C2 n 0 n 10000 n 20000 n 30000 n 40000 n 50000 n 60000 n 70000 n 80000 n 90000 n 100000 n 110000 n 120000 n 130000 n 140000 n 150000 n 160000 n 170000 n 180000 n 190000 n 200000 n 210000 n 220000 n 230000 n 240000 n 250000 n 260000 n 270000 n 280000 n 290000 n 300000 n 310000 n 320000 n 330000 n 340000 n 350000 n 360000 n 370000 n 380000 n 390000 n 400000 n 410000 n 420000 n 430000 n 440000 n 450000 n 460000 n 470000 n 480000 n 490000 n 500000 n 510000 n 520000 n 530000 n 540000 n 550000 n 560000 n 570000 n 580000 n 590000 n 600000 n 610000 n 620000 n 630000 n 640000 n 650000 n 660000 n 670000 n 680000 n 690000 n 700000 n 710000 n 720000 n 730000 n 740000 n 750000 n 760000 n 770000 n 780000 n 790000 n 800000 n 810000 n 820000 n 830000 n 840000 n 850000 n 860000 n 870000 n 880000 n 890000 n 900000 n 910000 n 920000 n 930000 n 940000 n 950000 n 960000 n 970000 n 980000 n 990000 n 1000000 n 1010000 n 1020000 n 1030000 n 1040000 n 1050000 n 1060000 n 1070000 n 1080000 n 1090000 n 1100000 n 1110000 n 1120000 n 1130000 n 1140000 n 1150000 n 1160000 n 1170000 n 1180000 n 1190000 n 1200000 n 1210000 n 1220000 n 1230000 n 1240000 n 1250000 n 1260000 n 1270000 n 1280000 n 1290000 n 1300000 n 1310000 n 1320000 n 1330000 n 1340000 n 1350000 n 1360000 n 1370000 n 1380000 n 1390000 n 1400000 n 1410000 n 1420000 n 1430000 n 1440000 n 1450000 n 1460000 n 1470000 n 1480000 n 1490000 n 1500000 n 1510000 n 1520000 n 1530000 n 1540000 n 1550000 n 1560000 n 1570000 n 1580000 n 1590000 n 1600000 n 1610000 n 1620000 n 1630000 n 1640000 n 1650000 n 1660000 n 1670000 n 1680000 n 1690000 n 1700000 n 1710000 n 1720000 n 1730000 n 1740000 n 1750000 n 1760000 n 1770000 n 1780000 n 1790000 n 1800000 n 1810000 n 1820000 n 1830000 n 1840000 n 1850000 n 1860000 n 1870000 n 1880000 n 1890000 n 1900000 n 1910000 n 1920000 n 1930000 n 1940000 n 1950000 n 1960000 n 1970000 n 1980000 n 1990000 n 2000000 n 2010000 n 2020000 n 2030000 n 2040000 n 2050000 n 2060000 n 2070000 n 2080000 n 2090000 n 2100000 n 2110000 n 2120000 n 2130000 n 2140000 n 2150000 n 2160000 n 2170000 n 2180000 n 2190000 n 2200000 n 2210000 n 2220000 n 2230000 n 2240000 n 2250000 n 2260000 n 2270000 n 2280000 n 2290000 n 2300000 n 2310000 n 2320000 n 2330000 n 2340000 n 2350000 n 2360000 n 2370000 n 2380000 n 2390000 n 2400000 n 2410000 n 2420000 n 2430000 n 2440000 n 2450000 n 2460000 n 2470000 n 2480000 n 2490000 n 2500000 n 2510000 n 2520000 n 2530000 n 2540000 n 2550000 n 2560000 n 2570000 n 2580000 n 2590000 n 2600000 n 2610000 n 2620000 n 2630000 n 2640000 n 2650000 n 2660000 n 2670000 n 2680000 n 2690000 n 2700000 n 2710000 n 2720000 n 2730000 n 2740000 n 2750000 n 2760000 n 2770000 n 2780000 n 2790000 n 2800000 n 2810000 n 2820000 n 2830000 n 2840000 n 2850000 n 2860000 n 2870000 n 2880000 n 2890000 n 2900000 n 2910000 n 2920000 n 2930000 n 2940000 n 2950000 n 2960000 n 2970000 n 2980000 n 2990000 n 3000000 n 3010000 n 3020000 n 3030000 n 3040000 n 3050000 n 3060000 n 3070000 n 3080000 n 3090000 n 3100000 n 3110000 n 3120000 n 3130000 n 3140000 n 3150000 n 3160000 n 3170000 n 3180000 n 3190000 n 3200000 n 3210000 n 3220000 n 3230000 n 3240000 n 3250000 n 3260000 n 3270000 This is cutadapt 2.3 with Python 3.7.3 Command line parameters: -a TaiI=ACGT -y {name} -o /mnt/scratch/ws/psbelokopytova/202208081122polya_data/4c_plasmid/2022-06-20//cutadapt/C2_pr_plasmid1_cutTaiI.fq /mnt/scratch/ws/psbelokopytova/202208081122polya_data/4c_plasmid/2022-06-20//cutadapt/C2_pr_plasmid1.fq --overlap 4 -e 0 Processing reads on 1 core in single-end mode ... Finished in 7.14 s (10 us/read; 6.11 M reads/minute). === Summary === Total reads processed: 727,874 Reads with adapters: 430,260 (59.1%) Reads written (passing filters): 727,874 (100.0%) Total basepairs processed: 87,300,069 bp Total written (filtered): 62,451,716 bp (71.5%) === Adapter TaiI === Sequence: ACGT; Type: regular 3'; Length: 4; Trimmed: 430260 times. No. of allowed errors: 0-4 bp: 0 Bases preceding removed adapters: A: 31.4% C: 2.2% G: 59.0% T: 7.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 4 572 2843.3 0 572 5 871 2843.3 0 871 6 317 2843.3 0 317 7 244 2843.3 0 244 8 865 2843.3 0 865 9 384 2843.3 0 384 10 296 2843.3 0 296 11 282 2843.3 0 282 12 268 2843.3 0 268 13 239 2843.3 0 239 14 362 2843.3 0 362 15 252 2843.3 0 252 16 310 2843.3 0 310 17 502 2843.3 0 502 18 16051 2843.3 0 16051 19 1795 2843.3 0 1795 20 608 2843.3 0 608 21 13053 2843.3 0 13053 22 5336 2843.3 0 5336 23 862 2843.3 0 862 24 757 2843.3 0 757 25 378 2843.3 0 378 26 311 2843.3 0 311 27 286 2843.3 0 286 28 1286 2843.3 0 1286 29 387 2843.3 0 387 30 827 2843.3 0 827 31 61263 2843.3 0 61263 32 5625 2843.3 0 5625 33 725 2843.3 0 725 34 293 2843.3 0 293 35 424 2843.3 0 424 36 415 2843.3 0 415 37 434 2843.3 0 434 38 366 2843.3 0 366 39 401 2843.3 0 401 40 1255 2843.3 0 1255 41 111560 2843.3 0 111560 42 10460 2843.3 0 10460 43 1003 2843.3 0 1003 44 1087 2843.3 0 1087 45 780 2843.3 0 780 46 961 2843.3 0 961 47 534 2843.3 0 534 48 323 2843.3 0 323 49 345 2843.3 0 345 50 442 2843.3 0 442 51 326 2843.3 0 326 52 376 2843.3 0 376 53 265 2843.3 0 265 54 250 2843.3 0 250 55 462 2843.3 0 462 56 373 2843.3 0 373 57 264 2843.3 0 264 58 418 2843.3 0 418 59 361 2843.3 0 361 60 289 2843.3 0 289 61 328 2843.3 0 328 62 295 2843.3 0 295 63 360 2843.3 0 360 64 390 2843.3 0 390 65 485 2843.3 0 485 66 419 2843.3 0 419 67 299 2843.3 0 299 68 272 2843.3 0 272 69 479 2843.3 0 479 70 865 2843.3 0 865 71 392 2843.3 0 392 72 301 2843.3 0 301 73 475 2843.3 0 475 74 407 2843.3 0 407 75 449 2843.3 0 449 76 281 2843.3 0 281 77 308 2843.3 0 308 78 436 2843.3 0 436 79 323 2843.3 0 323 80 853 2843.3 0 853 81 72137 2843.3 0 72137 82 6124 2843.3 0 6124 83 738 2843.3 0 738 84 338 2843.3 0 338 85 471 2843.3 0 471 86 22737 2843.3 0 22737 87 2364 2843.3 0 2364 88 417 2843.3 0 417 89 273 2843.3 0 273 90 391 2843.3 0 391 91 519 2843.3 0 519 92 363 2843.3 0 363 93 206 2843.3 0 206 94 206 2843.3 0 206 95 167 2843.3 0 167 96 153 2843.3 0 153 97 221 2843.3 0 221 98 131 2843.3 0 131 99 133 2843.3 0 133 100 137 2843.3 0 137 101 493 2843.3 0 493 102 18227 2843.3 0 18227 103 1801 2843.3 0 1801 104 188 2843.3 0 188 105 385 2843.3 0 385 106 37703 2843.3 0 37703 107 3391 2843.3 0 3391 108 300 2843.3 0 300 109 88 2843.3 0 88 110 116 2843.3 0 116 111 121 2843.3 0 121 112 164 2843.3 0 164 113 161 2843.3 0 161 114 100 2843.3 0 100 115 87 2843.3 0 87 116 163 2843.3 0 163 117 73 2843.3 0 73 118 61 2843.3 0 61 119 79 2843.3 0 79 120 110 2843.3 0 110 121 20 2843.3 0 20 122 2 2843.3 0 2 123 3 2843.3 0 3