FastQCFastQC Report
Wed 13 Oct 2021
cutIL_52_S52_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamecutIL_52_S52_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10146
Sequences flagged as poor quality0
Sequence length43-151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCAGGAATTGTGAAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTA210420.73723634930022No Hit
ATGTCAGGAATTGTGAAAAAGTGAGGGAAAGAAGTTGGAGTCATTAAAACTCGTTT155215.296668637886851No Hit
ATGTCAGGAATTGTGAAAAAGTGAGGGAAAGAAAGTTGGAGTCATTAAAACTCGTTT133013.108614232209737No Hit
ATGTCAGGAATTGTGAAAAAGTGAGAGAAAGAAGTTGGAGTCATTAAAACTCGTTT6146.0516459688547215No Hit
ATGTCAGGAATTGTGAAAAAGTGAGTTTACATACACTTAAGTTGGAGTCATTAAAACTCGTTT4404.336684407648335No Hit
ATGTCAGGAATTGTGAAAAAGTGAGTTTAAATGTATTCGGCTAAGGTGTA2602.5625862408831064No Hit
ATGTCAGGAATTGTGAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTAT790.7786319731914054No Hit
ATGTCAGGAATTGTGAAAAAGTGAGTCTAAATGTATTTGGCTAAGGTGTA770.7589197713384584No Hit
ATGTCAGGAATTGTGAAAAAGTGAGGGAAAGAAGTTGGAGTCATTAATACTCGTTT660.6505026611472502No Hit
ATGTCAGGAATTGTGAAAAAGTGAGTTTAAGTGTATTTGGCTAAGGTGTA620.6110782574413562No Hit
ATGTCAGGAATTGTGAAAAGTGAGGGAAAGAAGTTGGAGTCATTAAAACTCGTTT590.5815099546619357No Hit
ATGTCAGGAATTGTGAAAAGTGAGGGAAAGAAAGTTGGAGTCATTAAAACTCGTTT480.4730928444707274No Hit
ATGTCAGGAATTGTGAAAAAGTGAGGGAAAGAAAGTTGGAGTCATTAAACTCGTTT390.384387936132466No Hit
ATGTCAGGAATTGTGAAAAAGTGAGGGAAAGAAGTTGGAGTCATTAAACTCGTTT380.37453183520599254No Hit
ATGTCAGGATTGTGAAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTAT350.3449635324265721No Hit
ATGTCAGGAATTGTGAAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTG310.3055391287206781No Hit
ATGTCAGGAATGTGAAAAAGTGAGGGAAAGAAAGTTGGAGTCATTAAAACTCGTTT290.28582692686773115No Hit
ATGTCAGGAATGTGAAAAAGTGAGGGAAAGAAGTTGGAGTCATTAAAACTCGTTT260.2562586240883107No Hit
ATGTCAGGAATTGTGAAAAAGTGAGAGAAAGAAGTTGGAGTCATTAATACTCGTTT260.2562586240883107No Hit
ATGTCAGGATTGTGAAAAAGTGAGGGAAAGAAGTTGGAGTCATTAAAACTCGTTT250.2464025231618372No Hit
ATGTCAGGAATTGTGAAAAAGTGAGGGAAAGAAGTTGGAGTCATTAAAACTCGTT250.2464025231618372No Hit
ATGTCAGGAATTGTGAAAAAGTGAGGGAAAGAAGTTGGAGTCATAAAACTCGTTT240.2365464222353637No Hit
ATGTCAGGAATGTGAAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTAT210.20697811945594324No Hit
ATGTCAGGAATTGTGAAAAAGTGAGGGAAAGAAAGTTGGAGTCATTAAAACTCGTT210.20697811945594324No Hit
ATGTCAGGAATTGTGAAAAAGTGAGAGAAAGAAGTTGGAGTCATTAAACTCGTTT200.19712201852946973No Hit
ATGTAAGGAATTGTGAAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTA200.19712201852946973No Hit
ATGTCAGGAATTGTGAAAAAGTGAGGGAAAGAAAGTTGGAGTCATTATAACTCGTTT200.19712201852946973No Hit
ATGTCAGGAATTGTGAAAAAGTGAGGGAAAGAAAGTTGGAGTCATAAAACTCGTTT200.19712201852946973No Hit
ATGTCAGGAATTGTGAAAAAGTGAGGGAAAGAAAGTTGGAGTAATTAAAACTCGTTT190.18726591760299627No Hit
ATGTCAGGAATTGTGAAAAAGTGAGGGAAAGAAGTTGGAGTCATTAAAACTAGTTT180.17740981667652278No Hit
ATGTCAGGAATTGTGAAAAAGTGAGGGAAAGAAAGTTGGATCATTAAAACTCGTTT180.17740981667652278No Hit
ATGTCAGGAATTGTGAAAAGTGAGAGAAAGAAGTTGGAGTCATTAAAACTCGTTT170.1675537157500493No Hit
ATGTCAGGAATTTGAAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTAT170.1675537157500493No Hit
ATGTCAGGATTGTGAAAAAGTGAGGGAAAGAAAGTTGGAGTCATTAAAACTCGTTT170.1675537157500493No Hit
ATGTCAGGAATTGTGAAAAAGTGAGGGGAAGAAGTTGGAGTCATTAAAACTCGTTT160.1576976148235758No Hit
ATGTAAGGAATTGTGAAAAAGTGAGGGAAAGAAGTTGGAGTCATTAAAACTCGTTT160.1576976148235758No Hit
ATGTCAGGAATTGTGAAAAATGAGGGAAAGAAGTTGGAGTCATTAAAACTCGTTT160.1576976148235758No Hit
ATGTCAGGAATTGTGAAAAGTGAGTTTACATACACTTAAGTTGGAGTCATTAAAACTCGTTT160.1576976148235758No Hit
ATGTCAGGAATTGTGAAAAAGTGAGGGAAAGAAGTTGGAGTATTAAAACTCGTTT140.13798541297062883No Hit
ATGTCAGAATTGTGAAAAAGTGAGGGAAAGAAGTTGGAGTCATTAAAACTCGTTT140.13798541297062883No Hit
ATGTCAGGAATTGTTAAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTA140.13798541297062883No Hit
ATGTCAGGAATTGTGAAAAAGTGAGTTTACATACACTTAAGTTGGAGTCATTAAACTCGTTT130.12812931204415534No Hit
ATGTCAGGAATTGTTAAAAAGTGAGGGAAAGAAGTTGGAGTCATTAAAACTCGTTT130.12812931204415534No Hit
ATGTCAGGAATTGTAAAAAGTGAGGGAAAGAAAGTTGGAGTCATTAAAACTCGTTT130.12812931204415534No Hit
ATGTCAGTAATTGTGAAAAAGTGAGGGAAAGAAAGTTGGAGTCATTAAAACTCGTTT120.11827321111768185No Hit
ATGTCAGAATTGTGAAAAAGTGAGTTTAAATGTATTTGGCTAAGGTGTAT120.11827321111768185No Hit
ATGTCAGGAATTGTAAAAAGTGAGGGAAAGAAGTTGGAGTCATTAAAACTCGTTT120.11827321111768185No Hit
ATGTAAGGAATTGTGAAAAAGTGAGGGAAAGAAAGTTGGAGTCATTAAAACTCGTTT120.11827321111768185No Hit
ATGTCAGGAATTGGAAAAAGTGAGGGAAAGAAAGTTGGAGTCATTAAAACTCGTTT120.11827321111768185No Hit
ATGTTAGGAATTGTGAAAAAGTGAGGGAAAGAAGTTGGAGTCATTAAAACTCGTTT120.11827321111768185No Hit
ATGTCAGGAATTGTGAAAAAGTGAGGGAAGGAAAGTTGGAGTCATTAAAACTCGTTT120.11827321111768185No Hit
ATGTCAGGAATTGTGAAAAAGTGAGTTTATATGTATTTGGCTAAGGTGTA120.11827321111768185No Hit
ATGTCAGGAATGTGAAAAAGTGAGAGAAAGAAGTTGGAGTCATTAAAACTCGTTT110.10841711019120837No Hit
ATGTCAGGATTGTGAAAAAGTGAGTTTACATACACTTAAGTTGGAGTCATTAAAACTCGTTT110.10841711019120837No Hit
ATGTCAGGAATTGTGAAAAAGTGAGGGAAAGAAAGTTGGGTCATTAAAACTCGTTT110.10841711019120837No Hit
ATGTCAGGAATTGTGAAAAAGTGAGGGAAGGAAGTTGGAGTCATTAAAACTCGTTT110.10841711019120837No Hit
ATGTCAGGAATTGTGAAAAAGTGAGGGAAAGAAGTTGGAGCATTAAAACTCGTTT110.10841711019120837No Hit
ATGTCAGGAATTGTGAAAAAGTGAGGGAAAGAAGTTGGAGTAATTAAAACTCGTTT110.10841711019120837No Hit
ATGTCAGGAATTGTGAAAAAGTGAGGGAAAGAAGTTGGATCATTAAAACTCGTTT110.10841711019120837No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)