FastQCFastQC Report
Wed 8 Sep 2021
G1_S5_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG1_S5_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16619
Sequences flagged as poor quality0
Sequence length245-251
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCAGGAATTGTGAAAAAGTGAGTNTAAATGTATTTGGCTAAGGTGTA674640.59209338708707No Hit
ATGTCAGGAATTGTGAAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTA483629.09922378001083No Hit
ATTTCAGGAATTGTGAAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTA4802.888260424814971No Hit
ATATCAGGAATTGTGAAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTA4312.5934171731151094No Hit
ATGTCAGGAATTGTGAAAAGTGAGTTNAAATGTATTTGGCTAAGGTGTAT2551.5343883506829532No Hit
ATGTCAGGAATTGTGAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTAT1801.083097659305614No Hit
ATGTCAGGAATGTGAAAAAGTGAGTTNAAATGTATTTGGCTAAGGTGTAT1210.7280823154221072No Hit
ATCTCAGGAATTGTGAAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTA1090.6558758048017329No Hit
ATGTCAGGATTGTGAAAAAGTGAGTTNAAATGTATTTGGCTAAGGTGTAT980.5896865033997233No Hit
ATGTCAGGAATGTGAAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTAT880.529514411216078No Hit
ATGTCAGGATTGTGAAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTAT660.3971358084120585No Hit
ATGTCAGGAATTTGAAAAAGTGAGTTNAAATGTATTTGGCTAAGGTGTAT590.35501534388350686No Hit
ATGTCAGGAATTGTAAAAAGTGAGTTNAAATGTATTTGGCTAAGGTGTAT550.33094650701004874No Hit
ATGTCAGAATTGTGAAAAAGTGAGTTNAAATGTATTTGGCTAAGGTGTAT530.3189120885733197No Hit
ATGTCAGGAATTGTGAAAAAGTGAGTNTAAATGTATTTGGCTAAGGCGTA410.2467055779529454No Hit
ATGTCAGGAATTTGAAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTAT380.22865395029785185No Hit
ATGTCAGGAATTGTGAAAAAGTGAGTTTAAATGTATTTGGCTAAGGCGTA380.22865395029785185No Hit
ATGTCAGGAATTGTGAAAAAGTGAGTNTAAATGTATCTGGCTAAGGTGTA360.21661953186112282No Hit
ATGTCAGGAATTGGAAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTAT360.21661953186112282No Hit
ATGTCAGGAATTGTGAAAAAGTGAGTNTAAATGTATTCGGCTAAGGTGTA350.21060232264275827No Hit
ATGGTCAGGAATTGTGAAAAAGTGAGNTTAAATGTATTTGGCTAAGGTGT310.1865334857693002No Hit
ATGTCAGGAATTGGAAAAAGTGAGTTNAAATGTATTTGGCTAAGGTGTAT310.1865334857693002No Hit
ATGTCAGGAATTGTGAAAAAGTGAGTNTAAATGTACTTGGCTAAGGTGTA300.1805162765509357No Hit
ATGTCAGGAATTGTGAAAAAGTGAGTNTAGATGTATTTGGCTAAGGTGTA290.17449906733257114No Hit
ATGTTAGGAATTGTGAAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTA290.17449906733257114No Hit
ATGTTAGGAATTGTGAAAAAGTGAGTNTAAATGTATTTGGCTAAGGTGTA280.16848185811420663No Hit
ATGTCAGGAATTGTAAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTAT270.1624646488958421No Hit
ATGGTCAGGAATTGTGAAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGT260.1564474396774776No Hit
ATGTCAGGAATTGTGAAAAAGTGAGTNTAAATGTATTTGGCTAGGGTGTA250.15043023045911305No Hit
ATGTCAGGAATTGTGAAAAAGTGAGTNTAAATGCATTTGGCTAAGGTGTA230.13839581202238402No Hit
TGTCAGGAATTGTGAAAAAGTGAGTTNAAATGTATTTGGCTAAGGTGTAT230.13839581202238402No Hit
ATGTCAGGAATTGTGAAAAAGTGAGTNTAAACGTATTTGGCTAAGGTGTA230.13839581202238402No Hit
ATGTCAGAATTGTGAAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTAT220.1323786028040195No Hit
ATGTCAGGAATTGTGAAAAAGTGAGTNTAAATGTATTTGGCCAAGGTGTA220.1323786028040195No Hit
ATGCCAGGAATTGTGAAAAAGTGAGTNTAAATGTATTTGGCTAAGGTGTA220.1323786028040195No Hit
ATGCAGGAATTGTGAAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTAT210.12636139358565499No Hit
ATGTCAGGAATTGTGAAAAAGTGAGTTTAAATGTATCTGGCTAAGGTGTA210.12636139358565499No Hit
ATTCAGGAATTGTGAAAAAGTGAGTTNAAATGTATTTGGCTAAGGTGTAT210.12636139358565499No Hit
ATGTCAGGAATTGTGAAAAAGTGAGCTTAAATGTATTTGGCTAAGGTGTA210.12636139358565499No Hit
ATGTCAGGAATTGTGAAAAAGTGAGTCTAAATGTATTTGGCTAAGGTGTA200.12034418436729045No Hit
ATGTCAGGAATTGTGAAAAAGTGAGTTTAAATGTATTCGGCTAAGGTGTA200.12034418436729045No Hit
ATGTCAGGAATTGTGAAAAAGTGAGTTTAAATGTACTTGGCTAAGGTGTA200.12034418436729045No Hit
ATTCAGGAATTGTGAAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTAT190.11432697514892592No Hit
ATGTAGGAATTGTGAAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTAT190.11432697514892592No Hit
ATATCAGGAATTGTGAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTAT190.11432697514892592No Hit
ATGTCAGGAATTGTGAAAAAGTGAGTTTAAACGTATTTGGCTAAGGTGTA190.11432697514892592No Hit
ATTTCAGGAATTGTGAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTAT190.11432697514892592No Hit
ATGTCAGGAATTGTGAAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTG170.10229255671219688No Hit
ATGTCAGGAATTGTGAAAAAGTGAGCNTAAATGTATTTGGCTAAGGTGTA170.10229255671219688No Hit
ATGTCAGGAATTGTGAAAAAGTGAGTTTAAATGTATTTGGCTAGGGTGTA170.10229255671219688No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)