AqmcR2.chr1.chr2.fish.bed - this files contain genome coordinates of putative region for fish in chr1 AqmcR2.chr1.chr2.fish.fa - this files contain sequences of putative region for fish in chr1 For example, we will make probe to distinguish 3L1 and 3L2 chromosome haplotypes. To make probe on _3L1_: 1) AqmcR2.12.full.agp AqmcR2.12.full.contig.breaks.bed -- to define of contigs that you will test 2) AqmcR2.3L1.3L2.fish.bed -- to find "good" locus in tested contigs 3) AqmcR2.3L1.3L2.fish.fa -- to define of nucleotide sequence of choosen "good" locus To make probe on _3L2_: 1) AqmcR2.12.full.agp AqmcR2.12.full.contig.breaks.bed -- to define of contigs that you will test 2) AqmcR2.3L2.3L1.fish.bed -- to find "good" locus in tested contigs 3) AqmcR2.3L2.3L1.fish.fa -- to define of nucleotide sequence of choosen "good" locus