#1 #File: TAF4_chr3_only.vcf.gz #Description: TAF4_chr3_only.vcf.gz contains variant calls for TAF4.bam in 3 chromosome. #Command used: bcftools mpileup -a 'FORMAT/DP,FORMAT/SP,FORMAT/AD,FORMAT/ADF,FORMAT/ADR,INFO/AD,INFO/ADR,INFO/ADF' -r chr3 -f wcg38.fa TAF4.sorted.bam | bcftools call -cv -p 0.05 | bcftools filter -i 'QUAL>=20 && FORMAT/DP>=5' -Oz -o TAF4_chr3_only.vcf.gz #2 #File: TAF4_chr3_only.vcf.gz.tbi #Description: TAF4_chr3_only.vcf.gz.tbi is an index-file for TAF4_chr3_only.vcf.gz. #Command used: bcftools index -t TAF4_chr3_only.vcf.gz #3 #File: chip.vcf.gz #Description: chip.vcf.gz contains variant calls for all ChIP-seq data in 3 chromosome. #Command used: bcftools mpileup -a 'FORMAT/DP,FORMAT/SP,FORMAT/AD,FORMAT/ADF,FORMAT/ADR,INFO/AD,INFO/ADR,INFO/ADF' -r chr3 -f ../wcg38.fa input_iN_4-22_r1_L1_1.fq.gzinput_iN_4-22_r1_L1_2.fq.gz.bam iN_4-22_Cre2_r1_L1_1.fq.gziN_4-22_Cre2_r1_L1_2.fq.gz.bam iN_4-14_r1_L1_2.fq.gziN_4-14_r1_L1_1.fq.gz.bam iN1-25_r1_L1_2.fq.gziN1-25_r1_L1_1.fq.gz.bam iN_4-22_r2_L1_2.fq.gziN_4-22_r2_L1_1.fq.gz.bam input_iN_14-8_r1_L1_2.fq.gzinput_iN_14-8_r1_L1_1.fq.gz.bam input_iN_1-36_r2_L1_2.fq.gzinput_iN_1-36_r2_L1_1.fq.gz.bam input_iN_1-36_r1_L1_2.fq.gzinput_iN_1-36_r1_L1_1.fq.gz.bam delete/CB_wt_GGCTACCA_L1_1.fq.gzCB_wt_GGCTACCA_L1_2.fq.gz.bam input_iN_14-10_r1_L1_1.fq.gzinput_iN_14-10_r1_L1_2.fq.gz.bam input_iN_4-22_Cre2_r1_L1_1.fq.gzinput_iN_4-22_Cre2_r1_L1_2.fq.gz.bam CB_wt_TAGCTTCA_L1_2.fq.gzCB_wt_TAGCTTCA_L1_1.fq.gz.bam iN_1-36_r1_L1_2.fq.gziN_1-36_r1_L1_1.fq.gz.bam input_iN_14-10_r2_L1_2.fq.gzinput_iN_14-10_r2_L1_1.fq.gz.bam input_iN1-25_r1_L1_2.fq.gzinput_iN1-25_r1_L1_1.fq.gz.bam CB_wt_GGCTACCA_L1_1.fq.gzCB_wt_GGCTACCA_L1_2.fq.gz.bam iN_14-10_r1_L1_2.fq.gziN_14-10_r1_L1_1.fq.gz.bam iN_4-22_r1_L1_1.fq.gziN_4-22_r1_L1_2.fq.gz.bam input_iN_1-25_r2_L1_1.fq.gzinput_iN_1-25_r2_L1_2.fq.gz.bam iN_4-22_Cre2_r2_L1_2.fq.gziN_4-22_Cre2_r2_L1_1.fq.gz.bam iN_1-36_r2_L1_1.fq.gziN_1-36_r2_L1_2.fq.gz.bam | bcftools call -cv -p 0.05 | bcftools filter -i 'QUAL>=20 && FORMAT/DP>=5' -Oz -o chip.vcf.gz #4 #File: chip.vcf.gz.tbi #Description: chip.vcf.gz.tbi is an index-file for TAF4_chr3_only.vcf.gz. #Command used: bcftools index -t chip.vcf.gz #5 #File: chip4.vcf.gz #Description: chip4.vcf.gz contains variant calls for ChIP-seq data of son. #Command used: bcftools mpileup -a 'FORMAT/DP,FORMAT/SP,FORMAT/AD,FORMAT/ADF,FORMAT/ADR,INFO/AD,INFO/ADR,INFO/ADF' -r chr3 -f ../wcg38.fa input_iN_4-22_r1_L1_1.fq.gzinput_iN_4-22_r1_L1_2.fq.gz.bam iN_4-22_Cre2_r1_L1_1.fq.gziN_4-22_Cre2_r1_L1_2.fq.gz.bam iN_4-14_r1_L1_2.fq.gziN_4-14_r1_L1_1.fq.gz.bam iN_4-22_r2_L1_2.fq.gziN_4-22_r2_L1_1.fq.gz.bam input_iN_4-22_Cre2_r1_L1_1.fq.gzinput_iN_4-22_Cre2_r1_L1_2.fq.gz.bam iN_4-22_r1_L1_1.fq.gziN_4-22_r1_L1_2.fq.gz.bam iN_4-22_Cre2_r2_L1_2.fq.gziN_4-22_Cre2_r2_L1_1.fq.gz.bam | bcftools call -cv -p 0.05 | bcftools filter -i 'QUAL>=20 && FORMAT/DP>=5' -Oz -o chip4.vcf.gz #6 #File: chip4.vcf.gz.tbi #Description: chip4.vcf.gz.tbi is an index-file for chip4.vcf.gz. #Command used: bcftools index -t chip4.vcf.gz #7 #File: chip14.vcf.gz #Description: chip14.vcf.gz contains variant calls for ChIP-seq data of father. #Command used: bcftools mpileup -a 'FORMAT/DP,FORMAT/SP,FORMAT/AD,FORMAT/ADF,FORMAT/ADR,INFO/AD,INFO/ADR,INFO/ADF' -r chr3 -f ../wcg38.fa input_iN_14-8_r1_L1_2.fq.gzinput_iN_14-8_r1_L1_1.fq.gz.bam input_iN_14-10_r1_L1_1.fq.gzinput_iN_14-10_r1_L1_2.fq.gz.bam input_iN_14-10_r2_L1_2.fq.gzinput_iN_14-10_r2_L1_1.fq.gz.bam iN_14-10_r1_L1_2.fq.gziN_14-10_r1_L1_1.fq.gz.bam | bcftools call -cv -p 0.05 | bcftools filter -i 'QUAL>=20 && FORMAT/DP>=5' -Oz -o chip14.vcf.gz #8 #File: chip14.vcf.gz.tbi #Description: chip14.vcf.gz.tbi is an index-file for chip14.vcf.gz. #Command used: bcftools index -t chip14.vcf.gz #9 #File: chip4_positive.vcf #Description: chip4_positive.vcf contains variant calls of the patient that definitely came from the father. (based on chip4.vcf) #Command used: #10 #File: chip4_positive_extended.vcf #Description: chip4_positive.vcf contains variant calls of the patient that definitely or probably came from the father (situation of 0/1 & 0/1 included). (based on chip4.vcf) #Command used: #11 #File: TAF4_positive.vcf #Description: chip4_positive.vcf contains variant calls of the patient that definitely came from the father. (based on TAF4_chr3_only.vcf) #Command used: #12 #File: TAF4_positive_extended.vcf #Description: chip4_positive.vcf contains variant calls of the patient that definitely or probably came from the father (situation of 0/1 & 0/1 included). (based on TAF4_chr3_only.vcf) #Command used: