This is cutadapt 2.8 with Python 3.8.10 Command line parameters: -j 10 -e 0.2 -m 8 -a AGATCGGAAGAGCACACGTCTGAACTCCAGTCA -A AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT -o /tmp/tmp67b0p92w/adapter_R1_0.fastq.gz -p /tmp/tmp67b0p92w/adapter_R2_0.fastq.gz /dreamworld/ngs_data/20220816_Novogene_data/X201SC22041027-Z01-F003/01.RawData/N40/N40_FKDL220180557-1A_HJCT5DSX3_L1_1.fq.gz /dreamworld/ngs_data/20220816_Novogene_data/X201SC22041027-Z01-F003/01.RawData/N40/N40_FKDL220180557-1A_HJCT5DSX3_L1_2.fq.gz Processing reads on 10 cores in paired-end mode ... Finished in 6.97 s (10 us/read; 5.74 M reads/minute). === Summary === Total read pairs processed: 666,814 Read 1 with adapter: 122,563 (18.4%) Read 2 with adapter: 120,867 (18.1%) Pairs that were too short: 1,329 (0.2%) Pairs written (passing filters): 665,485 (99.8%) Total basepairs processed: 200,044,200 bp Read 1: 100,022,100 bp Read 2: 100,022,100 bp Total written (filtered): 195,474,089 bp (97.7%) Read 1: 97,731,522 bp Read 2: 97,742,567 bp === First read: Adapter 1 === Sequence: AGATCGGAAGAGCACACGTCTGAACTCCAGTCA; Type: regular 3'; Length: 33; Trimmed: 122563 times; Reverse-complemented: 0 times No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20-24 bp: 4; 25-29 bp: 5; 30-33 bp: 6 Bases preceding removed adapters: A: 25.8% C: 23.2% G: 30.7% T: 19.2% none/other: 1.1% Overview of removed sequences length count expect max.err error counts 3 17141 10419.0 0 17141 4 12741 2604.7 0 6979 5762 5 13751 651.2 1 4656 9095 6 6210 162.8 1 3911 2299 7 4275 40.7 1 3684 591 8 4105 10.2 1 3561 354 190 9 4148 2.5 1 3617 222 309 10 3748 0.6 2 3169 188 391 11 2907 0.2 2 2560 152 195 12 2409 0.0 2 2186 110 108 5 13 2067 0.0 2 1877 141 39 10 14 2123 0.0 2 1946 117 39 21 15 2329 0.0 3 2154 113 30 32 16 2587 0.0 3 2394 138 36 19 17 2543 0.0 3 2349 147 30 17 18 2043 0.0 3 1869 136 25 7 6 19 1582 0.0 3 1463 83 21 7 8 20 1268 0.0 4 1160 74 11 13 5 5 21 1226 0.0 4 1146 50 9 7 7 7 22 1010 0.0 4 924 60 20 4 1 1 23 944 0.0 4 859 63 14 3 5 24 949 0.0 4 862 67 13 3 4 25 1001 0.0 5 910 54 12 10 9 6 26 1180 0.0 5 1073 67 9 11 12 8 27 1416 0.0 5 1272 84 16 11 10 14 9 28 1524 0.0 5 1386 105 15 6 10 2 29 1353 0.0 5 1214 87 15 3 22 9 3 30 1026 0.0 6 939 63 12 5 2 2 3 31 858 0.0 6 782 50 14 8 1 1 2 32 725 0.0 6 628 66 17 3 6 5 33 757 0.0 6 680 58 9 4 3 1 2 34 670 0.0 6 602 49 11 4 2 1 1 35 661 0.0 6 587 52 7 8 3 2 2 36 633 0.0 6 566 45 13 1 2 3 3 37 740 0.0 6 666 47 11 8 4 2 2 38 810 0.0 6 736 55 8 5 2 1 3 39 801 0.0 6 726 52 12 5 3 2 1 40 697 0.0 6 624 51 12 6 3 0 1 41 518 0.0 6 476 28 5 3 1 2 3 42 548 0.0 6 497 34 7 4 1 2 3 43 469 0.0 6 414 42 6 3 0 2 2 44 409 0.0 6 389 14 2 0 3 1 45 427 0.0 6 393 27 5 1 0 0 1 46 427 0.0 6 388 20 8 2 2 3 4 47 515 0.0 6 459 40 8 5 1 0 2 48 552 0.0 6 498 38 9 4 0 1 2 49 534 0.0 6 483 36 5 3 2 2 3 50 479 0.0 6 444 30 0 4 0 1 51 444 0.0 6 403 34 2 2 0 2 1 52 382 0.0 6 347 24 10 1 53 299 0.0 6 264 23 4 3 3 0 2 54 310 0.0 6 285 20 1 0 1 2 1 55 303 0.0 6 274 19 3 1 3 0 3 56 360 0.0 6 319 28 5 4 2 1 1 57 369 0.0 6 331 25 6 3 2 1 1 58 470 0.0 6 431 27 4 4 2 0 2 59 435 0.0 6 393 31 6 2 2 1 60 421 0.0 6 378 33 7 2 1 61 283 0.0 6 260 19 1 2 0 1 62 285 0.0 6 260 19 4 2 63 225 0.0 6 207 14 0 3 1 64 211 0.0 6 193 10 3 2 0 2 1 65 196 0.0 6 179 13 2 2 66 191 0.0 6 178 9 4 67 217 0.0 6 200 11 1 0 3 2 68 225 0.0 6 210 13 0 0 0 0 2 69 284 0.0 6 249 24 3 2 3 2 1 70 292 0.0 6 268 21 2 0 1 71 223 0.0 6 201 10 7 1 1 1 2 72 192 0.0 6 175 14 1 2 73 212 0.0 6 185 18 2 2 3 2 74 147 0.0 6 133 11 2 0 1 75 165 0.0 6 153 7 4 0 1 76 117 0.0 6 98 13 2 1 0 1 2 77 119 0.0 6 108 9 1 0 0 0 1 78 159 0.0 6 151 7 1 79 147 0.0 6 135 6 5 0 1 80 126 0.0 6 111 7 4 2 2 81 119 0.0 6 109 8 1 1 82 136 0.0 6 128 4 1 1 1 1 83 90 0.0 6 84 3 1 0 1 1 84 97 0.0 6 89 4 2 1 0 0 1 85 110 0.0 6 103 5 2 86 65 0.0 6 55 6 4 87 63 0.0 6 58 4 0 0 0 0 1 88 75 0.0 6 68 4 0 2 0 1 89 75 0.0 6 67 4 0 2 1 1 90 81 0.0 6 79 2 91 92 0.0 6 83 7 1 1 92 52 0.0 6 49 1 1 0 0 1 93 43 0.0 6 39 3 0 0 0 1 94 62 0.0 6 56 1 3 0 1 1 95 39 0.0 6 36 3 96 34 0.0 6 32 1 1 97 40 0.0 6 37 1 0 0 1 1 98 31 0.0 6 22 5 0 1 3 99 29 0.0 6 23 3 1 1 1 100 29 0.0 6 27 2 101 23 0.0 6 20 0 1 2 102 26 0.0 6 21 1 0 1 0 3 103 26 0.0 6 24 2 104 21 0.0 6 17 1 0 1 1 0 1 105 18 0.0 6 15 0 1 0 1 1 106 13 0.0 6 12 1 107 12 0.0 6 12 108 8 0.0 6 7 1 109 7 0.0 6 7 110 10 0.0 6 10 111 11 0.0 6 11 112 5 0.0 6 3 0 0 0 1 1 113 4 0.0 6 2 0 1 1 114 5 0.0 6 3 2 115 4 0.0 6 4 116 6 0.0 6 4 0 0 0 0 2 117 2 0.0 6 1 0 0 1 118 1 0.0 6 1 119 5 0.0 6 5 120 1 0.0 6 1 122 3 0.0 6 1 0 1 0 1 123 4 0.0 6 4 124 1 0.0 6 0 0 1 126 3 0.0 6 1 2 127 1 0.0 6 0 1 128 2 0.0 6 1 0 1 130 2 0.0 6 2 131 1 0.0 6 1 133 1 0.0 6 1 137 1 0.0 6 1 145 1 0.0 6 1 150 1328 0.0 6 0 1254 62 5 2 0 5 === Second read: Adapter 2 === Sequence: AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT; Type: regular 3'; Length: 33; Trimmed: 120867 times; Reverse-complemented: 0 times No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20-24 bp: 4; 25-29 bp: 5; 30-33 bp: 6 Bases preceding removed adapters: A: 26.0% C: 23.6% G: 30.8% T: 19.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 17344 10419.0 0 17344 4 12736 2604.7 0 7018 5718 5 13724 651.2 1 4578 9146 6 6154 162.8 1 3916 2238 7 4283 40.7 1 3697 586 8 4020 10.2 1 3551 285 184 9 4150 2.5 1 3577 228 345 10 3724 0.6 2 3149 196 379 11 2867 0.2 2 2555 141 171 12 2384 0.0 2 2168 128 83 5 13 2040 0.0 2 1864 133 38 5 14 2095 0.0 2 1953 103 22 17 15 2309 0.0 3 2121 120 33 35 16 2556 0.0 3 2349 148 39 20 17 2523 0.0 3 2299 171 38 14 1 18 2027 0.0 3 1809 160 40 11 7 19 1568 0.0 3 1382 114 8 52 12 20 1261 0.0 4 1085 104 53 16 3 21 1228 0.0 4 1053 103 21 9 34 8 22 1013 0.0 4 875 109 8 9 6 6 23 944 0.0 4 805 110 18 7 3 1 24 942 0.0 4 820 89 15 9 6 0 3 25 1000 0.0 5 853 111 22 4 4 5 1 26 1168 0.0 5 973 142 31 8 8 6 27 1422 0.0 5 1175 189 35 8 10 5 28 1514 0.0 5 1236 214 36 12 11 3 2 29 1358 0.0 5 1099 194 38 13 5 9 30 1022 0.0 6 840 138 22 9 7 2 4 31 851 0.0 6 713 105 17 7 4 3 2 32 723 0.0 6 605 78 18 7 9 3 3 33 751 0.0 6 618 97 18 8 5 1 4 34 665 0.0 6 546 95 13 4 5 1 1 35 642 0.0 6 518 88 19 8 5 2 2 36 623 0.0 6 522 72 20 3 4 1 1 37 738 0.0 6 604 99 13 7 6 4 5 38 807 0.0 6 672 101 17 3 7 4 3 39 795 0.0 6 661 105 11 4 6 4 4 40 697 0.0 6 598 69 17 2 2 6 3 41 516 0.0 6 434 66 8 0 3 0 5 42 541 0.0 6 446 65 18 3 4 3 2 43 464 0.0 6 385 51 15 1 8 3 1 44 410 0.0 6 334 61 10 3 1 1 45 422 0.0 6 352 50 11 2 4 1 2 46 420 0.0 6 333 59 9 7 8 2 2 47 506 0.0 6 416 62 12 3 7 2 4 48 547 0.0 6 468 61 12 1 1 3 1 49 533 0.0 6 437 74 13 3 3 3 50 480 0.0 6 405 57 8 3 5 0 2 51 435 0.0 6 386 37 7 3 0 1 1 52 389 0.0 6 309 58 10 1 3 3 5 53 298 0.0 6 255 34 4 0 4 1 54 309 0.0 6 268 29 8 0 2 1 1 55 301 0.0 6 243 44 5 1 5 1 2 56 357 0.0 6 298 46 4 2 3 2 2 57 364 0.0 6 310 35 11 3 3 1 1 58 465 0.0 6 403 45 6 1 7 1 2 59 432 0.0 6 359 56 8 5 1 2 1 60 419 0.0 6 351 58 5 1 2 2 61 285 0.0 6 252 28 3 0 2 62 285 0.0 6 237 40 5 0 2 0 1 63 227 0.0 6 196 24 4 2 0 1 64 208 0.0 6 177 23 3 3 0 1 1 65 196 0.0 6 162 27 2 3 1 1 66 194 0.0 6 171 16 3 1 2 0 1 67 217 0.0 6 191 19 2 1 2 2 68 228 0.0 6 198 21 2 1 1 4 1 69 286 0.0 6 239 34 4 2 1 3 3 70 291 0.0 6 257 19 6 0 4 3 2 71 222 0.0 6 187 27 2 0 2 2 2 72 192 0.0 6 169 15 2 2 1 1 2 73 205 0.0 6 169 24 1 3 3 3 2 74 147 0.0 6 134 10 0 0 1 0 2 75 163 0.0 6 139 18 1 0 3 0 2 76 114 0.0 6 94 13 0 1 2 3 1 77 118 0.0 6 107 9 1 0 1 78 160 0.0 6 144 12 2 0 0 1 1 79 145 0.0 6 133 8 2 1 1 80 126 0.0 6 108 10 3 0 1 1 3 81 118 0.0 6 96 18 3 0 1 82 137 0.0 6 113 17 2 2 1 1 1 83 90 0.0 6 80 6 2 0 0 1 1 84 96 0.0 6 81 12 1 0 1 1 85 111 0.0 6 95 11 1 2 0 1 1 86 65 0.0 6 56 5 2 1 0 1 87 65 0.0 6 55 8 1 0 0 0 1 88 73 0.0 6 64 9 89 78 0.0 6 60 15 1 0 1 0 1 90 83 0.0 6 70 10 2 0 1 91 82 0.0 6 74 7 0 1 92 53 0.0 6 42 8 1 0 0 0 2 93 42 0.0 6 37 1 0 1 2 0 1 94 63 0.0 6 58 1 0 2 1 0 1 95 39 0.0 6 36 1 0 1 1 96 33 0.0 6 28 4 0 1 97 43 0.0 6 34 5 0 1 2 1 98 28 0.0 6 27 0 0 0 1 99 29 0.0 6 24 3 0 1 0 1 100 29 0.0 6 26 3 101 21 0.0 6 19 1 0 1 102 24 0.0 6 19 2 1 1 1 103 26 0.0 6 21 4 0 1 104 22 0.0 6 15 5 1 0 1 105 17 0.0 6 13 1 2 1 106 12 0.0 6 10 0 2 107 12 0.0 6 11 1 108 8 0.0 6 5 2 1 109 7 0.0 6 7 110 10 0.0 6 8 2 111 11 0.0 6 11 112 5 0.0 6 1 3 1 113 4 0.0 6 2 1 0 0 1 114 4 0.0 6 1 2 0 1 115 4 0.0 6 3 1 116 6 0.0 6 4 1 0 0 0 0 1 117 2 0.0 6 1 0 1 118 1 0.0 6 0 0 1 119 5 0.0 6 4 1 120 1 0.0 6 1 121 1 0.0 6 0 0 0 0 1 122 2 0.0 6 1 1 123 4 0.0 6 3 1 124 2 0.0 6 0 0 0 0 0 0 2 125 1 0.0 6 0 0 0 1 126 3 0.0 6 3 127 2 0.0 6 1 1 128 1 0.0 6 1 130 2 0.0 6 2 131 1 0.0 6 1 133 2 0.0 6 0 0 1 0 0 1 137 1 0.0 6 1 145 1 0.0 6 1