FastQCFastQC Report
Mon 27 Mar 2023
CS_(100_ng_lig)_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCS_(100_ng_lig)_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186282
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTCTCCTCATGGTCAGATGTCTAGAGGCCGCGTTGCTGGCGTTTTTC83484.481377696181059No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8960.480991185407071No Hit
ATGTTCTCCTAGACTGAGACGAAGCTTCGTCTCTTGCAACCATAGTCA4860.26089477244178183No Hit
ATGTTCTCCTGACGAGCTGTACAAGTAAAGCGGCCGCGACTCTAGATCAT3590.1927185664744849No Hit
ATGTTCTCCTAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAA3460.18573989972192698No Hit
ATGTTCTCCTCTGGAGTTCTTCGCCCACCCTAGGGGGAGGCTAACTGAAA3200.17178256621681107No Hit
ATGTTCTCCTCTTTGCATACTTCTGCCTGCTGGGGAGCCTGGGGACTTTC3050.16373025842539804No Hit
ATGTTCTCCTTAAGACTGAGACGAAGCTTCGTCTCTTGCAACCATAGTCA2840.15245702751741982No Hit
ATGTTCTCCTGTCAGATGTCTAGAGGCCGCGTTGCTGGCGTTTTTCCATA2540.1363524119345938No Hit
CTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTAAGGGATTT2520.13527877089573873No Hit
ATGTTCTCCTCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGG2490.1336683093374561No Hit
ATGTTCTCCTTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGA2380.12776328362375322No Hit
ATGTTCTCCTGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTA2350.12615282206547063No Hit
ATGTTCTCCTCTCGCCTTGAGCCTGGCGAACAGTTCGGCTGGCGCGAGCC2270.12185825791005037No Hit
ATGTTCTCCTGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGA1990.1068272833660794No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)