 435 43 8 3 2 0 3 35 450 0.0 6 404 25 15 2 1 2 1 36 517 0.0 6 454 47 7 4 1 2 2 37 566 0.0 6 495 52 10 4 2 1 2 38 598 0.0 6 528 49 12 4 2 3 39 632 0.0 6 554 54 6 6 1 4 7 40 534 0.0 6 466 50 8 4 0 3 3 41 464 0.0 6 402 36 11 5 2 1 7 42 381 0.0 6 334 34 7 1 2 0 3 43 346 0.0 6 305 31 1 4 4 0 1 44 330 0.0 6 291 24 5 5 1 0 4 45 306 0.0 6 274 23 3 2 0 0 4 46 310 0.0 6 271 22 4 2 3 6 2 47 407 0.0 6 351 35 6 4 3 1 7 48 406 0.0 6 364 35 4 0 0 3 49 416 0.0 6 376 31 2 0 2 2 3 50 298 0.0 6 265 22 5 2 1 0 3 51 292 0.0 6 264 22 4 0 0 1 1 52 261 0.0 6 228 25 2 3 0 0 3 53 216 0.0 6 191 18 5 0 1 1 54 217 0.0 6 187 19 5 1 0 1 4 55 192 0.0 6 174 13 1 3 0 0 1 56 284 0.0 6 255 19 3 2 1 1 3 57 310 0.0 6 279 23 4 1 0 1 2 58 360 0.0 6 316 29 2 3 2 3 5 59 368 0.0 6 315 34 5 7 2 1 4 60 303 0.0 6 270 22 5 3 1 0 2 61 241 0.0 6 205 29 0 1 1 1 4 62 174 0.0 6 158 9 2 1 2 0 2 63 160 0.0 6 142 11 2 1 1 0 3 64 195 0.0 6 171 13 5 0 1 2 3 65 138 0.0 6 121 9 3 1 2 0 2 66 154 0.0 6 141 12 0 1 67 152 0.0 6 129 18 4 0 0 0 1 68 193 0.0 6 170 12 5 2 1 2 1 69 216 0.0 6 187 22 4 0 2 1 70 206 0.0 6 186 13 2 1 1 1 2 71 194 0.0 6 179 13 0 0 1 1 72 137 0.0 6 122 12 2 0 1 73 117 0.0 6 108 5 1 1 1 0 1 74 112 0.0 6 97 11 2 1 0 0 1 75 105 0.0 6 95 7 1 0 1 1 76 84 0.0 6 78 3 3 77 99 0.0 6 89 4 2 1 0 1 2 78 107 0.0 6 89 11 1 2 0 1 3 79 111 0.0 6 97 12 0 0 1 0 1 80 101 0.0 6 89 9 2 0 1 81 106 0.0 6 93 9 1 0 1 1 1 82 84 0.0 6 75 6 2 1 83 60 0.0 6 49 6 3 0 1 0 1 84 64 0.0 6 56 4 0 1 1 1 1 85 60 0.0 6 53 4 1 0 0 2 86 76 0.0 6 70 4 1 1 87 130 0.0 6 45 81 3 0 0 0 1 88 185 0.0 6 66 108 6 0 4 0 1 89 277 0.0 6 59 208 2 1 6 1 90 103 0.0 6 65 29 6 0 0 1 2 91 125 0.0 6 106 15 2 0 0 0 2 92 37 0.0 6 34 2 1 93 44 0.0 6 36 5 1 0 1 1 94 41 0.0 6 40 1 95 41 0.0 6 32 6 2 0 0 0 1 96 15 0.0 6 14 1 97 27 0.0 6 24 2 0 0 1 98 24 0.0 6 21 2 0 0 0 0 1 99 27 0.0 6 25 2 100 37 0.0 6 31 5 1 101 24 0.0 6 21 2 1 102 18 0.0 6 18 103 17 0.0 6 16 0 0 0 0 1 104 10 0.0 6 9 1 105 9 0.0 6 7 1 0 0 0 0 1 106 14 0.0 6 13 0 1 107 6 0.0 6 6 108 6 0.0 6 6 109 5 0.0 6 2 3 110 7 0.0 6 6 1 111 12 0.0 6 11 1 112 9 0.0 6 7 1 0 0 1 113 11 0.0 6 10 0 1 114 4 0.0 6 3 1 115 6 0.0 6 3 3 116 2 0.0 6 1 0 0 1 117 1 0.0 6 1 118 3 0.0 6 3 119 3 0.0 6 2 0 0 1 120 2 0.0 6 1 1 121 1 0.0 6 0 0 0 0 0 1 122 1 0.0 6 1 123 1 0.0 6 0 0 0 0 0 0 1 127 4 0.0 6 4 128 1 0.0 6 0 1 133 1 0.0 6 1 136 1 0.0 6 1 143 1 0.0 6 1 144 1 0.0 6 1 149 7 0.0 6 0 1 2 2 0 2 150 129575 0.0 6 0 121329 6881 642 165 56 502 === Second read: Adapter 2 === Sequence: AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT; Type: regular 3'; Length: 33; Trimmed: 87547 times; Reverse-complemented: 0 times No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20-24 bp: 4; 25-29 bp: 5; 30-33 bp: 6 Bases preceding removed adapters: A: 27.0% C: 22.4% G: 31.7% T: 18.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 11142 8662.8 0 11142 4 8024 2165.7 0 4614 3410 5 8981 541.4 1 3343 5638 6 4403 135.4 1 3008 1395 7 3418 33.8 1 3033 385 8 3289 8.5 1 2935 220 134 9 3056 2.1 1 2696 149 211 10 2677 0.5 2 2327 137 213 11 2173 0.1 2 1961 107 105 12 1787 0.0 2 1614 98 71 4 13 1525 0.0 2 1414 84 21 6 14 1590 0.0 2 1479 74 30 7 15 1751 0.0 3 1610 80 33 28 16 2181 0.0 3 2016 128 24 13 17 2007 0.0 3 1814 153 19 15 6 18 1691 0.0 3 1506 141 32 10 2 19 1299 0.0 3 1150 81 15 45 8 20 1127 0.0 4 986 88 40 9 4 21 920 0.0 4 782 81 16 14 22 5 22 837 0.0 4 725 95 10 3 3 1 23 815 0.0 4 711 90 9 1 3 1 24 783 0.0 4 680 81 10 5 7 25 750 0.0 5 636 87 10 7 8 2 26 923 0.0 5 770 106 23 15 6 3 27 1158 0.0 5 970 145 23 10 5 5 28 1230 0.0 5 1010 170 33 7 5 5 29 1032 0.0 5 849 147 23 6 4 3 30 821 0.0 6 672 112 21 10 1 2 3 31 675 0.0 6 557 77 19 11 5 3 3 32 550 0.0 6 444 73 13 6 6 3 5 33 558 0.0 6 456 72 15 2 7 5 1 34 483 0.0 6 399 67 9 5 0 3 35 447 0.0 6 360 66 9 2 4 4 2 36 514 0.0 6 442 50 13 4 4 1 37 559 0.0 6 454 77 16 1 4 3 4 38 594 0.0 6 492 80 14 5 3 39 632 0.0 6 530 65 24 4 3 4 2 40 524 0.0 6 440 65 11 1 4 1 2 41 463 0.0 6 383 66 5 2 3 3 1 42 375 0.0 6 320 47 5 2 1 43 344 0.0 6 283 46 9 1 4 0 1 44 327 0.0 6 276 39 2 4 3 2 1 45 302 0.0 6 250 37 9 2 1 3 46 307 0.0 6 255 41 7 1 0 2 1 47 409 0.0 6 327 59 9 4 6 2 2 48 404 0.0 6 346 46 6 2 2 2 49 412 0.0 6 362 41 3 1 1 1 3 50 299 0.0 6 247 38 5 2 5 1 1 51 289 0.0 6 253 28 6 0 2 52 264 0.0 6 217 26 6 4 6 3 2 53 214 0.0 6 185 21 1 3 1 1 2 54 213 0.0 6 184 22 4 1 1 1 55 191 0.0 6 162 23 1 2 2 1 56 285 0.0 6 248 32 3 0 1 1 57 307 0.0 6 259 33 6 2 3 3 1 58 350 0.0 6 289 45 9 2 2 1 2 59 366 0.0 6 317 33 6 4 6 60 303 0.0 6 268 29 2 2 1 1 61 239 0.0 6 217 16 4 0 1 1 62 173 0.0 6 153 17 1 1 0 1 63 157 0.0 6 131 21 3 0 1 0 1 64 190 0.0 6 168 14 3 3 1 0 1 65 141 0.0 6 117 17 2 0 1 2 2 66 157 0.0 6 134 18 2 0 1 2 67 151 0.0 6 129 17 0 1 3 0 1 68 193 0.0 6 172 17 1 0 1 1 1 69 211 0.0 6 183 20 3 1 2 1 1 70 205 0.0 6 177 19 3 1 3 1 1 71 193 0.0 6 176 13 1 2 1 72 136 0.0 6 121 11 2 1 1 73 117 0.0 6 104 9 1 2 1 74 109 0.0 6 92 13 2 1 0 1 75 105 0.0 6 87 12 2 1 1 2 76 83 0.0 6 74 5 2 1 0 1 77 99 0.0 6 83 11 0 4 1 78 107 0.0 6 94 7 2 1 2 1 79 112 0.0 6 99 11 1 0 1 80 99 0.0 6 92 4 1 0 0 2 81 103 0.0 6 92 9 1 1 82 83 0.0 6 72 11 83 60 0.0 6 52 7 1 84 63 0.0 6 51 7 1 0 1 1 2 85 60 0.0 6 53 2 2 2 1 86 75 0.0 6 67 6 1 0 1 87 49 0.0 6 43 4 1 0 1 88 67 0.0 6 60 5 1 0 0 1 89 64 0.0 6 57 4 0 0 1 1 1 90 64 0.0 6 54 7 2 1 91 58 0.0 6 51 3 2 0 0 1 1 92 36 0.0 6 28 3 1 0 1 2 1 93 44 0.0 6 36 5 1 1 1 94 39 0.0 6 36 2 1 95 40 0.0 6 39 1 96 15 0.0 6 14 1 97 26 0.0 6 24 1 0 0 1 98 25 0.0 6 21 0 1 1 0 0 2 99 27 0.0 6 23 3 1 100 36 0.0 6 30 6 101 24 0.0 6 22 1 0 1 102 18 0.0 6 17 0 0 0 0 1 103 17 0.0 6 15 1 0 1 104 11 0.0 6 9 0 1 1 105 10 0.0 6 7 2 0 0 0 1 106 14 0.0 6 9 2 0 0 0 2 1 107 6 0.0 6 4 2 108 6 0.0 6 5 0 0 0 0 1 109 5 0.0 6 4 1 110 7 0.0 6 6 0 0 1 111 12 0.0 6 10 2 112 10 0.0 6 9 0 0 0 0 1 113 5 0.0 6 2 1 1 0 0 1 114 4 0.0 6 3 1 115 5 0.0 6 3 0 0 1 0 1 116 2 0.0 6 1 0 1 117 1 0.0 6 1 118 3 0.0 6 3 119 4 0.0 6 2 0 0 0 0 0 2 120 2 0.0 6 1 1 122 2 0.0 6 1 0 0 0 0 1 123 1 0.0 6 0 0 0 0 0 0 1 127 4 0.0 6 4 128 1 0.0 6 0 1 133 1 0.0 6 1 136 1 0.0 6 0 1 143 1 0.0 6 1 144 1 0.0 6 1 150 8 0.0 6 0 0 3 3 0 1 1