K21 n 0 n 10000 n 20000 n 30000 n 40000 n 50000 n 60000 n 70000 n 80000 n 90000 n 100000 n 110000 n 120000 n 130000 n 140000 n 150000 n 160000 n 170000 n 180000 n 190000 n 200000 n 210000 n 220000 n 230000 n 240000 n 250000 n 260000 n 270000 n 280000 n 290000 n 300000 n 310000 n 320000 n 330000 n 340000 n 350000 n 360000 n 370000 n 380000 n 390000 n 400000 n 410000 n 420000 n 430000 n 440000 n 450000 n 460000 n 470000 n 480000 n 490000 n 500000 n 510000 n 520000 n 530000 n 540000 n 550000 n 560000 n 570000 n 580000 n 590000 n 600000 n 610000 n 620000 n 630000 n 640000 n 650000 n 660000 n 670000 n 680000 n 690000 n 700000 n 710000 n 720000 n 730000 n 740000 n 750000 n 760000 n 770000 n 780000 n 790000 n 800000 n 810000 n 820000 n 830000 n 840000 n 850000 n 860000 n 870000 n 880000 n 890000 n 900000 n 910000 n 920000 n 930000 n 940000 n 950000 n 960000 n 970000 n 980000 n 990000 n 1000000 n 1010000 n 1020000 n 1030000 n 1040000 n 1050000 n 1060000 n 1070000 n 1080000 n 1090000 n 1100000 n 1110000 n 1120000 n 1130000 n 1140000 n 1150000 n 1160000 n 1170000 n 1180000 n 1190000 n 1200000 n 1210000 n 1220000 n 1230000 n 1240000 n 1250000 n 1260000 n 1270000 n 1280000 n 1290000 n 1300000 n 1310000 n 1320000 n 1330000 n 1340000 n 1350000 n 1360000 n 1370000 n 1380000 n 1390000 n 1400000 n 1410000 n 1420000 n 1430000 n 1440000 n 1450000 n 1460000 n 1470000 n 1480000 n 1490000 n 1500000 n 1510000 n 1520000 n 1530000 n 1540000 n 1550000 n 1560000 n 1570000 n 1580000 n 1590000 n 1600000 n 1610000 n 1620000 n 1630000 n 1640000 n 1650000 n 1660000 n 1670000 n 1680000 n 1690000 n 1700000 n 1710000 n 1720000 n 1730000 n 1740000 n 1750000 n 1760000 n 1770000 n 1780000 n 1790000 n 1800000 n 1810000 n 1820000 n 1830000 n 1840000 n 1850000 n 1860000 n 1870000 n 1880000 n 1890000 n 1900000 n 1910000 n 1920000 n 1930000 n 1940000 n 1950000 n 1960000 n 1970000 n 1980000 n 1990000 n 2000000 n 2010000 n 2020000 n 2030000 n 2040000 n 2050000 n 2060000 n 2070000 n 2080000 n 2090000 n 2100000 n 2110000 n 2120000 n 2130000 n 2140000 n 2150000 n 2160000 n 2170000 n 2180000 n 2190000 n 2200000 n 2210000 n 2220000 n 2230000 n 2240000 n 2250000 n 2260000 n 2270000 n 2280000 n 2290000 n 2300000 n 2310000 n 2320000 n 2330000 n 2340000 n 2350000 n 2360000 n 2370000 n 2380000 n 2390000 n 2400000 n 2410000 n 2420000 n 2430000 n 2440000 n 2450000 n 2460000 n 2470000 n 2480000 n 2490000 n 2500000 n 2510000 n 2520000 n 2530000 n 2540000 n 2550000 n 2560000 n 2570000 n 2580000 n 2590000 n 2600000 n 2610000 n 2620000 n 2630000 n 2640000 n 2650000 n 2660000 n 2670000 n 2680000 n 2690000 n 2700000 n 2710000 n 2720000 n 2730000 n 2740000 n 2750000 n 2760000 n 2770000 n 2780000 n 2790000 n 2800000 n 2810000 n 2820000 n 2830000 n 2840000 n 2850000 n 2860000 n 2870000 n 2880000 n 2890000 n 2900000 n 2910000 n 2920000 n 2930000 n 2940000 n 2950000 n 2960000 n 2970000 n 2980000 n 2990000 n 3000000 n 3010000 n 3020000 n 3030000 n 3040000 n 3050000 n 3060000 n 3070000 n 3080000 n 3090000 n 3100000 n 3110000 n 3120000 n 3130000 n 3140000 n 3150000 n 3160000 n 3170000 n 3180000 n 3190000 n 3200000 n 3210000 n 3220000 n 3230000 n 3240000 n 3250000 n 3260000 n 3270000 n 3280000 n 3290000 n 3300000 n 3310000 n 3320000 n 3330000 n 3340000 n 3350000 n 3360000 n 3370000 n 3380000 n 3390000 n 3400000 n 3410000 n 3420000 n 3430000 n 3440000 n 3450000 n 3460000 n 3470000 n 3480000 n 3490000 n 3500000 n 3510000 n 3520000 n 3530000 n 3540000 n 3550000 n 3560000 n 3570000 n 3580000 n 3590000 n 3600000 n 3610000 n 3620000 n 3630000 n 3640000 n 3650000 n 3660000 n 3670000 n 3680000 n 3690000 n 3700000 n 3710000 n 3720000 n 3730000 n 3740000 n 3750000 n 3760000 n 3770000 n 3780000 n 3790000 n 3800000 n 3810000 n 3820000 n 3830000 n 3840000 n 3850000 n 3860000 n 3870000 n 3880000 n 3890000 n 3900000 n 3910000 n 3920000 n 3930000 n 3940000 n 3950000 n 3960000 n 3970000 n 3980000 n 3990000 n 4000000 n 4010000 n 4020000 n 4030000 n 4040000 n 4050000 n 4060000 n 4070000 n 4080000 n 4090000 n 4100000 n 4110000 n 4120000 n 4130000 n 4140000 n 4150000 n 4160000 n 4170000 n 4180000 n 4190000 n 4200000 n 4210000 n 4220000 n 4230000 n 4240000 n 4250000 n 4260000 n 4270000 n 4280000 n 4290000 n 4300000 n 4310000 n 4320000 n 4330000 n 4340000 n 4350000 n 4360000 n 4370000 n 4380000 n 4390000 n 4400000 n 4410000 n 4420000 n 4430000 n 4440000 n 4450000 n 4460000 n 4470000 n 4480000 n 4490000 n 4500000 n 4510000 n 4520000 n 4530000 n 4540000 n 4550000 n 4560000 n 4570000 n 4580000 n 4590000 n 4600000 n 4610000 n 4620000 n 4630000 n 4640000 This is cutadapt 2.3 with Python 3.7.3 Command line parameters: -a TaiI=ACGT -y {name} -o /mnt/scratch/ws/psbelokopytova/202208081122polya_data/4c_plasmid/2022-06-20//cutadapt/K21_pr_plasmid1_empty_cutTaiI.fq /mnt/scratch/ws/psbelokopytova/202208081122polya_data/4c_plasmid/2022-06-20//cutadapt/K21_pr_plasmid1_empty.fq --overlap 4 -e 0 Processing reads on 1 core in single-end mode ... Finished in 5.07 s (10 us/read; 6.07 M reads/minute). === Summary === Total reads processed: 513,081 Reads with adapters: 175,117 (34.1%) Reads written (passing filters): 513,081 (100.0%) Total basepairs processed: 61,369,725 bp Total written (filtered): 54,092,563 bp (88.1%) === Adapter TaiI === Sequence: ACGT; Type: regular 3'; Length: 4; Trimmed: 175117 times. No. of allowed errors: 0-4 bp: 0 Bases preceding removed adapters: A: 63.1% C: 8.2% G: 25.1% T: 3.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 4 607 2004.2 0 607 5 527 2004.2 0 527 6 545 2004.2 0 545 7 577 2004.2 0 577 8 916 2004.2 0 916 9 604 2004.2 0 604 10 452 2004.2 0 452 11 547 2004.2 0 547 12 520 2004.2 0 520 13 504 2004.2 0 504 14 446 2004.2 0 446 15 466 2004.2 0 466 16 818 2004.2 0 818 17 543 2004.2 0 543 18 4558 2004.2 0 4558 19 862 2004.2 0 862 20 699 2004.2 0 699 21 6323 2004.2 0 6323 22 8233 2004.2 0 8233 23 3355 2004.2 0 3355 24 897 2004.2 0 897 25 846 2004.2 0 846 26 744 2004.2 0 744 27 794 2004.2 0 794 28 826 2004.2 0 826 29 564 2004.2 0 564 30 684 2004.2 0 684 31 12499 2004.2 0 12499 32 1607 2004.2 0 1607 33 581 2004.2 0 581 34 533 2004.2 0 533 35 1669 2004.2 0 1669 36 5596 2004.2 0 5596 37 13953 2004.2 0 13953 38 39566 2004.2 0 39566 39 5425 2004.2 0 5425 40 1503 2004.2 0 1503 41 12862 2004.2 0 12862 42 2614 2004.2 0 2614 43 1046 2004.2 0 1046 44 781 2004.2 0 781 45 733 2004.2 0 733 46 1813 2004.2 0 1813 47 2453 2004.2 0 2453 48 640 2004.2 0 640 49 512 2004.2 0 512 50 538 2004.2 0 538 51 424 2004.2 0 424 52 687 2004.2 0 687 53 438 2004.2 0 438 54 376 2004.2 0 376 55 182 2004.2 0 182 56 430 2004.2 0 430 57 424 2004.2 0 424 58 517 2004.2 0 517 59 349 2004.2 0 349 60 413 2004.2 0 413 61 483 2004.2 0 483 62 400 2004.2 0 400 63 536 2004.2 0 536 64 440 2004.2 0 440 65 394 2004.2 0 394 66 383 2004.2 0 383 67 671 2004.2 0 671 68 548 2004.2 0 548 69 607 2004.2 0 607 70 567 2004.2 0 567 71 562 2004.2 0 562 72 928 2004.2 0 928 73 884 2004.2 0 884 74 1137 2004.2 0 1137 75 644 2004.2 0 644 76 541 2004.2 0 541 77 376 2004.2 0 376 78 530 2004.2 0 530 79 401 2004.2 0 401 80 346 2004.2 0 346 81 436 2004.2 0 436 82 369 2004.2 0 369 83 338 2004.2 0 338 84 246 2004.2 0 246 85 267 2004.2 0 267 86 335 2004.2 0 335 87 372 2004.2 0 372 88 268 2004.2 0 268 89 349 2004.2 0 349 90 259 2004.2 0 259 91 278 2004.2 0 278 92 224 2004.2 0 224 93 186 2004.2 0 186 94 193 2004.2 0 193 95 480 2004.2 0 480 96 853 2004.2 0 853 97 6898 2004.2 0 6898 98 253 2004.2 0 253 99 241 2004.2 0 241 100 258 2004.2 0 258 101 100 2004.2 0 100 102 185 2004.2 0 185 103 86 2004.2 0 86 104 91 2004.2 0 91 105 95 2004.2 0 95 106 346 2004.2 0 346 107 121 2004.2 0 121 108 80 2004.2 0 80 109 81 2004.2 0 81 110 89 2004.2 0 89 111 124 2004.2 0 124 112 69 2004.2 0 69 113 102 2004.2 0 102 114 59 2004.2 0 59 115 41 2004.2 0 41 116 77 2004.2 0 77 117 61 2004.2 0 61 118 61 2004.2 0 61 119 53 2004.2 0 53 120 58 2004.2 0 58 121 25 2004.2 0 25 122 11 2004.2 0 11 This is cutadapt 2.3 with Python 3.7.3 Command line parameters: -a TaiI=ACGT -y {name} -o /mnt/scratch/ws/psbelokopytova/202208081122polya_data/4c_plasmid/2022-06-20//cutadapt/K21_pr_plasmid1_cmv_cutTaiI.fq /mnt/scratch/ws/psbelokopytova/202208081122polya_data/4c_plasmid/2022-06-20//cutadapt/K21_pr_plasmid1_cmv.fq --overlap 4 -e 0 Processing reads on 1 core in single-end mode ... Finished in 4.04 s (10 us/read; 6.22 M reads/minute). === Summary === Total reads processed: 418,888 Reads with adapters: 206,748 (49.4%) Reads written (passing filters): 418,888 (100.0%) Total basepairs processed: 50,274,884 bp Total written (filtered): 35,803,578 bp (71.2%) === Adapter TaiI === Sequence: ACGT; Type: regular 3'; Length: 4; Trimmed: 206748 times. No. of allowed errors: 0-4 bp: 0 Bases preceding removed adapters: A: 21.4% C: 12.7% G: 60.5% T: 5.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 4 865 1636.3 0 865 5 1040 1636.3 0 1040 6 630 1636.3 0 630 7 614 1636.3 0 614 8 1019 1636.3 0 1019 9 673 1636.3 0 673 10 674 1636.3 0 674 11 604 1636.3 0 604 12 568 1636.3 0 568 13 644 1636.3 0 644 14 696 1636.3 0 696 15 629 1636.3 0 629 16 606 1636.3 0 606 17 589 1636.3 0 589 18 593 1636.3 0 593 19 580 1636.3 0 580 20 745 1636.3 0 745 21 644 1636.3 0 644 22 919 1636.3 0 919 23 757 1636.3 0 757 24 769 1636.3 0 769 25 767 1636.3 0 767 26 740 1636.3 0 740 27 730 1636.3 0 730 28 709 1636.3 0 709 29 675 1636.3 0 675 30 630 1636.3 0 630 31 805 1636.3 0 805 32 638 1636.3 0 638 33 746 1636.3 0 746 34 649 1636.3 0 649 35 1058 1636.3 0 1058 36 1963 1636.3 0 1963 37 3712 1636.3 0 3712 38 5834 1636.3 0 5834 39 1538 1636.3 0 1538 40 1132 1636.3 0 1132 41 1171 1636.3 0 1171 42 808 1636.3 0 808 43 845 1636.3 0 845 44 1134 1636.3 0 1134 45 1319 1636.3 0 1319 46 2450 1636.3 0 2450 47 3783 1636.3 0 3783 48 3013 1636.3 0 3013 49 994 1636.3 0 994 50 1138 1636.3 0 1138 51 835 1636.3 0 835 52 684 1636.3 0 684 53 798 1636.3 0 798 54 623 1636.3 0 623 55 641 1636.3 0 641 56 696 1636.3 0 696 57 618 1636.3 0 618 58 842 1636.3 0 842 59 882 1636.3 0 882 60 888 1636.3 0 888 61 725 1636.3 0 725 62 694 1636.3 0 694 63 887 1636.3 0 887 64 810 1636.3 0 810 65 986 1636.3 0 986 66 807 1636.3 0 807 67 1531 1636.3 0 1531 68 1097 1636.3 0 1097 69 769 1636.3 0 769 70 1878 1636.3 0 1878 71 1380 1636.3 0 1380 72 2911 1636.3 0 2911 73 3680 1636.3 0 3680 74 4586 1636.3 0 4586 75 2314 1636.3 0 2314 76 1076 1636.3 0 1076 77 723 1636.3 0 723 78 772 1636.3 0 772 79 774 1636.3 0 774 80 802 1636.3 0 802 81 5878 1636.3 0 5878 82 1432 1636.3 0 1432 83 709 1636.3 0 709 84 635 1636.3 0 635 85 2243 1636.3 0 2243 86 30390 1636.3 0 30390 87 45084 1636.3 0 45084 88 10022 1636.3 0 10022 89 4815 1636.3 0 4815 90 2397 1636.3 0 2397 91 1629 1636.3 0 1629 92 965 1636.3 0 965 93 645 1636.3 0 645 94 544 1636.3 0 544 95 1193 1636.3 0 1193 96 578 1636.3 0 578 97 481 1636.3 0 481 98 446 1636.3 0 446 99 464 1636.3 0 464 100 1447 1636.3 0 1447 101 488 1636.3 0 488 102 321 1636.3 0 321 103 274 1636.3 0 274 104 237 1636.3 0 237 105 172 1636.3 0 172 106 211 1636.3 0 211 107 143 1636.3 0 143 108 183 1636.3 0 183 109 160 1636.3 0 160 110 267 1636.3 0 267 111 434 1636.3 0 434 112 150 1636.3 0 150 113 106 1636.3 0 106 114 160 1636.3 0 160 115 176 1636.3 0 176 116 187 1636.3 0 187 117 130 1636.3 0 130 118 127 1636.3 0 127 119 112 1636.3 0 112 120 113 1636.3 0 113 121 39 1636.3 0 39 122 10 1636.3 0 10 123 3 1636.3 0 3 K22 n 0 n 10000 n 20000 n 30000 n 40000 n 50000 n 60000 n 70000 n 80000 n 90000 n 100000 n 110000 n 120000 n 130000 n 140000 n 150000 n 160000 n 170000 n 180000 n 190000 n 200000 n 210000 n 220000 n 230000 n 240000 n 250000 n 260000 n 270000 n 280000 n 290000 n 300000 n 310000 n 320000 n 330000 n 340000 n 350000 n 360000 n 370000 n 380000 n 390000 n 400000 n 410000 n 420000 n 430000 n 440000 n 450000 n 460000 n 470000 n 480000 n 490000 n 500000 n 510000 n 520000 n 530000 n 540000 n 550000 n 560000 n 570000 n 580000 n 590000 n 600000 n 610000 n 620000 n 630000 n 640000 n 650000 n 660000 n 670000 n 680000 n 690000 n 700000 n 710000 n 720000 n 730000 n 740000 n 750000 n 760000 n 770000 n 780000 n 790000 n 800000 n 810000 n 820000 n 830000 n 840000 n 850000 n 860000 n 870000 n 880000 n 890000 n 900000 n 910000 n 920000 n 930000 n 940000 n 950000 n 960000 n 970000 n 980000 n 990000 n 1000000 n 1010000 n 1020000 n 1030000 n 1040000 n 1050000 n 1060000 n 1070000 n 1080000 n 1090000 n 1100000 n 1110000 n 1120000 n 1130000 n 1140000 n 1150000 n 1160000 n 1170000 n 1180000 n 1190000 n 1200000 n 1210000 n 1220000 n 1230000 n 1240000 n 1250000 n 1260000 n 1270000 n 1280000 n 1290000 n 1300000 n 1310000 n 1320000 n 1330000 n 1340000 n 1350000 n 1360000 n 1370000 n 1380000 n 1390000 n 1400000 n 1410000 n 1420000 n 1430000 n 1440000 n 1450000 n 1460000 n 1470000 n 1480000 n 1490000 n 1500000 n 1510000 n 1520000 n 1530000 n 1540000 n 1550000 n 1560000 n 1570000 n 1580000 n 1590000 n 1600000 n 1610000 n 1620000 n 1630000 n 1640000 n 1650000 n 1660000 n 1670000 n 1680000 n 1690000 n 1700000 n 1710000 n 1720000 n 1730000 n 1740000 n 1750000 n 1760000 n 1770000 n 1780000 n 1790000 n 1800000 n 1810000 n 1820000 n 1830000 n 1840000 n 1850000 n 1860000 n 1870000 n 1880000 n 1890000 n 1900000 n 1910000 n 1920000 n 1930000 n 1940000 n 1950000 n 1960000 n 1970000 n 1980000 n 1990000 n 2000000 n 2010000 n 2020000 n 2030000 n 2040000 n 2050000 n 2060000 n 2070000 n 2080000 n 2090000 n 2100000 n 2110000 n 2120000 n 2130000 n 2140000 n 2150000 n 2160000 n 2170000 n 2180000 n 2190000 n 2200000 n 2210000 n 2220000 n 2230000 n 2240000 n 2250000 n 2260000 n 2270000 n 2280000 n 2290000 n 2300000 n 2310000 n 2320000 n 2330000 n 2340000 n 2350000 n 2360000 n 2370000 n 2380000 n 2390000 n 2400000 n 2410000 n 2420000 n 2430000 n 2440000 n 2450000 n 2460000 n 2470000 n 2480000 n 2490000 n 2500000 n 2510000 n 2520000 n 2530000 n 2540000 n 2550000 n 2560000 n 2570000 n 2580000 n 2590000 n 2600000 n 2610000 n 2620000 n 2630000 n 2640000 n 2650000 n 2660000 n 2670000 n 2680000 n 2690000 n 2700000 n 2710000 n 2720000 n 2730000 n 2740000 n 2750000 n 2760000 n 2770000 n 2780000 n 2790000 n 2800000 n 2810000 n 2820000 n 2830000 n 2840000 n 2850000 n 2860000 n 2870000 n 2880000 n 2890000 n 2900000 n 2910000 n 2920000 n 2930000 n 2940000 n 2950000 n 2960000 n 2970000 n 2980000 This is cutadapt 2.3 with Python 3.7.3 Command line parameters: -a TaiI=ACGT -y {name} -o /mnt/scratch/ws/psbelokopytova/202208081122polya_data/4c_plasmid/2022-06-20//cutadapt/K22_pr_plasmid1_empty_cutTaiI.fq /mnt/scratch/ws/psbelokopytova/202208081122polya_data/4c_plasmid/2022-06-20//cutadapt/K22_pr_plasmid1_empty.fq --overlap 4 -e 0 Processing reads on 1 core in single-end mode ... Finished in 2.65 s (9 us/read; 6.49 M reads/minute). === Summary === Total reads processed: 287,046 Reads with adapters: 94,379 (32.9%) Reads written (passing filters): 287,046 (100.0%) Total basepairs processed: 34,318,554 bp Total written (filtered): 30,490,155 bp (88.8%) === Adapter TaiI === Sequence: ACGT; Type: regular 3'; Length: 4; Trimmed: 94379 times. No. of allowed errors: 0-4 bp: 0 Bases preceding removed adapters: A: 59.4% C: 8.8% G: 28.1% T: 3.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 4 366 1121.3 0 366 5 291 1121.3 0 291 6 310 1121.3 0 310 7 341 1121.3 0 341 8 564 1121.3 0 564 9 329 1121.3 0 329 10 313 1121.3 0 313 11 301 1121.3 0 301 12 249 1121.3 0 249 13 301 1121.3 0 301 14 326 1121.3 0 326 15 255 1121.3 0 255 16 605 1121.3 0 605 17 326 1121.3 0 326 18 3610 1121.3 0 3610 19 632 1121.3 0 632 20 376 1121.3 0 376 21 5612 1121.3 0 5612 22 5008 1121.3 0 5008 23 1742 1121.3 0 1742 24 444 1121.3 0 444 25 435 1121.3 0 435 26 442 1121.3 0 442 27 376 1121.3 0 376 28 301 1121.3 0 301 29 326 1121.3 0 326 30 327 1121.3 0 327 31 10595 1121.3 0 10595 32 1289 1121.3 0 1289 33 335 1121.3 0 335 34 278 1121.3 0 278 35 1117 1121.3 0 1117 36 3851 1121.3 0 3851 37 6768 1121.3 0 6768 38 12537 1121.3 0 12537 39 2001 1121.3 0 2001 40 683 1121.3 0 683 41 8320 1121.3 0 8320 42 1286 1121.3 0 1286 43 482 1121.3 0 482 44 452 1121.3 0 452 45 423 1121.3 0 423 46 777 1121.3 0 777 47 1016 1121.3 0 1016 48 320 1121.3 0 320 49 284 1121.3 0 284 50 238 1121.3 0 238 51 244 1121.3 0 244 52 353 1121.3 0 353 53 196 1121.3 0 196 54 170 1121.3 0 170 55 107 1121.3 0 107 56 185 1121.3 0 185 57 252 1121.3 0 252 58 221 1121.3 0 221 59 242 1121.3 0 242 60 202 1121.3 0 202 61 208 1121.3 0 208 62 198 1121.3 0 198 63 228 1121.3 0 228 64 186 1121.3 0 186 65 236 1121.3 0 236 66 225 1121.3 0 225 67 443 1121.3 0 443 68 379 1121.3 0 379 69 280 1121.3 0 280 70 414 1121.3 0 414 71 342 1121.3 0 342 72 616 1121.3 0 616 73 579 1121.3 0 579 74 494 1121.3 0 494 75 312 1121.3 0 312 76 159 1121.3 0 159 77 169 1121.3 0 169 78 257 1121.3 0 257 79 164 1121.3 0 164 80 173 1121.3 0 173 81 180 1121.3 0 180 82 176 1121.3 0 176 83 143 1121.3 0 143 84 130 1121.3 0 130 85 146 1121.3 0 146 86 241 1121.3 0 241 87 189 1121.3 0 189 88 141 1121.3 0 141 89 159 1121.3 0 159 90 130 1121.3 0 130 91 154 1121.3 0 154 92 143 1121.3 0 143 93 94 1121.3 0 94 94 100 1121.3 0 100 95 287 1121.3 0 287 96 495 1121.3 0 495 97 3994 1121.3 0 3994 98 116 1121.3 0 116 99 136 1121.3 0 136 100 242 1121.3 0 242 101 71 1121.3 0 71 102 110 1121.3 0 110 103 71 1121.3 0 71 104 27 1121.3 0 27 105 25 1121.3 0 25 106 264 1121.3 0 264 107 72 1121.3 0 72 108 49 1121.3 0 49 109 35 1121.3 0 35 110 60 1121.3 0 60 111 45 1121.3 0 45 112 28 1121.3 0 28 113 39 1121.3 0 39 114 44 1121.3 0 44 115 23 1121.3 0 23 116 42 1121.3 0 42 117 51 1121.3 0 51 118 47 1121.3 0 47 119 32 1121.3 0 32 120 37 1121.3 0 37 121 13 1121.3 0 13 122 4 1121.3 0 4 This is cutadapt 2.3 with Python 3.7.3 Command line parameters: -a TaiI=ACGT -y {name} -o /mnt/scratch/ws/psbelokopytova/202208081122polya_data/4c_plasmid/2022-06-20//cutadapt/K22_pr_plasmid1_cmv_cutTaiI.fq /mnt/scratch/ws/psbelokopytova/202208081122polya_data/4c_plasmid/2022-06-20//cutadapt/K22_pr_plasmid1_cmv.fq --overlap 4 -e 0 Processing reads on 1 core in single-end mode ... Finished in 3.18 s (10 us/read; 6.22 M reads/minute). === Summary === Total reads processed: 329,800 Reads with adapters: 145,497 (44.1%) Reads written (passing filters): 329,800 (100.0%) Total basepairs processed: 39,538,490 bp Total written (filtered): 29,961,505 bp (75.8%) === Adapter TaiI === Sequence: ACGT; Type: regular 3'; Length: 4; Trimmed: 145497 times. No. of allowed errors: 0-4 bp: 0 Bases preceding removed adapters: A: 25.5% C: 15.8% G: 51.7% T: 6.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 4 692 1288.3 0 692 5 909 1288.3 0 909 6 460 1288.3 0 460 7 479 1288.3 0 479 8 937 1288.3 0 937 9 540 1288.3 0 540 10 495 1288.3 0 495 11 514 1288.3 0 514 12 487 1288.3 0 487 13 490 1288.3 0 490 14 491 1288.3 0 491 15 453 1288.3 0 453 16 475 1288.3 0 475 17 538 1288.3 0 538 18 524 1288.3 0 524 19 478 1288.3 0 478 20 593 1288.3 0 593 21 531 1288.3 0 531 22 765 1288.3 0 765 23 667 1288.3 0 667 24 579 1288.3 0 579 25 544 1288.3 0 544 26 587 1288.3 0 587 27 535 1288.3 0 535 28 525 1288.3 0 525 29 485 1288.3 0 485 30 584 1288.3 0 584 31 1055 1288.3 0 1055 32 675 1288.3 0 675 33 590 1288.3 0 590 34 496 1288.3 0 496 35 1179 1288.3 0 1179 36 2287 1288.3 0 2287 37 3696 1288.3 0 3696 38 4179 1288.3 0 4179 39 1241 1288.3 0 1241 40 1066 1288.3 0 1066 41 1253 1288.3 0 1253 42 801 1288.3 0 801 43 699 1288.3 0 699 44 1458 1288.3 0 1458 45 1210 1288.3 0 1210 46 2634 1288.3 0 2634 47 3942 1288.3 0 3942 48 2805 1288.3 0 2805 49 781 1288.3 0 781 50 766 1288.3 0 766 51 598 1288.3 0 598 52 516 1288.3 0 516 53 523 1288.3 0 523 54 422 1288.3 0 422 55 548 1288.3 0 548 56 500 1288.3 0 500 57 520 1288.3 0 520 58 685 1288.3 0 685 59 708 1288.3 0 708 60 682 1288.3 0 682 61 652 1288.3 0 652 62 633 1288.3 0 633 63 833 1288.3 0 833 64 580 1288.3 0 580 65 853 1288.3 0 853 66 637 1288.3 0 637 67 1650 1288.3 0 1650 68 1102 1288.3 0 1102 69 648 1288.3 0 648 70 1518 1288.3 0 1518 71 1106 1288.3 0 1106 72 3071 1288.3 0 3071 73 3001 1288.3 0 3001 74 2871 1288.3 0 2871 75 1568 1288.3 0 1568 76 727 1288.3 0 727 77 548 1288.3 0 548 78 519 1288.3 0 519 79 576 1288.3 0 576 80 631 1288.3 0 631 81 11525 1288.3 0 11525 82 1621 1288.3 0 1621 83 642 1288.3 0 642 84 484 1288.3 0 484 85 1677 1288.3 0 1677 86 16154 1288.3 0 16154 87 18218 1288.3 0 18218 88 3939 1288.3 0 3939 89 2110 1288.3 0 2110 90 997 1288.3 0 997 91 844 1288.3 0 844 92 621 1288.3 0 621 93 435 1288.3 0 435 94 430 1288.3 0 430 95 1291 1288.3 0 1291 96 427 1288.3 0 427 97 319 1288.3 0 319 98 336 1288.3 0 336 99 405 1288.3 0 405 100 1265 1288.3 0 1265 101 377 1288.3 0 377 102 284 1288.3 0 284 103 249 1288.3 0 249 104 173 1288.3 0 173 105 157 1288.3 0 157 106 295 1288.3 0 295 107 171 1288.3 0 171 108 177 1288.3 0 177 109 143 1288.3 0 143 110 261 1288.3 0 261 111 357 1288.3 0 357 112 180 1288.3 0 180 113 125 1288.3 0 125 114 178 1288.3 0 178 115 343 1288.3 0 343 116 184 1288.3 0 184 117 185 1288.3 0 185 118 126 1288.3 0 126 119 79 1288.3 0 79 120 81 1288.3 0 81 121 25 1288.3 0 25 122 8 1288.3 0 8 123 3 1288.3 0 3