FastQCFastQC Report
Mon 27 Mar 2023
new_CS_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamenew_CS_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171578
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAAGTACTGATC27701.614426091923207No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTAGGAGAACAT15110.8806490342584714No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAAGGAGAACAT8620.5023954119992073No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTTGGCCAGGCTG7330.4272109477905093No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAATGCGCCGCT7120.414971616407698No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAAAGCAAGTAA4840.2820874471086037No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAAAAAAAAAAA4830.2815046218046603No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAAATCAATCTA4180.24362097704833954No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAACCTTTCTTT3320.19349800090920746No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAATAGTAATCA3310.19291517560526406No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTTGGTCAGGCTG2950.17193346466330184No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAAACTCTGTGA2850.16610521162386788No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAAGAAAATGTG2590.15095175372133957No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAATTAGATCCC2330.13579829581881125No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAAAAAGTCAGG2240.1305528680833207No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAATAACTAATG1990.11598223548473581No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAATTTTTTTTT1780.1037429041019245No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAACTCGTCATT1750.1019944281900943No Hit
TGACTATGGTTGCAAGAGACGAAGCTTCGTCTCAGTCTTAATCCAGTCTA1750.1019944281900943No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)