Sample information : Larus_michahellis
- Estimated number of cells
- The total number of barcodes identified as cells.
- Median UMI counts per cell
- Among barcodes identified as cells, the median number of UMI per cell.
- Median genes per cell
- The median number of genes per cell.
- Mean reads per cell
- The mean number of reads per cell.
465
Estimated number of cell
11,468
Median UMI counts per cell
3,175
Median genes per cell
27,776
Mean reads per cell
Beads to cells
- (left) Beads to cells plot
- In library, different mRNAs from the same cell will carry the same cell barcode and random unique molecular identifiers (UMIs). However, some mRNA from dead cells mixed in reflection system was inevitability. This graph shows the distribution of UMI counts in each barcode. Barcodes can be determined to be cell-associated based on their UMI counts or by their expression profiles. Therefore, the graph contains both cell-associated (Colored Regions) and background-associated barcodes (Gray Regions).
- (right) Histogram
- Histograms of number of beads per droplet.
Summary
- Estimated number of cells
- The total number of barcodes identified as cells.
- Mean reads per cell
- The mean number of reads detected per cell.
- Mean UMI counts per cell
- The average number of UMI counts per cell. This item refers the average abundance of expression in each cell.
- Median UMI counts per cell
- The median number of UMI counts per cell.
- Total genes detected
- The total number of genes detected.
- Mean genes per cell
- The mean number of genes detected per cell. This item refers the activity of gene expression in each cell.
- Median genes per cell
- The median number of genes detected per cell.
- Sequencing saturation
- The fraction of UMI originating from an already-observed UMI.
- Fraction reads in cells
- The fraction of uniquely-mapped-to-transcriptome reads with cell-associated barcode.
- Plot
- The distribution of nFeature and nCount in violin.
Sample name
Larus_michahellis
Species
Larus_michahellis
Estimated number of cells
465
Mean reads per cell
27,776
Mean UMI count per cell
13,076
Median UMI counts per cell
11,468
Total genes detected
14,356
Mean genes per cell
3,205
Median genes per cell
3,175
Fraction reads in cells
99.17%
Sequencing saturation
40.43%
Sequencing
- Number of reads
- Number of raw off-machine reads obtained by this sequencing program.
- Reads pass QC
- Number of reads after quality control (QC) that can be used for downstream analysis. QC includes filtering low quality reads and invalid barcodes.
- Reads with exactly matched barcodes
- Number of reads with barcodes that match the barcode whitelist before correction.
- Reads with failed barcodes
- Number of reads with invalid barcodes that fail to match the barcode whitelist.
- Reads filtered on low quality
- Number of reads in low quality. The QC will filter the average base quality < Q20 or 2 bases < Q10 in the first 15 bases.
- Q30 bases in cell barcode
- Fraction of cell barcode bases with Q-score ≥ 30.
- Q30 bases in UMI
- Fraction of UMI bases with Q-score ≥ 30.
- Q30 bases in reads
- Fraction of RNA read bases with Q-score ≥ 30.
mRNA
Number of reads
21,604,344
Reads pass QC
95.41%
Reads with exactly matched barcodes
100.0%
Reads with failed barcodes
0.0%
Reads filtered on low quality
0.0%
Q30 bases in cell barcode
88.41%
Q30 bases in UMI
88.36%
Q30 bases in reads
93.10%
Droplet index
Number of reads
218,724,924
Reads pass QC
96.18%
Reads with exactly matched barcodes
90.21%
Reads with failed barcodes
3.26%
Reads filtered on low quality
0.56%
Q30 bases in cell barcode
95.89%
Q30 bases in reads
96.75%
Mapping & Annotation
- Reads pass QC
- Number of reads after quality control (QC) that can be used for downstream analysis. QC includes filtering low quality reads and invalid barcodes.
- Reads mapped to genome
- Fraction of reads that mapped to the genome.
- Plus strand
- The number of reads in plus strand.
- Minus strand
- The number of reads in minus strand.
- Mitochondria ratio
- The fraction of reads mapped to mitochondria.
- Mapping quality corrected reads
- The number of reads that align to a single exonic locus but also align to 1 or more non-exonic loci.
- Reads mapped to exonic regionse
- Fraction of reads that mapped to an exonic region of genome.
- Reads mapped to intronic regions
- Fraction of reads that mapped to an intronic region of genome.
- Reads mapped antisense to gene
- Fraction of reads mapped to transcriptome, but on the opposite strand of their annotated gene. This part of reads will be filtered out cause them are opposite to theoretical direction.
- Reads mapped to intergenic regions
- Fraction of reads that mapped to intergenic regions of genome.
Reads pass QC
20,612,408
Reads mapped to genome
63.38%
Plus strand
51.01%
Minus strand
48.99%
Mitochondria ratio
0.00%
Mapping quality corrected reads
0.03%
Reads mapped to exonic regions
82.1%
Reads mapped to intronic regions
8.9%
Reads mapped antisense to gene
11.3%
Reads mapped to intergenic regions
9.0%
Include introns
True
Cluster
- Left
- The figure is automated clustering each cell-barcode by UMAP algorithm. The cells clustered into the same group have similar expression profiles. Each dot represents a cell, and is colored according to different cluster.
- Right
- This plot shows that the total UMI counts for each cell-barcode. Two-dimensional horizontal and vertical coordinates of each dot are obtained using the uniform manifold approximation and projection (UMAP) algorithm. Each dot represents a cell and is colored according to UMI counts. Cells with greater UMI counts likely have higher RNA content than cells with fewer ones.
Marker
- Table
- The table shows the top 30 differentially expressed genes for each cluster. Here a differential expression test was performed between each cluster and the rest of the sample for each gene. The p-value is a measure of the statistical significance of the expression difference, the smaller the P value, the closer to the theoretical value. Avg_log2FC is log fold-chage of the average expression between the markers for every cluster compared to all remaining cells.
| gene | cluster | p_val_adj | p_val | avg_log2FC | pct.1 | pct.2 |
|---|---|---|---|---|---|---|
| PCDH10 | 0 | 0.0007019052198164049 | 9.757519884838084e-06 | 4.675 | 0.287 | 0.017 |
| CPM | 0 | 0.02798486318685712 | 0.0009110729159774386 | 4.326 | 0.225 | 0.024 |
| LOC141749978 | 0 | 0.06158052950123095 | 0.002539100078137199 | 3.913 | 0.209 | 0.028 |
| DKK2 | 0 | 0.012347053501051102 | 0.00033513852136101454 | 3.784 | 0.24 | 0.021 |
| ALDH1A3 | 0 | 2.8103114808250697e-16 | 2.236974831509249e-19 | 3.59 | 0.651 | 0.149 |
| TWIST2 | 0 | 9.385142622915354e-15 | 8.964555557986489e-18 | 3.017 | 0.651 | 0.153 |
| WNT9A | 0 | 1.1386991243159476e-21 | 2.719173265102159e-25 | 2.999 | 0.783 | 0.219 |
| CRABP1 | 0 | 6.521651035852086e-10 | 1.5573472186226425e-12 | 2.902 | 0.55 | 0.135 |
| LOC141736464 | 0 | 0.015008660841616934 | 0.0004193297743697798 | 2.792 | 0.256 | 0.038 |
| CEMIP | 0 | 6.494861239127048e-10 | 1.4992515795167109e-12 | 2.762 | 0.566 | 0.149 |
| LOC141743671 | 0 | 5.487134413847031e-06 | 3.756217142329417e-08 | 2.678 | 0.426 | 0.094 |
| PDGFD | 0 | 3.866287490587089e-20 | 1.2310077180887014e-23 | 2.584 | 0.829 | 0.295 |
| NTN1 | 0 | 0.004896450333223661 | 0.000103284194722938 | 2.579 | 0.302 | 0.069 |
| LOC141744712 | 0 | 6.506625256523624e-17 | 3.741025705017425e-20 | 2.541 | 0.76 | 0.267 |
| MXRA5 | 0 | 1.6168652156789591e-18 | 6.970195117215336e-22 | 2.472 | 0.868 | 0.41 |
| IRX5 | 0 | 0.002060993209409354 | 3.5763473664828405e-05 | 2.455 | 0.326 | 0.076 |
| PTX3 | 0 | 1.0940933377868201e-23 | 1.7417708155485476e-27 | 2.42 | 0.969 | 0.604 |
| F2R | 0 | 0.019390138908656752 | 0.0005880476736606083 | 2.305 | 0.287 | 0.083 |
| LIMD2 | 0 | 0.0032406942268788292 | 6.319908346615563e-05 | 2.295 | 0.318 | 0.073 |
| CTBP2 | 0 | 6.202026416107595e-12 | 9.873479926940373e-15 | 2.266 | 0.667 | 0.264 |
| PRDM1 | 0 | 6.524859465889276e-11 | 1.3503649296594857e-13 | 2.242 | 0.62 | 0.177 |
| WNT2B | 0 | 0.005874215994615861 | 0.0001323253304526219 | 2.185 | 0.31 | 0.076 |
| C3H1orf198 | 0 | 3.9209588721041184e-25 | 3.1210370708462297e-29 | 2.155 | 0.969 | 0.604 |
| CACNB2 | 0 | 0.05673341360869678 | 0.002289567834084953 | 2.136 | 0.233 | 0.045 |
| HAND2 | 0 | 4.2671833627989e-10 | 9.510557522754852e-13 | 2.114 | 0.667 | 0.302 |
| AKAP12 | 0 | 1.9331830506109054e-12 | 2.7698236815248183e-15 | 2.106 | 0.713 | 0.292 |
| LOC141743672 | 0 | 0.00029278481814206184 | 3.7521575834491725e-06 | 2.101 | 0.388 | 0.104 |
| DIO2 | 0 | 1.6168652156789591e-18 | 7.722033983979746e-22 | 2.076 | 0.907 | 0.458 |
| LOC141739670 | 0 | 0.03306925800279881 | 0.0011160841672519855 | 2.013 | 0.271 | 0.069 |
| ADGRA2 | 0 | 0.002657118749475424 | 4.993816291836635e-05 | 2.01 | 0.349 | 0.104 |
| NRP1 | 0 | 5.543105838277671e-11 | 1.1030617365035564e-13 | 1.918 | 0.705 | 0.323 |
| ADAMTS2 | 0 | 6.539072569412943e-08 | 2.602512365443343e-10 | 1.913 | 0.605 | 0.288 |
| ROR2 | 0 | 0.0003812423842817043 | 4.976816924476599e-06 | 1.909 | 0.411 | 0.139 |
| THBS1 | 0 | 1.5765594107709409e-16 | 1.1294304463056967e-19 | 1.858 | 0.969 | 0.785 |
| FBLN5 | 0 | 2.589979147841606e-06 | 1.624501106273879e-08 | 1.812 | 0.519 | 0.174 |
| NR2F2 | 0 | 3.9169061744640104e-16 | 3.429592288394819e-19 | 1.805 | 0.891 | 0.559 |
| FBLN1 | 0 | 7.25320274506498e-12 | 1.2701620663171977e-14 | 1.771 | 0.822 | 0.42 |
| PDE1C | 0 | 0.009795345321963285 | 0.0002471642495472706 | 1.755 | 0.326 | 0.101 |
| COL3A1 | 0 | 1.0998493336723952e-05 | 8.404484281823604e-08 | 1.724 | 0.558 | 0.267 |
| ALAS1 | 0 | 1.587413961508207e-05 | 1.288834068883524e-07 | 1.723 | 0.535 | 0.25 |
| GAS6 | 0 | 0.0003902058445670386 | 5.124887714205315e-06 | 1.699 | 0.442 | 0.17 |
| LOC141742208 | 0 | 1.3418718838191123e-13 | 1.4953598960015578e-16 | 1.682 | 0.829 | 0.507 |
| HLF | 0 | 6.373350439491174e-06 | 4.5150695623236053e-08 | 1.649 | 0.574 | 0.285 |
| CDCP1 | 0 | 0.015932541363754552 | 0.00045401972524270715 | 1.644 | 0.333 | 0.122 |
| CAV2 | 0 | 0.04912077971773901 | 0.0019013212648809664 | 1.621 | 0.295 | 0.108 |
| CSRP2 | 0 | 2.0953721573132239e-13 | 2.6686264838821604e-16 | 1.619 | 0.953 | 0.753 |
| KIAA0513 | 0 | 0.01798150028053744 | 0.0005324459209074208 | 1.612 | 0.341 | 0.135 |
| PRICKLE1 | 0 | 6.205747114364952e-05 | 5.680656834768523e-07 | 1.551 | 0.535 | 0.253 |
| TBX2 | 0 | 2.6907741412248457e-11 | 4.926196390047875e-14 | 1.535 | 0.806 | 0.545 |
| HIC1 | 0 | 0.0027655523290187763 | 5.283232977509403e-05 | 1.521 | 0.419 | 0.181 |
| C1QTNF3 | 1 | 0.0007961826704564193 | 1.1027285255067815e-05 | 1.883 | 0.404 | 0.122 |
| CAMKV | 1 | 1.5053863495993894e-07 | 5.512041079485148e-10 | 1.673 | 0.702 | 0.36 |
| FHL1 | 1 | 3.18538979058514e-09 | 5.32461876958433e-12 | 1.521 | 0.789 | 0.475 |
| LIPC | 1 | 0.050399499466869 | 0.0017772011672230333 | 1.49 | 0.342 | 0.149 |
| ITGA3 | 1 | 9.497040989836068e-09 | 1.814287859237966e-11 | 1.485 | 0.781 | 0.488 |
| RSPO3 | 1 | 8.797915384770893e-09 | 1.6106985103059028e-11 | 1.338 | 0.921 | 0.693 |
| LOC141737113 | 1 | 0.00020564192550738265 | 2.368172333702488e-06 | 1.277 | 0.588 | 0.323 |
| UCHL1 | 1 | 5.035086633434925e-06 | 3.37152334644388e-08 | 1.197 | 0.781 | 0.554 |
| CDK2AP1 | 1 | 0.04386826179979739 | 0.0014735657469962984 | 1.197 | 0.43 | 0.267 |
| ACTG2 | 1 | 0.0042738185472655545 | 8.096543932573446e-05 | 1.119 | 0.64 | 0.469 |
| LOC141750138 | 1 | 7.23997561030943e-05 | 7.376557176785855e-07 | 1.087 | 0.702 | 0.488 |
| IL34 | 1 | 0.030842022015469317 | 0.0009157107547377262 | 1.08 | 0.482 | 0.31 |
| PDLIM1 | 1 | 2.4668994395951745e-05 | 2.0618040369139005e-07 | 1.039 | 0.754 | 0.564 |
| NEFL | 1 | 0.02048691160499242 | 0.0005593417719105628 | 1.025 | 0.5 | 0.314 |
| CRIP1 | 1 | 4.514091979481771e-05 | 4.311796844207693e-07 | 0.989 | 0.991 | 0.914 |
| AHNAK2 | 1 | 0.0012397005822099762 | 1.874895412082269e-05 | 0.989 | 0.798 | 0.564 |
| TGFB2 | 1 | 8.270649843116365e-07 | 4.2133374986822205e-09 | 0.972 | 0.947 | 0.828 |
| PDRG1 | 1 | 0.005514213166031534 | 0.00010885336823814538 | 0.914 | 0.614 | 0.403 |
| PRLH | 1 | 0.07273940914753516 | 0.002941305408497004 | 0.914 | 0.561 | 0.406 |
| LOC141739185 | 1 | 0.08497500963146781 | 0.0036728034888073723 | 0.9 | 0.5 | 0.356 |
| C1QTNF12 | 1 | 1.8450400770796735e-05 | 1.4245712606600996e-07 | 0.88 | 0.904 | 0.769 |
| LOC141732862 | 1 | 0.07109799429817633 | 0.002863614193654161 | 0.877 | 0.518 | 0.356 |
| NICN1 | 1 | 0.001343436599938021 | 2.0638642345621116e-05 | 0.874 | 0.693 | 0.531 |
| LOC141735490 | 1 | 2.633987029372662e-10 | 1.8869603808289387e-13 | 0.812 | 1.0 | 0.957 |
| RPL10A | 1 | 3.5767520887697473e-13 | 1.423526263141665e-16 | 0.784 | 1.0 | 0.977 |
| ANXA2 | 1 | 1.120120330007657e-17 | 8.91602586967808e-22 | 0.775 | 1.0 | 0.993 |
| LOC141735480 | 1 | 3.510638778251439e-07 | 1.5928234526811434e-09 | 0.767 | 0.991 | 0.964 |
| LGALS3 | 1 | 1.8766919710170428e-05 | 1.4639482063175213e-07 | 0.754 | 0.956 | 0.884 |
| DIO3 | 1 | 0.03101131022638464 | 0.0009232054465229528 | 0.748 | 0.737 | 0.653 |
| VPS33B | 1 | 0.036980755087748396 | 0.0011539008194219034 | 0.747 | 0.632 | 0.475 |
| COX7A2 | 1 | 1.7169623327259079e-06 | 1.0250113424694985e-08 | 0.739 | 0.982 | 0.888 |
| COTL1 | 1 | 3.2821830068770205e-06 | 2.0900632058438403e-08 | 0.729 | 0.974 | 0.931 |
| RPL23A | 1 | 3.00835036956115e-17 | 4.7892229078423156e-21 | 0.715 | 1.0 | 1.0 |
| TAGLN | 1 | 0.0034138217944813475 | 5.923849010562237e-05 | 0.704 | 0.991 | 0.894 |
| GCSH | 1 | 0.06660680628262265 | 0.0026456098332427527 | 0.7 | 0.675 | 0.531 |
| RPL22L1 | 1 | 0.0002069964929067071 | 2.405594839160968e-06 | 0.692 | 0.939 | 0.868 |
| RPL36 | 1 | 4.833760097401898e-13 | 2.308569655688242e-16 | 0.687 | 1.0 | 1.0 |
| BOLA3 | 1 | 5.035086633434925e-06 | 3.3540727165213404e-08 | 0.687 | 0.939 | 0.875 |
| LOC141734506 | 1 | 3.407094365378475e-13 | 1.0848027908552017e-16 | 0.686 | 1.0 | 0.99 |
| RPS21 | 1 | 2.4235830591442366e-09 | 3.665372771132731e-12 | 0.68 | 1.0 | 0.99 |
| SHISA2 | 1 | 0.08926661060880645 | 0.003943561958758861 | 0.675 | 0.605 | 0.472 |
| LOC141735830 | 1 | 1.7961733823444103e-07 | 7.148664261499683e-10 | 0.657 | 0.982 | 0.977 |
| CSRP1 | 1 | 2.4879020974192215e-07 | 1.0495806030662959e-09 | 0.656 | 0.991 | 0.97 |
| ESD | 1 | 0.0056150655156521284 | 0.0001113216681812701 | 0.645 | 0.763 | 0.67 |
| GM2A | 1 | 0.09087058061339946 | 0.004065053435065709 | 0.63 | 0.675 | 0.561 |
| ELOF1 | 1 | 0.0007467295398928029 | 1.0223472169192239e-05 | 0.624 | 0.912 | 0.835 |
| MICOS13 | 1 | 0.00020564192550738265 | 2.3628721044378333e-06 | 0.621 | 0.965 | 0.865 |
| RAB11B | 1 | 0.08131679098487624 | 0.0034240692852821404 | 0.621 | 0.684 | 0.548 |
| ACTG1 | 1 | 1.4024120796304532e-14 | 3.3489104822823846e-18 | 0.62 | 1.0 | 0.997 |
| RPL7 | 1 | 4.3828644813776937e-10 | 4.8841918920073e-13 | 0.617 | 1.0 | 0.98 |
| HMMR | 2 | 0.09891025450100184 | 0.0077314232205670476 | 27.404 | 0.203 | 0.0 |
| CCNA2 | 2 | 0.004745306395340141 | 0.00015788729389892375 | 8.372 | 0.29 | 0.003 |
| PLK1 | 2 | 3.0128374394751557e-05 | 3.2855108589357347e-07 | 8.047 | 0.391 | 0.003 |
| AURKA | 2 | 0.026814643755696303 | 0.0013617561662130258 | 7.09 | 0.246 | 0.003 |
| NUSAP1 | 2 | 0.0008377766274636859 | 1.862627298837935e-05 | 6.111 | 0.333 | 0.009 |
| SPAG5 | 2 | 0.005443444185506719 | 0.00018761532534620787 | 6.071 | 0.29 | 0.006 |
| DLGAP5 | 2 | 1.0782167808012381e-06 | 7.724230699045724e-09 | 6.06 | 0.449 | 0.011 |
| UBE2C | 2 | 6.99183396596202e-09 | 2.560092035614526e-11 | 6.032 | 0.522 | 0.017 |
| KIF2C | 2 | 0.048891839852324044 | 0.0029071244423852635 | 5.974 | 0.232 | 0.006 |
| CDK1 | 2 | 8.15265955972864e-06 | 7.787305159336438e-08 | 5.949 | 0.42 | 0.014 |
| KPNA2 | 2 | 2.660330423152365e-05 | 2.850453857695766e-07 | 5.845 | 0.406 | 0.02 |
| CDCA8 | 2 | 0.051935582617131125 | 0.0031940706924656302 | 5.771 | 0.232 | 0.009 |
| BUB1B | 2 | 0.006984341248672523 | 0.00025741534662922916 | 5.553 | 0.29 | 0.014 |
| TTK | 2 | 0.006174505035523067 | 0.0002196930471128561 | 5.359 | 0.29 | 0.009 |
| CDCA3 | 2 | 5.496027690450049e-05 | 7.218375936673232e-07 | 5.309 | 0.391 | 0.017 |
| ANLN | 2 | 2.181493442872506e-06 | 1.7017142195455352e-08 | 5.179 | 0.449 | 0.029 |
| KIF20B | 2 | 3.361833048664718e-07 | 2.087264011747576e-09 | 5.05 | 0.478 | 0.029 |
| KIF4A | 2 | 0.002364799578713212 | 6.399998859846311e-05 | 5.031 | 0.319 | 0.017 |
| SKA3 | 2 | 0.05649096116348146 | 0.003606284394898681 | 4.97 | 0.232 | 0.011 |
| CENPF | 2 | 7.873909277417909e-06 | 7.395696049791558e-08 | 4.88 | 0.435 | 0.032 |
| MKI67 | 2 | 2.3247102640858143e-05 | 2.442583418445654e-07 | 4.858 | 0.42 | 0.04 |
| SHCBP1 | 2 | 0.07756926663836627 | 0.005649596352312755 | 4.84 | 0.217 | 0.006 |
| CDC20 | 2 | 0.0026042717235785252 | 7.296853034304233e-05 | 4.833 | 0.319 | 0.02 |
| KIF15 | 2 | 0.08814014888088958 | 0.00667207526750983 | 4.678 | 0.217 | 0.011 |
| FAM72A | 2 | 0.05810030151901182 | 0.00377376789298047 | 4.669 | 0.232 | 0.011 |
| CKAP2 | 2 | 1.5554978495294478e-06 | 1.163868485678326e-08 | 4.607 | 0.464 | 0.037 |
| INCENP | 2 | 0.0027903291562232694 | 7.995848891509806e-05 | 4.535 | 0.319 | 0.02 |
| SPC25 | 2 | 0.09347298268901294 | 0.007172487090042862 | 4.511 | 0.217 | 0.014 |
| BUB1 | 2 | 0.03800377031936654 | 0.0020993884105420984 | 4.403 | 0.246 | 0.011 |
| CCNB1 | 2 | 3.2379044822952555e-07 | 1.9845470439921566e-09 | 4.372 | 0.493 | 0.043 |
| ERCC6L | 2 | 0.05868915559317609 | 0.0038400450447815602 | 4.284 | 0.232 | 0.011 |
| DEPDC1 | 2 | 0.028530896454687323 | 0.0014716246838046155 | 4.217 | 0.261 | 0.02 |
| CKAP2L | 2 | 0.0062941291578357626 | 0.00022495136447251907 | 4.141 | 0.304 | 0.026 |
| PIMREG | 2 | 4.883670593310113e-06 | 4.3538255707293847e-08 | 4.111 | 0.449 | 0.037 |
| KNL1 | 2 | 0.04845828716391545 | 0.0028659163705157353 | 3.952 | 0.246 | 0.02 |
| TOP2A | 2 | 1.5452605674348322e-05 | 1.599011969804411e-07 | 3.91 | 0.464 | 0.08 |
| NUF2 | 2 | 0.03063577790793826 | 0.0016045802645405854 | 3.906 | 0.261 | 0.02 |
| CENPE | 2 | 0.007998809398950263 | 0.0003043405948179754 | 3.894 | 0.304 | 0.034 |
| PRC1 | 2 | 0.00011607045561677329 | 1.7923798765942862e-06 | 3.855 | 0.406 | 0.049 |
| PBK | 2 | 9.899831256905032e-08 | 5.043295394745857e-10 | 3.839 | 0.522 | 0.055 |
| CENPU | 2 | 0.0023947775683826646 | 6.519254384994598e-05 | 3.828 | 0.333 | 0.032 |
| CENPW | 2 | 0.03541663170297109 | 0.001925460435654641 | 3.803 | 0.261 | 0.026 |
| CKS2 | 2 | 0.025518893883624338 | 0.0012715774553171085 | 3.727 | 0.275 | 0.034 |
| TK1 | 2 | 0.03964278160292977 | 0.0022057075810274543 | 3.637 | 0.261 | 0.032 |
| KIF11 | 2 | 0.015079136927418073 | 0.000656553999784899 | 3.63 | 0.29 | 0.034 |
| PTTG1 | 2 | 0.0005011898468301347 | 1.0013424465045276e-05 | 3.615 | 0.377 | 0.049 |
| SMC2 | 2 | 2.4836402069000723e-12 | 3.756201857128184e-15 | 3.56 | 0.681 | 0.112 |
| ZWILCH | 2 | 0.06276387204329932 | 0.0042165651519975025 | 3.497 | 0.246 | 0.029 |
| CENPH | 2 | 4.879073618692099e-07 | 3.223458651209458e-09 | 3.448 | 0.507 | 0.06 |
| RRM2 | 2 | 0.0011754362483582359 | 2.7628074186929725e-05 | 3.297 | 0.362 | 0.043 |
| COL11A2 | 3 | 1.3450130517283103e-06 | 2.788226259622702e-09 | 5.875 | 0.509 | 0.031 |
| COL9A1 | 3 | 0.09418517821425926 | 0.0017853619479385606 | 5.824 | 0.263 | 0.006 |
| SPP1 | 3 | 0.004624466415156122 | 3.7546412031037526e-05 | 4.727 | 0.368 | 0.033 |
| AKR1D1 | 3 | 4.93858497044361e-08 | 7.075899822334233e-11 | 4.286 | 0.596 | 0.103 |
| BMP7 | 3 | 2.065583212092415e-11 | 1.150925932074099e-14 | 4.282 | 0.702 | 0.117 |
| FABP7 | 3 | 0.04098938960167262 | 0.0005774991609883033 | 3.871 | 0.316 | 0.039 |
| MEF2C | 3 | 0.007483650132256023 | 6.552587077514626e-05 | 3.83 | 0.368 | 0.056 |
| SOX8 | 3 | 6.427638023208192e-13 | 2.0465296579505508e-16 | 3.789 | 0.754 | 0.117 |
| COL2A1 | 3 | 0.00033534609958668323 | 1.6549755770416588e-06 | 3.74 | 0.474 | 0.117 |
| LOC141751688 | 3 | 2.2163689364004206e-16 | 5.292610689486e-20 | 3.583 | 0.982 | 0.725 |
| KCNMB1 | 3 | 0.00921510025588102 | 8.948835717722552e-05 | 3.439 | 0.368 | 0.053 |
| FGFR3 | 3 | 0.0759818810186146 | 0.0013431866900172813 | 3.395 | 0.298 | 0.036 |
| TIMP4 | 3 | 0.018059812736693093 | 0.00021275589310121607 | 3.315 | 0.351 | 0.05 |
| LOXL3 | 3 | 0.003640345673417382 | 2.83971882508082e-05 | 3.17 | 0.404 | 0.069 |
| FNDC1 | 3 | 2.1542860215157984e-08 | 2.4007008120051877e-11 | 3.112 | 0.719 | 0.236 |
| FAT3 | 3 | 0.00245683499541421 | 1.6622699563019012e-05 | 3.073 | 0.421 | 0.075 |
| LOC141742143 | 3 | 1.6729436892295973e-05 | 4.927080832722686e-08 | 2.934 | 0.544 | 0.128 |
| GBGT1 | 3 | 0.002018859154120305 | 1.3016603636372262e-05 | 2.813 | 0.439 | 0.094 |
| LRRC17 | 3 | 0.02917669309851208 | 0.00038929344660965467 | 2.677 | 0.368 | 0.092 |
| SPON1 | 3 | 0.0001990989256310085 | 9.033382759665275e-07 | 2.56 | 0.561 | 0.208 |
| MAFB | 3 | 1.2537161158993694e-16 | 1.9958865173913387e-20 | 2.458 | 0.982 | 0.75 |
| BAMBI | 3 | 0.0004427845450678272 | 2.2909333303676482e-06 | 2.399 | 0.526 | 0.169 |
| PTPRF | 3 | 0.07545025573216729 | 0.0013212653236549237 | 2.366 | 0.333 | 0.069 |
| SERPINF1 | 3 | 0.002272556058078097 | 1.5014101155813263e-05 | 2.363 | 0.491 | 0.158 |
| COL11A1 | 3 | 1.5866918235771821e-12 | 7.57792799607028e-16 | 2.345 | 0.93 | 0.578 |
| ADAMTS10 | 3 | 0.001125166073952986 | 6.627580153826393e-06 | 2.229 | 0.509 | 0.153 |
| ASS1 | 3 | 0.006697487065827142 | 5.8109216761534546e-05 | 2.16 | 0.456 | 0.133 |
| ASPN | 3 | 0.0575588520077208 | 0.0009346498296246949 | 2.155 | 0.421 | 0.178 |
| DACT1 | 3 | 0.004134934320731609 | 3.258445417117163e-05 | 2.143 | 0.491 | 0.186 |
| QPCT | 3 | 0.009412952160803682 | 9.215896806326935e-05 | 2.131 | 0.456 | 0.161 |
| FAM20A | 3 | 0.028872330730866237 | 0.00038150178311898396 | 2.114 | 0.421 | 0.153 |
| NID1 | 3 | 0.04717780257983795 | 0.0007097512288139277 | 2.031 | 0.386 | 0.114 |
| THBS2 | 3 | 0.00020471600050689192 | 9.451188433813365e-07 | 2.0 | 0.596 | 0.217 |
| MDK | 3 | 2.8571541535919145e-07 | 4.775948199110897e-10 | 1.994 | 0.93 | 0.6 |
| LOC141741928 | 3 | 0.046412192425961424 | 0.0006871501863590563 | 1.964 | 0.404 | 0.139 |
| LOC141740987 | 3 | 0.08936809858469558 | 0.001650354124942241 | 1.958 | 0.386 | 0.147 |
| ACTG2 | 3 | 7.940575808473024e-06 | 2.085799583535858e-08 | 1.956 | 0.807 | 0.469 |
| COL9A2 | 3 | 0.001125166073952986 | 6.627580153826393e-06 | 1.951 | 0.614 | 0.317 |
| LOC141735173 | 3 | 0.0604271999657555 | 0.0009956563235621577 | 1.916 | 0.404 | 0.15 |
| FKBP1B | 3 | 0.04985457754274095 | 0.0007619262029934142 | 1.826 | 0.439 | 0.217 |
| CDH2 | 3 | 1.7297124131726172e-07 | 2.7536614075819744e-10 | 1.818 | 0.842 | 0.492 |
| GNG10 | 3 | 1.1715031089809018e-05 | 3.263759357982294e-08 | 1.767 | 0.702 | 0.328 |
| PRLH | 3 | 0.0017242970845539104 | 1.0705657294850355e-05 | 1.758 | 0.684 | 0.411 |
| DDIT4 | 3 | 4.025127455328887e-06 | 9.932257511358393e-09 | 1.734 | 0.86 | 0.703 |
| ST3GAL1 | 3 | 1.856975636168944e-06 | 4.1387660441558885e-09 | 1.662 | 0.807 | 0.483 |
| RSPRY1 | 3 | 0.005214614536928963 | 4.366317533664783e-05 | 1.633 | 0.561 | 0.286 |
| LOC141742880 | 3 | 0.028337299627287014 | 0.0003721765850913283 | 1.632 | 0.509 | 0.269 |
| CAPG | 3 | 0.005608352793294203 | 4.821317373842028e-05 | 1.606 | 0.544 | 0.25 |
| PHETA2 | 3 | 0.002844196236611494 | 2.1168979648318448e-05 | 1.585 | 0.614 | 0.342 |
| COL8A1 | 3 | 0.014673187417144382 | 0.00016234761211359302 | 1.531 | 0.632 | 0.461 |
| PHF21B | 4 | 0.003620652702825118 | 9.914069328757785e-05 | 6.691 | 0.433 | 0.01 |
| LOC141743810 | 4 | 0.0005757837399136752 | 1.1274600017413365e-05 | 5.182 | 0.5 | 0.021 |
| PRDM16 | 4 | 5.016276518270136e-06 | 4.791476416400671e-08 | 5.12 | 0.633 | 0.062 |
| LOC141732160 | 4 | 0.0007084764851211753 | 1.40420795029191e-05 | 4.934 | 0.5 | 0.034 |
| SLCO3A1 | 4 | 0.0012101674518532972 | 2.6310848664694662e-05 | 4.565 | 0.5 | 0.054 |
| RBFOX1 | 4 | 0.00014020817298017886 | 2.1427978358826985e-06 | 4.474 | 0.567 | 0.07 |
| LOC141746179 | 4 | 0.0707857006292574 | 0.004107520159096446 | 4.449 | 0.333 | 0.021 |
| LOC141733732 | 4 | 0.008882406066785207 | 0.00029836626284990503 | 4.38 | 0.433 | 0.059 |
| ESRRG | 4 | 0.05035175004111791 | 0.0025811133508302103 | 4.308 | 0.367 | 0.052 |
| DOCK5 | 4 | 0.0529163958391852 | 0.002746277966023143 | 4.292 | 0.367 | 0.052 |
| PTPRK | 4 | 0.0003987810997456094 | 7.300776322653042e-06 | 4.282 | 0.533 | 0.067 |
| JARID2 | 4 | 5.5894760799982384e-05 | 7.474583948417607e-07 | 4.202 | 0.6 | 0.101 |
| MEF2D | 4 | 3.7559790187284764e-09 | 7.773259132925288e-12 | 4.182 | 0.833 | 0.238 |
| P3H2 | 4 | 0.053719745089395726 | 0.002817902731346954 | 4.112 | 0.367 | 0.052 |
| ZNF407 | 4 | 0.06856682311459032 | 0.003907812254242352 | 3.878 | 0.367 | 0.075 |
| PEBP4 | 4 | 0.002256834607295983 | 5.497026107080879e-05 | 3.873 | 0.533 | 0.155 |
| EBF1 | 4 | 2.6621570452076633e-06 | 2.309759754259614e-08 | 3.851 | 0.733 | 0.261 |
| HDAC9 | 4 | 1.3702820035803695e-06 | 1.0907283320706595e-08 | 3.833 | 0.7 | 0.121 |
| SARDH | 4 | 0.06862261025711709 | 0.003917584309628031 | 3.812 | 0.367 | 0.072 |
| LOC141734508 | 4 | 0.009994300775349074 | 0.00034287539872446477 | 3.81 | 0.467 | 0.121 |
| ABTB2 | 4 | 0.06672575817683772 | 0.003792262305043551 | 3.794 | 0.367 | 0.059 |
| LOC141749555 | 4 | 0.0017755530649892293 | 4.141025615233974e-05 | 3.788 | 0.533 | 0.134 |
| OSBP2 | 4 | 0.0037693031998530125 | 0.00010441116879319019 | 3.73 | 0.5 | 0.114 |
| TRPS1 | 4 | 9.106349328156054e-07 | 6.596177575914996e-09 | 3.722 | 0.733 | 0.176 |
| FUS | 4 | 1.733040666622057e-05 | 1.8760927378858535e-07 | 3.687 | 0.667 | 0.204 |
| DGKI | 4 | 0.03243892149059269 | 0.001451140163791538 | 3.678 | 0.4 | 0.062 |
| RPS6KA2 | 4 | 0.002873941834358094 | 7.411901252344364e-05 | 3.654 | 0.5 | 0.101 |
| PRKD1 | 4 | 0.0070954322629196535 | 0.00022930394800170178 | 3.623 | 0.467 | 0.085 |
| ZNF618 | 4 | 3.3796314747495136e-05 | 4.169727601577447e-07 | 3.605 | 0.633 | 0.134 |
| SOX5 | 4 | 3.345431361006641e-07 | 2.004328107961825e-09 | 3.604 | 0.767 | 0.178 |
| TIAM2 | 4 | 7.255543243104855e-09 | 2.0791177008021553e-11 | 3.6 | 0.867 | 0.274 |
| REV3L | 4 | 0.0003686652762569988 | 6.632042699520953e-06 | 3.573 | 0.6 | 0.181 |
| KCNMB2 | 4 | 0.09825168297928927 | 0.006397347502750826 | 3.569 | 0.367 | 0.09 |
| ZSWIM6 | 4 | 0.006637273693314906 | 0.0002102710284119504 | 3.556 | 0.467 | 0.083 |
| ANK2 | 4 | 8.532519924374837e-07 | 5.9767711004138e-09 | 3.516 | 0.767 | 0.382 |
| CACNA1E | 4 | 0.006612819275168708 | 0.00020896993172347188 | 3.508 | 0.467 | 0.096 |
| BMPR1B | 4 | 0.05638556657446016 | 0.002989157632618838 | 3.503 | 0.4 | 0.101 |
| AHRR | 4 | 0.0014820278191685452 | 3.373875318651627e-05 | 3.477 | 0.533 | 0.106 |
| CBLB | 4 | 8.429991239346999e-07 | 5.837851132875817e-09 | 3.471 | 0.767 | 0.297 |
| NCOA1 | 4 | 0.025027830058567093 | 0.0010518740962288805 | 3.46 | 0.433 | 0.103 |
| ZC3H3 | 4 | 0.06619335730557707 | 0.003735659502479833 | 3.439 | 0.367 | 0.065 |
| TSPAN4 | 4 | 0.01991533457440457 | 0.0007894481109650144 | 3.428 | 0.433 | 0.103 |
| MAGI2 | 4 | 0.0002456179564510309 | 4.047036295897747e-06 | 3.418 | 0.6 | 0.152 |
| LOC141748211 | 4 | 0.09473567210463617 | 0.006055300859669095 | 3.417 | 0.367 | 0.088 |
| LOC141740893 | 4 | 7.513769952240924e-08 | 3.4090972480914806e-10 | 3.412 | 0.867 | 0.47 |
| KDM6A | 4 | 0.0031990695662600637 | 8.479584220047768e-05 | 3.384 | 0.5 | 0.093 |
| RASA2 | 4 | 0.02229458484861665 | 0.000906832194351915 | 3.383 | 0.433 | 0.106 |
| KLHL29 | 4 | 0.039807385887316145 | 0.001888498128539395 | 3.351 | 0.467 | 0.191 |
| PKD1 | 4 | 0.01691354719643079 | 0.0006367991581558357 | 3.312 | 0.433 | 0.075 |
| SLC24A3 | 4 | 0.001739793824754039 | 4.029929180955388e-05 | 3.284 | 0.533 | 0.134 |
| ASPN | 5 | 0.04099537835031764 | 4.894775732347088e-05 | 3.064 | 0.722 | 0.188 |
| MSX2 | 5 | 0.061748257669264214 | 0.00012370152159976383 | 2.801 | 0.667 | 0.178 |
| LOC141751688 | 5 | 0.02579459709829689 | 1.847897587237698e-05 | 2.573 | 1.0 | 0.749 |
| CXCL14 | 5 | 0.09327612093425545 | 0.0002227400802378145 | 2.462 | 0.833 | 0.632 |
| FXYD6 | 5 | 7.469848311513171e-06 | 5.945911256477889e-10 | 2.442 | 1.0 | 0.749 |
| IGFBP4 | 5 | 0.0024695662831909987 | 3.931491336768286e-07 | 2.329 | 1.0 | 0.599 |
| AIF1L | 5 | 0.05068270886566094 | 8.471996228439701e-05 | 2.291 | 0.778 | 0.333 |
| CYGB | 5 | 0.061748257669264214 | 0.00012779230274622858 | 2.25 | 0.889 | 0.456 |
| FGFR1 | 5 | 0.07849134169179746 | 0.00017493891326676183 | 2.036 | 0.778 | 0.414 |
| SLC6A6 | 5 | 0.016975285521248582 | 5.404850918171959e-06 | 2.01 | 0.944 | 0.744 |
| TIMP3 | 5 | 0.02557816286347554 | 1.6287933050052082e-05 | 1.642 | 1.0 | 0.972 |
| PARD3B | 5 | 0.050412512225640596 | 8.025553168135095e-05 | 1.599 | 0.944 | 0.596 |
| PLA2R1 | 5 | 0.06344748771557265 | 0.00013635932248033601 | 1.568 | 0.889 | 0.581 |
| SYN3 | 5 | 0.050412512225640596 | 7.892722607103725e-05 | 1.513 | 0.944 | 0.516 |
| TGFBI | 5 | 0.02557816286347554 | 1.514950590219574e-05 | 1.472 | 1.0 | 0.855 |
| SAT1 | 5 | 0.055059470317844125 | 0.0001003870773364899 | 1.377 | 0.944 | 0.792 |
| LGALS3 | 5 | 0.050412512225640596 | 7.827089347431742e-05 | 1.2 | 0.944 | 0.902 |
| FSTL1 | 5 | 0.0957400453485182 | 0.00023624463948133918 | 1.178 | 0.944 | 0.805 |
| MMP2 | 5 | 0.061748257669264214 | 0.00012675781860976842 | 1.139 | 1.0 | 0.932 |
| COL1A2 | 5 | 0.055059470317844125 | 0.00010080138639739035 | 1.033 | 1.0 | 0.995 |
| COL6A2 | 5 | 0.08141391557341944 | 0.0001879331012997822 | 0.995 | 1.0 | 0.942 |
| PPDPF | 5 | 0.028584885338163557 | 2.5028555179479353e-05 | 0.947 | 1.0 | 0.992 |
| COL1A1 | 5 | 0.028584885338163557 | 2.373143470991251e-05 | 0.867 | 1.0 | 1.0 |
Saturation
- Left
- The plot shows the Sequencing Saturation metric as a function of downsampled sequencing depth in mean reads per cell, up to the observed sequencing depth. Sequencing Saturation is a measure of the observed library complexity, and approaches 1.0 (100%) when all converted mRNA transcripts have been sequenced. The slope of the curve near the endpoint can be interpreted as an upper bound to the benefit to be gained from increasing the sequencing depth beyond this point.
- Right
- The plot shows the Median Genes per Cell as a function of downsampled sequencing depth in mean reads per cell, up to the observed sequencing depth. The slope of the curve near the endpoint can be interpreted as an upper bound to the benefit to be gained from increasing the sequencing depth beyond this point.