FastQCFastQC Report
Mon 27 Mar 2023
CS_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCS_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153665
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAAGTACTGATC71004.620440568769727No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTAGGAGAACAT11030.7177952038525364No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTTGGCCAGGCTG6690.4353626395080207No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAAGGAGAACAT6130.39891972797969605No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAATGCGCCGCT4160.27071877135326844No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAAAAAAAAAAA4110.2674649399668109No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAAAGCAAGTAA3350.2180067028926561No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTTGGTCAGGCTG2930.19067451924641265No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAAATCAATCTA2790.1815637913643315No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2150.1399147496176748No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTCCTGTAATCCCA1900.12364559268538704No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAATAGTAATCA1770.1151856310805974No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAATTAGATCCC1770.1151856310805974No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAAGAAAATGTG1590.1034718380893502No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAAAAAGTCAGG1550.10086877298018417No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)