FastQCFastQC Report
Mon 27 Mar 2023
new_CS_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamenew_CS_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171578
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTCTCCTCATGGTCAGATGTCTAGAGGCCGCGTTGCTGGCGTTTTTC76134.4370490389210735No Hit
ATGTTCTCCTAGACTGAGACGAAGCTTCGTCTCTTGCAACCATAGTCA15240.8882257632097355No Hit
ATGTTCTCCTTAAGACTGAGACGAAGCTTCGTCTCTTGCAACCATAGTCA8720.5082236650386414No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8620.5023954119992073No Hit
ATGTTCTCCTCTTTGCATACTTCTGCCTGCTGGGGAGCCTGGGGACTTTC5000.29141265197169797No Hit
ATGTTCTCCTGACGAGCTGTACAAGTAAAGCGGCCGCGACTCTAGATCAT4910.28616722423620744No Hit
ATGTTCTCCTAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAA4380.25527748312720744No Hit
ATGTTCTCCTGTCAGATGTCTAGAGGCCGCGTTGCTGGCGTTTTTCCATA4260.2482835794798867No Hit
ATGTTCTCCTCTGGAGTTCTTCGCCCACCCTAGGGGGAGGCTAACTGAAA4070.23720989870496217No Hit
ATGTTCTCCTTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGA2650.15444870554499995No Hit
ATGTTCTCCTGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTA2240.1305528680833207No Hit
ATGTTCTCCTCTCGCCTTGAGCCTGGCGAACAGTTCGGCTGGCGCGAGCC2020.11773071139656599No Hit
ATGTTCTCCTTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTA1840.10723985592558487No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)