FastQCFastQC Report
Mon 27 Mar 2023
CS_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCS_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153665
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTCTCCTCATGGTCAGATGTCTAGAGGCCGCGTTGCTGGCGTTTTTC66834.349071031139166No Hit
ATGTTCTCCTAGACTGAGACGAAGCTTCGTCTCTTGCAACCATAGTCA11070.7203982689617024No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9660.6286402238635994No Hit
ATGTTCTCCTTAAGACTGAGACGAAGCTTCGTCTCTTGCAACCATAGTCA6200.40347509192073666No Hit
ATGTTCTCCTGACGAGCTGTACAAGTAAAGCGGCCGCGACTCTAGATCAT3560.2316727947157778No Hit
ATGTTCTCCTAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAA3330.2167051703380731No Hit
ATGTTCTCCTCTTTGCATACTTCTGCCTGCTGGGGAGCCTGGGGACTTTC2640.17180229720495885No Hit
ATGTTCTCCTCTGGAGTTCTTCGCCCACCCTAGGGGGAGGCTAACTGAAA2580.16789769954120978No Hit
ATGTTCTCCTGTCAGATGTCTAGAGGCCGCGTTGCTGGCGTTTTTCCATA2320.1509777763316305No Hit
ATGTTCTCCTGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTA2050.1334070868447597No Hit
ATGTTCTCCTCTCGCCTTGAGCCTGGCGAACAGTTCGGCTGGCGCGAGCC1950.1268994240718446No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1830.11909022874434647No Hit
ATGTTCTCCTCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGG1630.10607490319851626No Hit
ATGTTCTCCTTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGA1630.10607490319851626No Hit
ATGTTCTCCTGAAACAGCTATGACCAAGATCAGTACTTAAGACTGAGACG1610.10477337064393323No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)