This is cutadapt 2.8 with Python 3.8.10 Command line parameters: -j 10 -e 0.2 -m 8 -a AGATCGGAAGAGCACACGTCTGAACTCCAGTCA -A AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT -o /tmp/tmpav0qc6wj/adapter_R1_0.fastq.gz -p /tmp/tmpav0qc6wj/adapter_R2_0.fastq.gz /dreamworld/ngs_data/20220816_Novogene_data/X201SC22041027-Z01-F003/01.RawData/N41/N41_FKDL220180557-1A_HJCT5DSX3_L1_1.fq.gz /dreamworld/ngs_data/20220816_Novogene_data/X201SC22041027-Z01-F003/01.RawData/N41/N41_FKDL220180557-1A_HJCT5DSX3_L1_2.fq.gz Processing reads on 10 cores in paired-end mode ... Finished in 5.46 s (11 us/read; 5.39 M reads/minute). === Summary === Total read pairs processed: 491,036 Read 1 with adapter: 77,248 (15.7%) Read 2 with adapter: 76,360 (15.6%) Pairs that were too short: 335 (0.1%) Pairs written (passing filters): 490,701 (99.9%) Total basepairs processed: 147,310,800 bp Read 1: 73,655,400 bp Read 2: 73,655,400 bp Total written (filtered): 144,999,889 bp (98.4%) Read 1: 72,497,678 bp Read 2: 72,502,211 bp === First read: Adapter 1 === Sequence: AGATCGGAAGAGCACACGTCTGAACTCCAGTCA; Type: regular 3'; Length: 33; Trimmed: 77248 times; Reverse-complemented: 0 times No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20-24 bp: 4; 25-29 bp: 5; 30-33 bp: 6 Bases preceding removed adapters: A: 25.1% C: 22.7% G: 32.4% T: 19.4% none/other: 0.4% Overview of removed sequences length count expect max.err error counts 3 12377 7672.4 0 12377 4 9028 1918.1 0 4681 4347 5 10107 479.5 1 3117 6990 6 4390 119.9 1 2625 1765 7 3083 30.0 1 2609 474 8 2882 7.5 1 2540 235 107 9 2706 1.9 1 2303 140 263 10 2302 0.5 2 1940 115 247 11 1900 0.1 2 1652 113 135 12 1487 0.0 2 1319 83 81 4 13 1289 0.0 2 1160 96 22 11 14 1160 0.0 2 1058 60 24 18 15 1415 0.0 3 1280 96 25 14 16 1622 0.0 3 1473 118 17 14 17 1541 0.0 3 1365 134 29 12 1 18 1247 0.0 3 1100 119 14 10 4 19 984 0.0 3 882 71 16 9 6 20 770 0.0 4 670 78 11 4 4 3 21 626 0.0 4 548 49 18 3 1 7 22 576 0.0 4 493 64 12 4 3 23 526 0.0 4 461 52 6 4 3 24 515 0.0 4 444 53 13 4 1 25 550 0.0 5 457 65 13 7 7 1 26 668 0.0 5 562 76 8 6 4 5 7 27 861 0.0 5 735 94 15 9 1 4 3 28 982 0.0 5 826 125 12 8 7 1 3 29 760 0.0 5 627 100 8 6 8 8 3 30 591 0.0 6 488 74 17 9 0 2 1 31 484 0.0 6 393 68 13 4 3 2 1 32 412 0.0 6 351 47 7 6 0 0 1 33 356 0.0 6 293 46 10 2 2 3 34 342 0.0 6 300 31 9 2 35 310 0.0 6 259 41 5 1 3 1 36 332 0.0 6 265 54 9 2 0 2 37 385 0.0 6 319 53 7 4 1 1 38 436 0.0 6 378 48 2 5 0 1 2 39 481 0.0 6 423 49 4 5 40 365 0.0 6 310 34 11 2 4 0 4 41 305 0.0 6 259 39 3 2 1 0 1 42 259 0.0 6 226 27 2 1 2 1 43 250 0.0 6 210 28 4 5 2 1 44 213 0.0 6 188 17 7 1 45 245 0.0 6 209 28 5 2 1 46 191 0.0 6 162 23 3 0 1 1 1 47 217 0.0 6 191 18 5 1 0 2 48 233 0.0 6 191 34 4 1 0 2 1 49 263 0.0 6 223 34 6 50 240 0.0 6 214 21 3 1 0 0 1 51 184 0.0 6 143 31 6 1 0 2 1 52 146 0.0 6 121 13 6 3 2 0 1 53 111 0.0 6 95 13 1 1 0 1 54 128 0.0 6 109 17 1 0 0 0 1 55 108 0.0 6 86 18 0 1 1 1 1 56 153 0.0 6 131 17 2 0 1 1 1 57 144 0.0 6 123 14 5 1 0 1 58 186 0.0 6 152 28 1 1 2 2 59 219 0.0 6 191 22 2 3 1 60 179 0.0 6 160 16 1 2 61 140 0.0 6 129 9 0 2 62 94 0.0 6 88 3 1 1 1 63 93 0.0 6 80 10 2 0 0 0 1 64 83 0.0 6 67 16 65 66 0.0 6 55 10 0 0 0 1 66 71 0.0 6 61 4 4 0 0 1 1 67 89 0.0 6 69 17 1 0 0 0 2 68 115 0.0 6 97 16 1 1 69 117 0.0 6 105 9 1 2 70 122 0.0 6 101 11 7 0 2 0 1 71 81 0.0 6 77 2 0 1 0 0 1 72 82 0.0 6 64 15 2 1 73 47 0.0 6 39 7 0 0 0 0 1 74 47 0.0 6 37 6 3 1 75 54 0.0 6 47 5 0 0 1 1 76 34 0.0 6 32 2 77 49 0.0 6 41 7 1 78 54 0.0 6 49 2 1 2 79 53 0.0 6 45 7 0 1 80 55 0.0 6 46 6 2 0 1 81 53 0.0 6 51 1 0 0 0 0 1 82 53 0.0 6 43 7 3 83 32 0.0 6 25 7 84 18 0.0 6 13 3 0 1 1 85 23 0.0 6 22 1 86 33 0.0 6 27 5 0 1 87 29 0.0 6 24 4 0 0 0 0 1 88 18 0.0 6 14 3 0 1 89 23 0.0 6 18 5 90 30 0.0 6 28 1 1 91 19 0.0 6 17 0 1 0 0 1 92 19 0.0 6 17 1 1 93 13 0.0 6 11 2 94 9 0.0 6 7 1 0 0 0 0 1 95 11 0.0 6 9 2 96 9 0.0 6 5 2 1 0 0 1 97 10 0.0 6 6 2 0 0 1 1 98 11 0.0 6 9 0 0 1 0 0 1 99 13 0.0 6 10 2 1 100 13 0.0 6 12 0 1 101 13 0.0 6 12 1 102 12 0.0 6 10 2 103 8 0.0 6 6 2 104 8 0.0 6 7 0 0 0 1 105 6 0.0 6 4 2 106 6 0.0 6 5 1 107 9 0.0 6 5 1 1 1 1 109 2 0.0 6 1 1 110 9 0.0 6 9 111 5 0.0 6 4 0 1 112 4 0.0 6 3 0 0 1 113 2 0.0 6 1 0 1 114 4 0.0 6 1 1 0 1 0 1 115 1 0.0 6 1 116 4 0.0 6 3 1 117 1 0.0 6 0 0 0 0 1 118 2 0.0 6 2 119 1 0.0 6 1 122 3 0.0 6 3 123 1 0.0 6 1 126 1 0.0 6 1 128 1 0.0 6 1 130 1 0.0 6 1 134 1 0.0 6 1 135 1 0.0 6 1 145 1 0.0 6 1 146 1 0.0 6 1 147 1 0.0 6 1 150 332 0.0 6 0 295 34 2 0 1 === Second read: Adapter 2 === Sequence: AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT; Type: regular 3'; Length: 33; Trimmed: 76360 times; Reverse-complemented: 0 times No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20-24 bp: 4; 25-29 bp: 5; 30-33 bp: 6 Bases preceding removed adapters: A: 25.4% C: 22.4% G: 32.8% T: 19.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 12388 7672.4 0 12388 4 8810 1918.1 0 4686 4124 5 10004 479.5 1 3111 6893 6 4356 119.9 1 2603 1753 7 3030 30.0 1 2609 421 8 2903 7.5 1 2561 198 144 9 2675 1.9 1 2287 159 229 10 2294 0.5 2 1916 122 256 11 1866 0.1 2 1650 102 114 12 1475 0.0 2 1348 62 60 5 13 1281 0.0 2 1176 74 24 7 14 1136 0.0 2 1051 65 14 6 15 1418 0.0 3 1306 63 25 24 16 1611 0.0 3 1477 98 27 9 17 1532 0.0 3 1381 110 20 15 6 18 1243 0.0 3 1092 117 20 10 4 19 982 0.0 3 881 54 10 34 3 20 763 0.0 4 653 61 35 8 6 21 625 0.0 4 531 49 16 4 20 5 22 574 0.0 4 490 66 7 3 3 5 23 528 0.0 4 463 48 10 4 1 2 24 508 0.0 4 422 66 13 5 1 1 25 545 0.0 5 461 62 16 5 1 26 669 0.0 5 573 67 15 9 3 2 27 858 0.0 5 680 144 23 7 3 1 28 973 0.0 5 788 148 21 7 5 3 1 29 759 0.0 5 630 101 16 5 5 1 1 30 591 0.0 6 479 87 14 5 1 4 1 31 488 0.0 6 401 63 13 5 0 2 4 32 409 0.0 6 331 50 19 5 3 1 33 353 0.0 6 295 47 3 2 3 1 2 34 343 0.0 6 268 61 10 2 1 1 35 308 0.0 6 250 44 7 4 1 2 36 333 0.0 6 289 31 8 2 2 1 37 383 0.0 6 315 55 6 2 3 2 38 434 0.0 6 353 63 12 0 1 2 3 39 482 0.0 6 419 45 12 1 2 1 2 40 365 0.0 6 299 48 9 3 2 3 1 41 307 0.0 6 245 42 8 5 3 2 2 42 259 0.0 6 217 30 10 1 1 43 248 0.0 6 204 32 5 3 0 2 2 44 213 0.0 6 182 24 3 2 0 1 1 45 243 0.0 6 198 35 4 2 2 1 1 46 191 0.0 6 158 24 2 0 2 2 3 47 220 0.0 6 192 21 0 2 2 2 1 48 232 0.0 6 198 28 3 0 1 2 49 264 0.0 6 228 26 3 1 2 3 1 50 240 0.0 6 193 34 6 2 3 2 51 180 0.0 6 157 17 3 2 0 1 52 150 0.0 6 124 15 3 0 4 2 2 53 108 0.0 6 88 17 3 54 127 0.0 6 109 15 1 1 1 55 107 0.0 6 93 8 3 0 2 0 1 56 152 0.0 6 132 19 0 0 0 1 57 144 0.0 6 122 16 4 1 0 1 58 186 0.0 6 156 21 4 1 1 2 1 59 218 0.0 6 192 20 4 1 1 60 178 0.0 6 161 16 1 61 141 0.0 6 118 18 1 0 0 1 3 62 95 0.0 6 79 10 3 0 1 1 1 63 92 0.0 6 78 12 1 0 0 0 1 64 83 0.0 6 72 10 1 65 66 0.0 6 54 10 1 1 66 70 0.0 6 57 9 2 0 1 1 67 89 0.0 6 78 7 2 0 2 68 114 0.0 6 94 18 1 0 0 1 69 116 0.0 6 105 8 0 0 2 1 70 123 0.0 6 109 8 2 0 4 71 82 0.0 6 64 14 2 0 1 1 72 81 0.0 6 69 11 0 0 0 1 73 49 0.0 6 39 9 0 0 0 0 1 74 47 0.0 6 40 5 1 0 0 1 75 52 0.0 6 44 5 1 0 1 0 1 76 35 0.0 6 26 5 3 0 0 0 1 77 49 0.0 6 45 4 78 56 0.0 6 48 3 1 2 0 1 1 79 54 0.0 6 51 1 1 0 1 80 56 0.0 6 48 4 3 0 0 1 81 53 0.0 6 46 7 82 53 0.0 6 43 7 1 1 0 1 83 32 0.0 6 28 3 0 0 0 1 84 16 0.0 6 14 2 85 23 0.0 6 22 1 86 33 0.0 6 28 4 1 87 29 0.0 6 25 2 0 1 0 1 88 18 0.0 6 14 3 1 89 23 0.0 6 19 3 0 0 0 1 90 31 0.0 6 24 5 0 0 0 1 1 91 19 0.0 6 16 2 0 0 0 1 92 19 0.0 6 12 6 1 93 14 0.0 6 9 1 2 2 94 9 0.0 6 7 1 0 0 0 1 95 11 0.0 6 9 1 1 96 10 0.0 6 6 2 0 0 1 1 97 11 0.0 6 6 1 0 0 0 1 3 98 11 0.0 6 7 2 0 0 1 0 1 99 13 0.0 6 10 3 100 13 0.0 6 9 4 101 13 0.0 6 10 2 0 1 102 12 0.0 6 10 2 103 8 0.0 6 7 1 104 7 0.0 6 5 2 105 6 0.0 6 4 1 0 0 0 1 106 6 0.0 6 6 107 8 0.0 6 4 1 0 1 1 0 1 109 3 0.0 6 2 0 0 0 1 110 9 0.0 6 9 111 6 0.0 6 3 1 1 0 0 1 112 3 0.0 6 2 0 0 1 113 1 0.0 6 1 114 4 0.0 6 2 0 0 0 0 1 1 115 2 0.0 6 0 2 116 4 0.0 6 3 1 118 2 0.0 6 1 1 119 1 0.0 6 1 122 3 0.0 6 2 1 123 1 0.0 6 1 126 1 0.0 6 1 128 1 0.0 6 0 1 130 1 0.0 6 1 134 1 0.0 6 1 135 1 0.0 6 1 145 1 0.0 6 1 146 1 0.0 6 1 147 1 0.0 6 1