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Sample information : Panthera_tigris



Estimated number of cells
The total number of barcodes identified as cells.
Median UMI counts per cell
Among barcodes identified as cells, the median number of UMI per cell.
Median genes per cell
The median number of genes per cell.
Mean reads per cell
The mean number of reads per cell.
909

Estimated number of cell

19,208

Median UMI counts per cell

3,860

Median genes per cell

47,659

Mean reads per cell

Beads to cells


(left) Beads to cells plot
In library, different mRNAs from the same cell will carry the same cell barcode and random unique molecular identifiers (UMIs). However, some mRNA from dead cells mixed in reflection system was inevitability. This graph shows the distribution of UMI counts in each barcode. Barcodes can be determined to be cell-associated based on their UMI counts or by their expression profiles. Therefore, the graph contains both cell-associated (Colored Regions) and background-associated barcodes (Gray Regions).
(right) Histogram
Histograms of number of beads per droplet.
Summary


Estimated number of cells
The total number of barcodes identified as cells.
Mean reads per cell
The mean number of reads detected per cell.
Mean UMI counts per cell
The average number of UMI counts per cell. This item refers the average abundance of expression in each cell.
Median UMI counts per cell
The median number of UMI counts per cell.
Total genes detected
The total number of genes detected.
Mean genes per cell
The mean number of genes detected per cell. This item refers the activity of gene expression in each cell.
Median genes per cell
The median number of genes detected per cell.
Sequencing saturation
The fraction of UMI originating from an already-observed UMI.
Fraction reads in cells
The fraction of uniquely-mapped-to-transcriptome reads with cell-associated barcode.
Plot
The distribution of nFeature and nCount in violin.
Sample name
Panthera_tigris
Species
Panthera_tigris
Estimated number of cells
909
Mean reads per cell
47,659
Mean UMI count per cell
19,688
Median UMI counts per cell
19,208
Total genes detected
17,355
Mean genes per cell
3,790
Median genes per cell
3,860
Fraction reads in cells
99.73%
Sequencing saturation
42.56%
Sequencing


Number of reads
Number of raw off-machine reads obtained by this sequencing program.
Reads pass QC
Number of reads after quality control (QC) that can be used for downstream analysis. QC includes filtering low quality reads and invalid barcodes.
Reads with exactly matched barcodes
Number of reads with barcodes that match the barcode whitelist before correction.
Reads with failed barcodes
Number of reads with invalid barcodes that fail to match the barcode whitelist.
Reads filtered on low quality
Number of reads in low quality. The QC will filter the average base quality < Q20 or 2 bases < Q10 in the first 15 bases.
Q30 bases in cell barcode
Fraction of cell barcode bases with Q-score ≥ 30.
Q30 bases in UMI
Fraction of UMI bases with Q-score ≥ 30.
Q30 bases in reads
Fraction of RNA read bases with Q-score ≥ 30.
mRNA
Number of reads
51,471,312
Reads pass QC
96.64%
Reads with exactly matched barcodes
100.0%
Reads with failed barcodes
0.0%
Reads filtered on low quality
0.0%
Q30 bases in cell barcode
87.85%
Q30 bases in UMI
87.90%
Q30 bases in reads
93.66%
Droplet index
Number of reads
218,724,924
Reads pass QC
96.18%
Reads with exactly matched barcodes
90.21%
Reads with failed barcodes
3.26%
Reads filtered on low quality
0.56%
Q30 bases in cell barcode
95.89%
Q30 bases in reads
96.75%
Mapping & Annotation


Reads pass QC
Number of reads after quality control (QC) that can be used for downstream analysis. QC includes filtering low quality reads and invalid barcodes.
Reads mapped to genome
Fraction of reads that mapped to the genome.
Plus strand
The number of reads in plus strand.
Minus strand
The number of reads in minus strand.
Mitochondria ratio
The fraction of reads mapped to mitochondria.
Mapping quality corrected reads
The number of reads that align to a single exonic locus but also align to 1 or more non-exonic loci.
Reads mapped to exonic regionse
Fraction of reads that mapped to an exonic region of genome.
Reads mapped to intronic regions
Fraction of reads that mapped to an intronic region of genome.
Reads mapped antisense to gene
Fraction of reads mapped to transcriptome, but on the opposite strand of their annotated gene. This part of reads will be filtered out cause them are opposite to theoretical direction.
Reads mapped to intergenic regions
Fraction of reads that mapped to intergenic regions of genome.
Reads pass QC
49,743,764
Reads mapped to genome
87.46%
Plus strand
57.65%
Minus strand
42.35%
Mitochondria ratio
0.00%
Mapping quality corrected reads
1.0%
Reads mapped to exonic regions
75.7%
Reads mapped to intronic regions
3.6%
Reads mapped antisense to gene
4.7%
Reads mapped to intergenic regions
20.7%
Include introns
True
Cluster


Left
The figure is automated clustering each cell-barcode by UMAP algorithm. The cells clustered into the same group have similar expression profiles. Each dot represents a cell, and is colored according to different cluster.
Right
This plot shows that the total UMI counts for each cell-barcode. Two-dimensional horizontal and vertical coordinates of each dot are obtained using the uniform manifold approximation and projection (UMAP) algorithm. Each dot represents a cell and is colored according to UMI counts. Cells with greater UMI counts likely have higher RNA content than cells with fewer ones.
Marker


Table
The table shows the top 30 differentially expressed genes for each cluster. Here a differential expression test was performed between each cluster and the rest of the sample for each gene. The p-value is a measure of the statistical significance of the expression difference, the smaller the P value, the closer to the theoretical value. Avg_log2FC is log fold-chage of the average expression between the markers for every cluster compared to all remaining cells.
gene cluster p_val_adj p_val avg_log2FC pct.1 pct.2
ANGPTL700.013673021245806740.000400145773499364631.7950.2420.093
LOC12224078000.000103265271266103831.0944086281040527e-061.4880.3650.162
OMD06.220436989284861e-264.2808044795849295e-301.4020.8770.587
GYPC00.031613524884722180.00121833142491531351.320.2380.097
IGFBP300.0011218663713597341.8838028670550897e-051.3070.3730.197
LOC10297080400.00210027431797930054.003688569818087e-051.110.650.523
LOC10297051500.000475757860578324476.580918723848546e-061.10.6350.482
S100A106.322797082719544e-072.9588479913628035e-091.0850.6270.387
IGFBP201.4663383565695486e-168.072883389000336e-201.0721.00.981
ROBO200.00150235102256405922.665631832653319e-051.0570.3650.188
TNNC100.046427157631789580.00199690135020772751.0390.2690.135
TCEA300.0410558278453940360.00168426447793577670.9530.3080.178
FAM43A05.647662708175594e-081.7878500074053907e-100.9520.6810.461
LOC10296074800.0013904524414682552.411355139013146e-050.9510.5270.387
SPP202.2843019748579344e-086.288079209573834e-110.950.7080.485
DCN06.565508531892294e-181.928433552158914e-210.9381.00.965
MAF00.000255652608820902263.149254488950623e-060.930.4920.304
PAQR600.0221147378417664680.00077269434552803040.9280.3420.206
ARHGEF1000.02985771960744830.00110340619566442360.9140.3150.178
F303.410192311984016e-098.213937851451418e-120.910.7960.611
OGN05.36444519228636e-072.3627038215286424e-090.9040.7120.497
HMCN106.565508531892294e-182.2591385767986697e-210.8920.9690.829
ASPN01.3224294135294997e-175.460447650662032e-210.8920.9770.889
LOC10296727801.7858977049608802e-051.4514545540564218e-070.8840.8580.75
PRICKLE200.00060855905521575058.888571679425324e-060.8720.4960.316
EMX200.0553993487592804940.00251624596938442850.8650.2920.161
SLIT200.00038509805871633035.008845440601915e-060.8560.4690.269
SVEP101.2066512786946586e-157.473581658696529e-190.8550.9350.775
ZNF62201.5498285308214584e-068.959162933659246e-090.8540.6380.434
CLU01.3038283889899117e-067.2679168335409025e-090.8490.70.492
LOC10297038700.0112371391447991570.000310875365508861140.8390.3690.216
IL3403.7307500644769715e-062.3620466996895008e-080.8340.6460.434
GAS604.728634585896493e-134.881255164025008e-160.8230.950.798
S1PR301.3791271441168677e-067.877472504418142e-090.7860.7080.499
MED1800.017772868979434170.00056629539174716260.7820.3690.225
NUPR106.189883178027422e-145.963688974770071e-170.7810.9960.945
ADAMTS509.874657210957242e-075.232596553875904e-090.7760.750.57
DISP103.7724949665712064e-106.750042607587321e-130.7650.8850.72
FN3K06.081002417765401e-082.0087269702893074e-100.7560.7730.566
ECM108.475613720275946e-074.424687917268749e-090.7510.6920.465
RETREG107.125568831977707e-082.402813796482745e-100.750.7810.587
SYN100.000111665742460280561.1988064017482463e-060.7390.6620.487
MYO1001.744669667268666e-093.6019606371247667e-120.7360.850.694
PDGFRA01.7858977049608802e-051.4405547426747376e-070.7330.6580.489
ALAS100.013673021245806740.00040084695139451320.7330.4690.328
IL1100.0014020997454872752.4412031904774656e-050.730.5080.347
INAFM101.6264184562939567e-069.513837229714838e-090.7270.7350.561
BTG200.00242804435009132734.795600636406379e-050.7250.50.332
SSC5D00.0055086359376020160.000126459354937133740.7220.4850.323
BDKRB100.0013053849113463332.253299536937868e-050.7190.5770.418
PDLIM214.053246079934612e-075.690332394925751e-091.0840.7390.627
AGPAT210.075880930368135450.0080941051593565440.9710.3570.215
OGFOD210.07095270051733840.007412167048745420.8830.40.278
SOD311.3422458099505842e-052.7341873219005776e-070.8680.80.71
EFCAB1010.0149298991549662010.00104183591928537120.8510.5740.482
INKA110.0148433643083222860.00102966837951888120.7640.5480.435
TWF210.02101788877934320.00159973745715735880.7050.5830.523
VAMP513.0358584922426575e-074.13667319154942e-090.6870.9220.877
LOC10296066910.032974697292755210.00276850393621645840.6760.5740.472
CCN519.241719167914921e-071.411920483983974e-080.6520.9830.945
CLTB16.068057514234825e-086.765021796889695e-100.6350.9650.949
RBP710.00270697146271802950.000125000196234519140.6290.730.645
CRIP112.1522413106556204e-127.257575130557112e-150.6131.00.999
LOC10297128410.049939784981850360.0047496237591987610.6080.5390.439
SSBP410.0006173921729354052.146449743285689e-050.5840.7910.721
SRM10.0370118415134630050.0032067929575011990.5770.6780.583
NAPRT10.060347045083095240.0060425951824309120.5740.6090.557
S100A1611.3422458099505842e-052.7251589861974066e-070.5720.9220.845
CARD1910.0072170201449486910.000409747547972105860.5690.7390.733
TIMP114.5002417872531805e-051.0611539664178216e-060.5681.01.0
NINJ110.00119451548223501364.6610025354569724e-050.5670.8170.76
CLEC3B13.896931067288763e-092.9951846348715335e-110.5631.00.983
HCFC1R115.567109634002621e-112.5669007327656433e-130.5620.9910.988
AVPI110.00053201659185707681.79767494736649e-050.5550.8870.838
PI1610.034109758068577050.0028755387539747360.5540.8430.776
TMED311.4746419457400136e-099.843800752651663e-120.5510.9910.977
RNF18111.3176551657572075e-052.6568919108585904e-070.5370.9220.894
S100A412.498146358212469e-193.094531308776026e-220.5321.01.0
PRAF210.0089050377029208980.00053071107636979550.5320.7910.764
IFI3012.9575650345109146e-056.737003829214182e-070.5260.9220.888
SRA110.00026104289406428487.796615238035896e-060.5250.870.816
ADIRF19.943230600397516e-071.5327807132950544e-080.5191.00.999
MTX110.0091130302962020780.0005456153298256010.5180.7910.758
S100A616.213596358896506e-235.558925928405105e-260.5161.01.0
SHFL10.04215043408757470.00379414822011889770.5150.6350.575
BCL7C15.123252373848846e-051.2481118576439967e-060.5130.9390.87
LOC10297242110.0206933172268871370.0015682213143524770.510.7390.681
DCN10.00184339608317938447.814548119458405e-050.5021.00.972
EIF4EBP310.009351100451471940.0005656608145925150.4940.7650.693
MVB12A10.0471814565895446460.0044227176421143830.4940.7040.652
FAM25A10.09525859975422040.0109674239480290910.4940.7220.655
LOC10297123317.200578160429534e-096.095045858735344e-110.4861.00.985
CGREF110.0141279787592317580.00096934793358709410.4740.8170.742
PPP1CA10.000323111007892293861.0072898394825485e-050.4710.930.878
TNFRSF12A10.0110611649449099960.00071173279357861440.4710.870.814
CCDC85B10.00119237601143246264.6444485752582334e-050.470.8520.843
PHGDH10.0012378234575258384.8597496717356824e-050.4690.9570.863
HIKESHI10.0152241175585769980.00106970091716379570.4680.7650.706
LOC10297035310.072028620581245430.0075741333871132770.4610.6350.591
FXYD111.4848003411505927e-053.096101461486681e-070.460.9740.96
CDC4520.019081476237253540.00068809397483455064.1030.2120.014
CDT124.348697389406264e-121.5562057962227356e-144.0050.4870.045
RRM221.6241796565340144e-294.4709370491611436e-333.9490.7880.158
MCM1020.0318423183655751760.00128631483590892783.8990.2040.015
CDC620.0297094217002141120.0011715297387807233.8670.2040.014
CLSPN24.8979331589356026e-205.730153719765002e-233.7180.6370.076
RMI220.0053434299834231110.000144516412049086963.6420.2390.017
MCM424.159064977429929e-281.717320890825103e-313.4980.770.132
ATAD520.0040532687632500310.000101533950163306823.4280.2480.021
TEDC120.0074791683388288630.00021582067731507853.4030.2390.022
MCM324.516089807857995e-223.729480262493699e-253.3840.690.113
MCM523.8580284894250635e-291.3275165127744352e-323.3460.7880.147
UHRF121.9720554270960608e-354.0714102823537145e-393.2570.8670.259
HELLS21.4216333542210948e-162.5436974199813135e-193.2120.6110.11
MCM624.516089807857995e-223.697075903835997e-253.190.6990.112
CASP220.0067643781501331640.000191326090407042223.1560.2480.03
MCM228.760827357088341e-235.426154167902764e-263.1470.7080.14
MCM727.516263465722586e-401.034514275097734e-433.1360.9120.273
UNG22.433967128139816e-052.7135274568759907e-073.110.3450.047
POLE21.3855190258507121e-096.201623857202204e-123.0670.4690.076
POLE222.8783275711461175e-053.268350762088703e-073.0630.3450.047
PCNA22.959985331444444e-432.037014198227544e-473.0270.9380.39
ORC627.431931453689316e-161.5796319348437102e-183.0250.6020.098
DTL20.032253812398096960.00131181633248057953.0230.2120.023
FANCB20.00040679356776257926.858745034879355e-062.9910.310.051
GINS126.619063503919707e-199.000426566909725e-222.980.6640.121
CENPJ20.0252777714707237940.00097271556276039912.960.230.039
GINS223.69341516217071e-111.4742142963725909e-132.9570.5130.096
PKMYT122.0508613600916697e-118.04480748229476e-142.9460.5130.083
MSH220.0090982863553338120.000276123066733343272.9160.2480.036
DSCC120.00443216711472378240.000114075459425138992.8690.2650.04
TCF1921.6140799927944157e-144.4431353459346657e-172.8640.5930.12
CHAF1A29.792507115680413e-123.639084607024584e-142.8270.5310.09
TK121.3494053521551874e-079.100662343349278e-102.8180.4250.067
DCK20.00072115512213125751.3300500497637947e-052.8080.3010.045
CHEK121.3088502892994206e-078.647004870466201e-102.7850.4250.063
CGAS20.069102354206814280.0036141896082292242.7590.1950.023
RAD51AP122.4843674350924167e-052.7868136530181264e-072.710.3630.067
BRCA220.00017493179842479972.6364368491522356e-062.710.3360.065
CHAF1B20.012498545194860960.000408561624634158462.6750.2480.041
MIS18A20.016175650020927570.00056104381051183642.6320.2390.034
DSN120.000382359387531128046.29037900923253e-062.6210.3190.052
CDCA520.000112635805995828461.5657857553614582e-062.6090.3450.065
MYBL226.619063503919707e-199.110265644373694e-222.5710.7080.168
GINS421.3583357858369115e-172.056526549336732e-202.5510.6810.179
NCAPH20.000154493542540763852.2539832784145575e-062.5260.3450.07
TIMELESS23.393437832339862e-053.9233194951007974e-072.4950.3630.066
SASS620.063580542290829740.00320287364647218852.4690.2120.04
CENPQ21.925041557499595e-052.0666608146028271e-072.4520.3810.083
MNS120.034256006687772540.0014124239266643292.4450.230.045
FABP532.8084996644349356e-311.9327642037264715e-354.7050.8020.181
LOC10295113730.00035517494354952165.792888419326723e-064.2470.2970.037
ITIH537.764118498102296e-151.7098051884885656e-173.7020.5590.081
MASP131.7104153032042689e-125.061445051117168e-153.3240.5230.067
HSD11B130.00122975279555195632.4711844766442173e-053.2330.2880.043
DTX431.4125119151467623e-181.8469290749286687e-213.1750.6490.132
GPX331.993295940828015e-061.7421277577947693e-083.0960.4050.098
RBP431.4243696927050842e-061.2252853319670742e-083.080.4140.103
PPARG30.04145796323402980.00166904607335403182.9080.2160.032
LOC10297016730.0491816832489877160.00205783933764947582.8930.2160.037
ABLIM330.072646657567714390.0034945986951918222.7970.2070.038
CRABP132.0142413704439076e-061.7742956122553175e-082.7110.450.152
LOC10296231839.849404265819311e-086.845983282965731e-102.70.450.1
RSPO130.04800379107331190.0019821261196054742.4680.2340.055
EDIL330.0150486268107451350.00049606305432158282.4090.2520.048
PRKAR2B30.0108534578780575560.00033163136039209652.3260.270.059
CXCL830.00118477504938008482.3563415406430697e-052.2760.3690.137
LOC12223801730.00161408174057482383.365678669012261e-052.2430.3420.111
NDNF30.0041126984452921069.917120345672623e-052.2140.2970.07
IL630.000913935144873741.7044692330932734e-052.210.3690.137
MGST130.000232154550453664523.610689450315063e-062.1730.3690.099
LOC10296120730.0207061545962624780.00072245684263334092.0790.270.069
RGCC31.552829497251417e-115.770614056264298e-142.0560.6310.245
LOC10297051539.012436663303614e-069.303320483762591e-082.0510.6850.505
RARRES235.548248470660543e-317.636430349818378e-352.0440.9910.74
CYGB30.00032315497996645835.15944913304097e-062.0280.3780.129
SFRP130.050736378375898140.0021543146003667442.0120.2520.074
WFDC233.104669043736353e-091.516974069955826e-111.9940.6130.264
PDPN30.0340846826891194540.0012994917218203961.9850.2790.099
SGCA31.309226402679414e-105.315832204121219e-131.9520.6130.238
LOC12223570830.007208014603423090.000194945271429720871.9320.3240.117
LOC10297080436.28425597440905e-057.96633620523639e-071.8880.7030.541
BCL231.4577772051853893e-051.607839614999441e-071.8870.4770.196
FGF735.94071005686608e-112.2894485113515964e-131.8730.6940.341
PDE4B30.00048974623645085258.300329774373599e-061.8580.3960.146
CB3H15orf4830.0462997671117048450.00190220638398512091.8550.2790.103
APOD32.335546136252683e-109.919603478717864e-131.8520.6760.326
FBLN138.45308232743797e-217.562457522310482e-241.8380.910.641
RHOD32.8391707315620343e-053.341120329620177e-071.830.4410.163
VMP131.3990096159454025e-228.664982825344865e-261.8250.9820.857
MLLT330.00181240314767211263.829108570197086e-051.7880.3690.143
RND130.0071429112399319740.000192201382892670961.7880.3420.132
ASS130.00198538420137086644.262885354261306e-051.7710.3870.169
RASA4B30.059893713537940120.0026832845305346511.7440.2880.12
LGMN36.988438401457922e-112.741318483814614e-131.740.6490.284
NFKBIZ30.0078894775571631690.000219891157570097271.7250.3510.152
LOC12223324837.919930757456379e-085.450368699646535e-101.7060.5680.229
PHLDA130.0075567008952782840.000207495950534446051.6960.3510.154
CACNA2D130.00728593588325105860.000198055513996570661.6080.3690.174
EDNRB31.553530807007451e-061.3470847270176782e-081.6030.550.243
CRIP340.03653083679978650.00072733341024833353.1260.2330.027
CENPA46.232553402576819e-118.149371319865085e-142.3470.5920.14
DNPH141.4420520137885483e-195.954381723715704e-232.1170.8540.353
PRRG441.6531459735410624e-056.826010488780108e-081.9050.4850.186
LOC10295592540.028654903260754740.00052849178128706011.8150.3110.106
CENPW47.577452114849992e-111.1472296918773643e-131.8070.7090.344
CDKN340.096281183765039570.00264374043921621281.6960.2720.091
DUT41.0507332346550393e-146.507879094277994e-181.6520.8540.624
LOC12223275541.8811471073069714e-291.2945751202993403e-331.5530.990.971
PTTG140.0096421851039148930.000124085652359237851.5520.3880.166
POC1A47.186931055512952e-053.462151083104443e-071.470.5150.228
CDCA340.0001029843089932275.173666338521486e-071.450.4850.173
CRABP244.02387465931219e-098.030580491366975e-121.350.8160.628
BIRC543.1468795841112183e-061.1694411777717004e-081.2810.660.371
HMGB145.560779825505682e-201.9134195256712138e-231.2010.990.946
UCP240.0197737811341057030.00031978794071397981.1960.4170.231
SUN240.063707678159174290.00150380108792215151.170.3880.219
HNRNPH140.00109930429146683288.700692247173244e-061.1330.6210.469
SPC2446.046391239782823e-134.993234799903233e-161.1260.9130.844
HNRNPA2B143.061119031113529e-236.319838341023046e-271.0931.00.974
PI4KA42.33023789245614e-076.574891858144776e-101.0770.9420.875
FUS44.534205322802389e-115.6166606434824165e-141.0650.9030.783
PCGF340.0382368839520285460.00077100041276886411.0570.4270.239
TRDMT140.08465618705071150.0021942211148004181.0410.4270.274
TRAPPC6A40.0159941172956336660.0002432523516850211.0210.4760.279
HNRNPM41.473364200009591e-131.1153400454273966e-161.020.9420.819
ZEB146.111088575969479e-062.3551094918057312e-081.0120.7570.577
CLEC11A40.00141025947656334681.2616731949159388e-050.9730.6120.389
CE3H16orf8240.00040768779545858692.4689646961912908e-060.970.8640.8
PTMS44.9380442481195874e-211.3593129855122393e-240.9581.00.982
PJA140.0053635540154065485.905778284117044e-050.9540.5630.357
HMGN241.0235475880031869e-111.0565834299117613e-140.9430.9610.943
NUCKS145.16359998897025e-172.4874544025044218e-200.9161.00.982
ARHGAP1046.5982991239108e-072.0433793997383936e-090.8950.8930.779
RNF15240.0100607134382932060.000133625882154744250.8650.5920.423
CDK640.080814937164627340.00205221332418604970.8610.5240.383
SRSF241.838349901827747e-074.807466538397521e-100.8550.9030.772
LMNA47.122044742966055e-299.802552808431705e-330.8461.00.999
IGFBP243.896002646283292e-051.7427580483684123e-070.8331.00.985
CASP840.039715531708074610.00080628187006276310.830.5630.423
TRIO40.0075440571583801989.115256633863526e-050.820.660.495
DTYMK40.00243596608037390972.397241411420199e-050.8150.7090.47
CD7040.061709174570541380.0014184064624981640.8070.5920.436
PTGIS40.0061720341537311647.093315695224723e-050.8010.6410.444
DPT40.0338396282210704160.00066137598339990350.80.6310.539
XRN240.076961170603545350.0019172764268449120.7990.5340.394
MICAL240.006165701858756696.958744097695253e-050.7980.7180.545
RRBP142.010810400081142e-103.182756809707953e-130.7960.990.958
HNRNPR40.014472562447382740.000211147425424619160.7950.6310.557
PTMA46.28045397330088e-144.3221072006750255e-170.7851.00.996
SYNPO256.584971551302097e-059.153976005526278e-077.2170.3470.004
BAMBI50.0107324607809719060.00033827451914425256.7480.2530.003
EVA1C50.092389647201424560.0047558637469317726.3390.20.003
LOC12223939353.8002315307124526e-054.864380047570822e-076.0840.360.01
IL3350.060659053371575230.0027342701781282625.9450.2130.004
TINAGL150.0074523336878959030.00021334875880288325.9150.2670.009
EBF250.0018915730186796894.269740211457835e-055.9140.2930.008
GNAI150.036710088781157820.00151579748597444735.8930.2270.005
BST150.0021075466936308574.945794663327522e-055.7420.2930.012
IRAG150.00048401351636629128.793583946094616e-065.6290.320.01
ARHGDIB51.4346146265733476e-071.2044799700085913e-095.4590.440.02
MGST250.008215258438499690.000241409080809260725.1760.2670.012
LPL51.8425666813346053e-091.0271000012944948e-114.6410.5070.037
FOXS152.630977689065365e-072.3537753738799634e-094.6020.440.024
LMOD154.987724867051149e-093.1235493971623055e-114.2860.5070.056
FMOD56.445093219920847e-084.878950204330694e-104.250.480.06
ACTA255.2435721477027485e-233.247687655999225e-264.0640.920.664
IGFBP754.183932089258305e-302.8793146302789243e-343.5770.960.411
COL4A154.2548899587692085e-295.856293384858865e-333.5630.960.563
LGALS954.920219953230465e-065.38735565121536e-083.3650.440.072
SHOX253.409355975018606e-063.5897836637385367e-083.3070.440.059
COL8A150.000112417841902176421.6478563295825188e-063.2870.40.079
APOE50.00200536024874704454.623189617578005e-053.2870.320.035
COL4A253.922279698244546e-193.2390996062854965e-223.2540.80.236
NOTCH350.098464267248104670.0051634279569923453.2540.2270.031
PPP1R14A50.000113293700746004151.668491635788651e-063.1920.40.079
COTL151.033560355023429e-171.0669193672391049e-203.1490.760.219
TRPV250.0376808731960443160.00157144099902297563.130.2530.033
PDGFD50.00128298896194676372.6664556225168444e-053.0970.3470.058
STBD150.0053142819524929140.000144825246245075643.0380.3070.042
ARRB153.981918457405213e-064.302258604449924e-083.0040.4530.077
TST53.2724623381761715e-072.950193147760502e-093.0020.4930.088
SHROOM350.00115506977332832472.384701204311454e-052.9610.3470.056
NR2F254.3766324488891084e-166.927434197539708e-192.780.7470.26
TMTC150.074985847914433760.00364324606892782542.7590.240.037
MLANA50.000395531825474574246.995504724173531e-062.7560.3870.075
SPECC150.00193302871571102244.4431325514244135e-052.7530.3470.069
TNS350.00087026713420779691.7149865296266867e-052.6920.3870.101
LOC10297180853.836597867258158e-064.0660386178360494e-082.6140.5330.199
NR2F152.2351422785401947e-127.690944458537591e-152.6030.6930.24
IGFBP550.0305208631117075170.00120982844624207072.5920.320.107
VCAM150.00307213116023872347.764005031224818e-052.4730.3730.107
ZDHHC255.164275299033942e-093.2696533446502145e-112.4320.60.186
ADAMTS451.0998357043479621e-051.2622825969457805e-072.3790.4930.143
NES53.074513902726525e-091.7984562778319085e-112.3420.6670.323
AKAP1250.031719832027568650.00126934556172769982.3250.2930.075
THY150.00054128286357833531.0057557853289557e-052.3120.40.089
PALLD52.8561197353572205e-082.063812347481303e-102.3020.6270.232
SFRP150.0083007795765182790.00024449340435963272.2920.3330.075
COL18A152.704993481282995e-231.3030730417026335e-262.2810.9470.637
CDC2065.5071867402683735e-317.579914307712302e-356.2210.9520.085
KIFC162.2019184955555527e-101.7426235426941614e-125.9840.5480.012
NEK263.869254076480076e-093.381703032915627e-115.9690.5160.013
KIF2362.907313213415944e-282.200842705083985e-315.8290.9030.049
ASPM64.876886979277082e-293.02057551534607e-325.7640.9190.093
CCNB168.485173042875638e-271.0510846794560696e-295.5840.8870.064
KIF2C60.00068863339060618151.583676544406208e-055.5230.3390.012
PLK161.0877101170340805e-155.464375372891602e-185.5080.6770.031
DLGAP567.107569304698409e-241.2717418066351844e-265.4820.8390.051
CENPF67.00654894855402e-202.266243208189656e-225.4720.7740.063
CCNB265.40365702577822e-182.119664513587217e-205.4440.7260.041
CCNB368.835347181674005e-135.593916046479998e-155.4150.6130.03
CENPL60.000125770193821759082.371552756669327e-065.4020.3710.013
TTK61.9592671964148e-053.168589850371468e-075.3920.4030.018
PIMREG63.5821167594238096e-228.135011565686169e-255.3770.8060.05
CCNA264.61081140968795e-221.0788492734800792e-245.360.8060.045
MKI6762.3180197944436043e-291.276179089914585e-325.2860.9350.06
KIF1164.187540519073809e-211.0950831995375729e-235.2340.790.049
CDC25C62.8190042259726945e-073.453188027136051e-095.1640.4680.024
PRC162.1780996270840183e-291.0492531408428964e-325.0950.9350.09
CDCA365.5071867402683735e-315.209311613439015e-355.0860.9680.151
NUSAP163.900655933729173e-142.201182207458483e-165.0790.6610.064
DEPDC162.3074180271071768e-063.318769304696167e-085.070.4350.015
CDCA861.4387104742286247e-175.841574425675373e-205.0450.7260.041
KIF1562.545168142305646e-054.2037048665154157e-075.0240.4030.019
AURKB65.839940718187121e-133.657247301321506e-154.9360.6290.048
KIF20A66.858706406572636e-202.1712235544858665e-224.9260.7740.046
PTTG161.1355136601004271e-294.688653197028809e-334.9030.9520.133
TOP2A61.2858404330222692e-165.751815301524155e-194.8780.7260.098
CENPE61.018789306727868e-126.520363741359282e-154.8480.6290.051
RACGAP160.00016281590976329773.1821428926279366e-064.8380.3710.017
NUF262.0025948316707882e-224.410091157763762e-254.8140.8230.076
KNSTRN68.827998652396243e-164.19194760866658e-184.8140.6940.054
PBK64.2678194501157e-274.99297575197625e-304.7860.9030.071
TACC361.2649480065326788e-165.571307715786349e-194.7670.710.058
SHCBP164.99473105813475e-113.746649819948302e-134.7280.5810.033
CKAP2L61.3970982897019348e-081.394117762072676e-104.6810.5160.032
LOC10295139961.1028036960417013e-251.66964980475655e-284.680.8870.103
CDCA261.7424330895730912e-062.43420216904093e-084.6480.4520.031
AURKA62.1831973355745323e-215.559032510925449e-244.6220.8060.076
CEP5562.986304235378058e-121.9934726504140915e-144.6170.6130.033
GTSE166.3032265920366336e-211.8218671589397743e-234.6050.8060.068
MXD368.782926229546413e-051.6017310927188764e-064.6010.3870.024
NDC8063.952340637739665e-093.5087188924947826e-114.5690.5320.032
SPAG561.2852179643975427e-052.0342724644651768e-074.5240.4190.027
MTFR263.114845756779281e-102.5294322434791488e-124.5170.5650.039
IQGAP361.6042120478777683e-091.3579112372786837e-114.5130.5480.04
TPX269.004130378005417e-303.0577068020676394e-334.4890.9520.176
CKAP261.5463042245584718e-223.298839099945814e-254.4850.8390.125
ANLN62.3853668829215017e-234.432241816728411e-264.4660.8550.115
Saturation


Left
The plot shows the Sequencing Saturation metric as a function of downsampled sequencing depth in mean reads per cell, up to the observed sequencing depth. Sequencing Saturation is a measure of the observed library complexity, and approaches 1.0 (100%) when all converted mRNA transcripts have been sequenced. The slope of the curve near the endpoint can be interpreted as an upper bound to the benefit to be gained from increasing the sequencing depth beyond this point.
Right
The plot shows the Median Genes per Cell as a function of downsampled sequencing depth in mean reads per cell, up to the observed sequencing depth. The slope of the curve near the endpoint can be interpreted as an upper bound to the benefit to be gained from increasing the sequencing depth beyond this point.