LOCUS       pCAGGS-mCherry_(#41583) 5488 bp ds-DNA     circular     11-OCT-2024
DEFINITION  .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported using the Genbank importer. File name:
            addgene-plasmid-41583-sequence-174272.gbk synthetic circular DNA
FEATURES             Location/Qualifiers
     rep_origin      complement(142..730)
                     /label="ori"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     complement(222..241)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(901..1761)
                     /label="AmpR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     1524..1543
                     /label="Amp-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     promoter        complement(1762..1866)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     enhancer        1897..2276
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     promoter        2278..2555
                     /label="chicken beta-actin promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     intron          2556..3572
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     3496..3518
                     /label="pCAGGS-5"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     3580..3599
                     /label="pCAG-F"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     regulatory      3631..3640
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             3637..4344
                     /label="mCherry"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             3637..4344
                     /label="Translation 3637-4344"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK"
     primer_bind     complement(3782..3800)
                     /label="mCherry-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     4050..4069
                     /label="mCherry-F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4323..4412
                     /label="new olig"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    4325..4344
                     /label="5H olig "
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    4345..4388
                     /label="barc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4364..4369
                     /label="R"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    4389..4409
                     /label="3H olig"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(4389..4412)
                     /label="Rev"
                     /note="sequence: agggaaaaagatcgagatctgagt"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     polyA_site      4467..4522
                     /label="-beta-globin poly(A) signal"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     complement(4521..4540)
                     /label="rbglobpA-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          4832..4854
                     /label="PCR-RIGS-BamHI-F"
                     /note="sequence: cttctcttatggagatccctcga"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     complement(4883..4899)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     complement(4883..4899)
                     /label="M13 Reverse"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(4896..4918)
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     protein_bind    4907..4923
                     /label="lac operator"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        complement(4931..4961)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    4976..4997
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     promoter        5056..5251
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     rep_origin      5102..5237
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     5164..5183
                     /label="SV40pro-F"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     polyA_signal    5257..5391
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     complement(5294..5313)
                     /label="SV40pA-R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     5348..5367
                     /label="EBV-rev"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(5459..5476)
                     /label="L4440"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
ORIGIN
        1 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
       61 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
      121 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
      181 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
      241 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
      301 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
      361 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
      421 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
      481 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
      541 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
      601 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
      661 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
      721 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
      781 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
      841 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
      901 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
      961 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     1021 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     1081 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     1141 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     1201 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     1261 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     1321 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     1381 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     1441 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     1501 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     1561 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     1621 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     1681 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     1741 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     1801 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     1861 tccgcgcaca tttccccgaa aagtgccacc tgggtcgaca ttgattattg actagttatt
     1921 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     1981 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     2041 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     2101 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     2161 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     2221 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg
     2281 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
     2341 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc
     2401 gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg
     2461 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc
     2521 ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc
     2581 ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac
     2641 tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt
     2701 aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc
     2761 ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg
     2821 cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg
     2881 cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg
     2941 gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg
     3001 tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg
     3061 gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg
     3121 gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct
     3181 cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca
     3241 gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct
     3301 gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc
     3361 ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc
     3421 gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg
     3481 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta
     3541 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc
     3601 tgtctcatca ttttggcaaa gaattctagc gccaccatgg tgagcaaggg cgaggaggat
     3661 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
     3721 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     3781 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
     3841 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
     3901 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     3961 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
     4021 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
     4081 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
     4141 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
     4201 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
     4261 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
     4321 ggcggcatgg acgagctgta caagTGAVAV AGDGTBTCHC TBTGGTACCG DGAVACHCTB
     4381 TGDGAVAVac tcagatctcg atctttttcc ctctgccaaa aattatgggg acatcatgaa
     4441 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
     4501 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
     4561 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa
     4621 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
     4681 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt
     4741 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct
     4801 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca
     4861 gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     4921 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     4981 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     5041 cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     5101 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     5161 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     5221 aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat
     5281 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5341 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt
     5401 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     5461 cgctcactga ctcgctgcgc tcggtcgt
//