FastQCFastQC Report
Mon 27 Mar 2023
CS_(100_ng_lig)_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCS_(100_ng_lig)_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186282
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAAGTACTGATC63373.401831631612287No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTTGGCCAGGCTG9030.48474892904306377No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAAAAAAAAAAA5500.295251285685144No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTAGGAGAACAT4850.2603579519223543No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTTGGTCAGGCTG4250.22814872075670223No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAATGCGCCGCT3460.18573989972192698No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAAATCAATCTA3060.1642670789448256No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAAAGCAAGTAA2990.16050933530883285No Hit
GGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAA2950.1583620532311227No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAAGGAGAACAT2830.1519202069979923No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTCCTGTAATCCCA2670.14333107868715173No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAACCTTTCTTT2460.1320578477791735No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAATTAGATCCC2450.131521027259746No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAATAGTAATCA2340.1256160015460431No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAAACTCTGTGA2020.10843774492436198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)