## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[/beegfs/scratch/ws/ws1/torgunakov-worksep/Alignment/Oncobox_rna/PrC_14/PrC_14_paired.sorted.bam] OUTPUT=/beegfs/scratch/ws/ws1/torgunakov-worksep/Alignment/Oncobox_rna/PrC_14/PrC_14_paired.sorted.markdup.bam METRICS_FILE=/beegfs/scratch/ws/ws1/torgunakov-worksep/Alignment/Oncobox_rna/PrC_14/PrC_14_dedup_metrics.txt CREATE_INDEX=true MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false FLOW_MODE=false FLOW_QUALITY_SUM_STRATEGY=false USE_END_IN_UNPAIRED_READS=false USE_UNPAIRED_CLIPPED_END=false UNPAIRED_END_UNCERTAINTY=0 FLOW_SKIP_FIRST_N_FLOWS=0 FLOW_Q_IS_KNOWN_END=false FLOW_EFFECTIVE_QUALITY_THRESHOLD=15 ADD_PG_TAG_TO_READS=true REMOVE_DUPLICATES=false ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Thu Oct 09 18:07:13 NOVT 2025 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 176208 32781117 607435 325806 96124 2463646 0 0.076415 207022043 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets non_optical_sets 1.0 1 29112637 29112637 2.0 1.853554 951604 951604 3.0 2.582109 110458 110458 4.0 3.203971 45152 45152 5.0 3.734763 25438 25438 6.0 4.187824 16118 16118 7.0 4.574535 10875 10875 8.0 4.904615 7854 7854 9.0 5.186355 5782 5782 10.0 5.426836 4396 4396 11.0 5.6321 3552 3552 12.0 5.807304 2767 2767 13.0 5.956849 2314 2314 14.0 6.084495 1868 1868 15.0 6.193447 1609 1609 16.0 6.286444 1409 1409 17.0 6.365822 1191 1191 18.0 6.433575 1031 1031 19.0 6.491406 812 812 20.0 6.540768 747 747 21.0 6.582901 656 656 22.0 6.618864 610 610 23.0 6.649561 557 557 24.0 6.675762 492 492 25.0 6.698126 491 491 26.0 6.717214 407 407 27.0 6.733508 331 331 28.0 6.747415 305 305 29.0 6.759286 280 280 30.0 6.769418 262 262 31.0 6.778066 242 242 32.0 6.785448 243 243 33.0 6.791749 226 226 34.0 6.797127 176 176 35.0 6.801718 202 202 36.0 6.805636 173 173 37.0 6.80898 172 172 38.0 6.811835 140 140 39.0 6.814272 144 144 40.0 6.816351 143 143 41.0 6.818127 117 117 42.0 6.819642 137 137 43.0 6.820935 120 120 44.0 6.822039 100 100 45.0 6.822981 100 100 46.0 6.823786 108 108 47.0 6.824472 86 86 48.0 6.825058 90 90 49.0 6.825558 83 83 50.0 6.825985 83 83 51.0 6.82635 76 76 52.0 6.826661 68 68 53.0 6.826926 56 56 54.0 6.827153 71 71 55.0 6.827346 43 43 56.0 6.827511 60 60 57.0 6.827652 52 52 58.0 6.827772 75 75 59.0 6.827875 61 61 60.0 6.827963 55 55 61.0 6.828037 48 48 62.0 6.828101 48 48 63.0 6.828156 42 42 64.0 6.828202 46 46 65.0 6.828242 37 37 66.0 6.828276 46 46 67.0 6.828305 40 40 68.0 6.828329 36 36 69.0 6.828351 33 33 70.0 6.828369 37 37 71.0 6.828384 36 36 72.0 6.828397 34 34 73.0 6.828408 19 19 74.0 6.828418 28 28 75.0 6.828426 23 23 76.0 6.828433 19 19 77.0 6.828439 29 29 78.0 6.828444 30 30 79.0 6.828448 28 28 80.0 6.828452 16 16 81.0 6.828455 23 23 82.0 6.828458 33 33 83.0 6.82846 12 12 84.0 6.828462 11 11 85.0 6.828464 21 21 86.0 6.828465 23 23 87.0 6.828466 18 18 88.0 6.828467 17 17 89.0 6.828468 11 11 90.0 6.828469 17 17 91.0 6.82847 25 25 92.0 6.82847 15 15 93.0 6.828471 11 11 94.0 6.828471 13 13 95.0 6.828471 9 9 96.0 6.828472 16 16 97.0 6.828472 17 17 98.0 6.828472 14 14 99.0 6.828472 13 13 100.0 6.828472 18 18 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182.0 0 4 4 183.0 0 6 6 184.0 0 4 4 185.0 0 3 3 186.0 0 5 5 187.0 0 5 5 188.0 0 3 3 189.0 0 4 4 190.0 0 2 2 191.0 0 6 6 192.0 0 2 2 194.0 0 3 3 195.0 0 2 2 196.0 0 2 2 198.0 0 3 3 199.0 0 2 2 200.0 0 2 2 201.0 0 1 1 202.0 0 3 3 203.0 0 5 5 204.0 0 2 2 205.0 0 4 4 207.0 0 6 6 208.0 0 2 2 209.0 0 2 2 210.0 0 2 2 211.0 0 2 2 212.0 0 1 1 213.0 0 1 1 214.0 0 2 2 215.0 0 2 2 216.0 0 1 1 217.0 0 3 3 218.0 0 3 3 219.0 0 2 2 220.0 0 2 2 221.0 0 1 1 222.0 0 2 2 223.0 0 2 2 224.0 0 1 1 225.0 0 2 2 226.0 0 3 3 227.0 0 4 4 228.0 0 2 2 229.0 0 1 1 230.0 0 3 3 231.0 0 2 2 232.0 0 1 1 233.0 0 2 2 234.0 0 2 2 238.0 0 2 2 239.0 0 4 4 240.0 0 2 2 241.0 0 2 2 242.0 0 3 3 243.0 0 1 1 244.0 0 2 2 245.0 0 4 4 246.0 0 2 2 249.0 0 1 1 250.0 0 2 2 251.0 0 1 1 252.0 0 1 1 253.0 0 1 1 254.0 0 3 3 256.0 0 1 1 257.0 0 1 1 258.0 0 2 2 260.0 0 1 1 262.0 0 1 1 263.0 0 1 1 264.0 0 2 2 265.0 0 1 1 268.0 0 1 1 270.0 0 2 2 274.0 0 2 2 275.0 0 1 1 276.0 0 1 1 278.0 0 1 1 279.0 0 1 1 280.0 0 4 4 283.0 0 1 1 284.0 0 2 2 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