[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 166508 sequences (15646778 bp)...
[M::mem_process_seqs] Processed 166508 reads in 21.209 CPU sec, 4.730 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada1 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub11_ada1.fq
[main] Real time: 62.748 sec; CPU: 24.186 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 166508 sequences (12648980 bp)...
[M::mem_process_seqs] Processed 166508 reads in 23.398 CPU sec, 4.806 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada2 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub11_ada2.fq
[main] Real time: 7.897 sec; CPU: 26.007 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 333016 sequences (28295758 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 80165, 48410, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (49, 70, 107)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 223)
[M::mem_pestat] mean and std.dev: (82.24, 45.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 281)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 3)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 3)
[M::mem_pestat] mean and std.dev: (3.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (3, 3)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 333016 reads in 62.307 CPU sec, 12.697 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub11_1.fq /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub11_2.fq
[main] Real time: 16.347 sec; CPU: 65.113 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 91642 sequences (8607280 bp)...
[M::mem_process_seqs] Processed 91642 reads in 11.538 CPU sec, 2.342 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada1 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub17_ada1.fq
[main] Real time: 5.044 sec; CPU: 14.059 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 91642 sequences (6960748 bp)...
[M::mem_process_seqs] Processed 91642 reads in 12.392 CPU sec, 2.496 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada2 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub17_ada2.fq
[main] Real time: 5.201 sec; CPU: 14.839 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 183284 sequences (15568028 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 51903, 20653, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (49, 57, 107)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 223)
[M::mem_pestat] mean and std.dev: (83.73, 51.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 281)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 3)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 3)
[M::mem_pestat] mean and std.dev: (3.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (3, 3)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 183284 reads in 31.122 CPU sec, 6.293 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub17_1.fq /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub17_2.fq
[main] Real time: 9.393 sec; CPU: 33.760 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 3788 sequences (355940 bp)...
[M::mem_process_seqs] Processed 3788 reads in 0.432 CPU sec, 0.089 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada1 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub26_ada1.fq
[main] Real time: 2.388 sec; CPU: 2.726 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 3788 sequences (287134 bp)...
[M::mem_process_seqs] Processed 3788 reads in 0.469 CPU sec, 0.096 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada2 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub26_ada2.fq
[main] Real time: 2.390 sec; CPU: 2.760 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 7576 sequences (643074 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 1192, 1570, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (70, 111, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (147.46, 97.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 3)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 3)
[M::mem_pestat] mean and std.dev: (3.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (3, 3)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 7576 reads in 1.433 CPU sec, 0.291 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub26_1.fq /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub26_2.fq
[main] Real time: 2.606 sec; CPU: 3.731 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 86782 sequences (8158232 bp)...
[M::mem_process_seqs] Processed 86782 reads in 10.395 CPU sec, 2.108 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada1 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub30_ada1.fq
[main] Real time: 4.652 sec; CPU: 12.770 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 86782 sequences (6588751 bp)...
[M::mem_process_seqs] Processed 86782 reads in 13.238 CPU sec, 2.669 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada2 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub30_ada2.fq
[main] Real time: 5.367 sec; CPU: 15.679 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 173564 sequences (14746983 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 43188, 23521, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (50, 106, 107)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 221)
[M::mem_pestat] mean and std.dev: (99.84, 53.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 278)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 3)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 3)
[M::mem_pestat] mean and std.dev: (3.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (3, 3)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 173564 reads in 35.411 CPU sec, 7.225 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub30_1.fq /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub30_2.fq
[main] Real time: 10.302 sec; CPU: 38.047 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 166368 sequences (15632079 bp)...
[M::mem_process_seqs] Processed 166368 reads in 22.385 CPU sec, 4.558 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada1 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub9_ada1.fq
[main] Real time: 7.492 sec; CPU: 24.968 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 166368 sequences (12635054 bp)...
[M::mem_process_seqs] Processed 166368 reads in 24.708 CPU sec, 4.998 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada2 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub9_ada2.fq
[main] Real time: 7.977 sec; CPU: 27.189 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 332736 sequences (28267133 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 73608, 54485, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (49, 91, 107)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 223)
[M::mem_pestat] mean and std.dev: (97.96, 55.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 281)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 3)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 3)
[M::mem_pestat] mean and std.dev: (3.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (3, 3)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 332736 reads in 61.695 CPU sec, 12.518 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub9_1.fq /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub9_2.fq
[main] Real time: 16.148 sec; CPU: 64.388 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 111343 sequences (10465558 bp)...
[M::mem_process_seqs] Processed 111343 reads in 12.639 CPU sec, 2.578 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada1 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub14_ada1.fq
[main] Real time: 5.273 sec; CPU: 15.034 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 111343 sequences (8440539 bp)...
[M::mem_process_seqs] Processed 111343 reads in 21.309 CPU sec, 4.337 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada2 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub14_ada2.fq
[main] Real time: 7.252 sec; CPU: 23.850 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 222686 sequences (18906097 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 27569, 38088, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (107, 186, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395)
[M::mem_pestat] mean and std.dev: (173.09, 58.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 491)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 3)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 3)
[M::mem_pestat] mean and std.dev: (3.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (3, 3)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 222686 reads in 53.163 CPU sec, 10.772 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub14_1.fq /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub14_2.fq
[main] Real time: 14.007 sec; CPU: 55.705 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 88018 sequences (8270327 bp)...
[M::mem_process_seqs] Processed 88018 reads in 12.661 CPU sec, 2.582 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada1 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub19_ada1.fq
[main] Real time: 5.098 sec; CPU: 14.994 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 88018 sequences (6684950 bp)...
[M::mem_process_seqs] Processed 88018 reads in 14.287 CPU sec, 2.871 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada2 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub19_ada2.fq
[main] Real time: 5.457 sec; CPU: 16.619 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 176036 sequences (14955277 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 40302, 25025, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (49, 70, 107)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 223)
[M::mem_pestat] mean and std.dev: (84.31, 49.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 281)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 3)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 3)
[M::mem_pestat] mean and std.dev: (3.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (3, 3)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 176036 reads in 37.356 CPU sec, 7.613 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub19_1.fq /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub19_2.fq
[main] Real time: 10.526 sec; CPU: 39.843 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 12590 sequences (1178725 bp)...
[M::mem_process_seqs] Processed 12590 reads in 1.294 CPU sec, 0.263 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada1 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub27_ada1.fq
[main] Real time: 2.455 sec; CPU: 3.467 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 12590 sequences (952980 bp)...
[M::mem_process_seqs] Processed 12590 reads in 1.737 CPU sec, 0.351 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada2 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub27_ada2.fq
[main] Real time: 2.568 sec; CPU: 3.926 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 25180 sequences (2131705 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 5762, 2569, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (58, 109, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577)
[M::mem_pestat] mean and std.dev: (148.34, 100.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 3)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 3)
[M::mem_pestat] mean and std.dev: (3.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (3, 3)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 25180 reads in 4.719 CPU sec, 0.949 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub27_1.fq /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub27_2.fq
[main] Real time: 3.229 sec; CPU: 6.940 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 95826 sequences (9003281 bp)...
[M::mem_process_seqs] Processed 95826 reads in 12.092 CPU sec, 2.466 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada1 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub4_ada1.fq
[main] Real time: 4.974 sec; CPU: 14.414 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 95826 sequences (7276030 bp)...
[M::mem_process_seqs] Processed 95826 reads in 12.773 CPU sec, 2.590 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada2 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub4_ada2.fq
[main] Real time: 5.113 sec; CPU: 15.018 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 191652 sequences (16279311 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44475, 27867, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (49, 70, 107)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 223)
[M::mem_pestat] mean and std.dev: (93.92, 54.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 281)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 3)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 3)
[M::mem_pestat] mean and std.dev: (3.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (3, 3)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 191652 reads in 31.786 CPU sec, 6.413 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub4_1.fq /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub4_2.fq
[main] Real time: 9.325 sec; CPU: 34.189 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 92998 sequences (8738152 bp)...
[M::mem_process_seqs] Processed 92998 reads in 16.277 CPU sec, 3.344 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada1 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub15_ada1.fq
[main] Real time: 5.892 sec; CPU: 18.610 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 92998 sequences (7064698 bp)...
[M::mem_process_seqs] Processed 92998 reads in 16.072 CPU sec, 3.310 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada2 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub15_ada2.fq
[main] Real time: 5.919 sec; CPU: 18.403 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 185996 sequences (15802850 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 39043, 28276, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (49, 51, 107)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 223)
[M::mem_pestat] mean and std.dev: (83.70, 50.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 281)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 3)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 3)
[M::mem_pestat] mean and std.dev: (3.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (3, 3)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 185996 reads in 41.840 CPU sec, 8.553 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub15_1.fq /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub15_2.fq
[main] Real time: 11.543 sec; CPU: 44.315 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 32570 sequences (3059824 bp)...
[M::mem_process_seqs] Processed 32570 reads in 3.748 CPU sec, 0.756 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada1 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub20_ada1.fq
[main] Real time: 3.148 sec; CPU: 6.076 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 32570 sequences (2467202 bp)...
[M::mem_process_seqs] Processed 32570 reads in 5.587 CPU sec, 1.124 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada2 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub20_ada2.fq
[main] Real time: 3.544 sec; CPU: 7.908 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 65140 sequences (5527026 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 10674, 8832, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (70, 107, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463)
[M::mem_pestat] mean and std.dev: (127.60, 66.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 594)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 3)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 3)
[M::mem_pestat] mean and std.dev: (3.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (3, 3)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 65140 reads in 14.054 CPU sec, 2.832 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub20_1.fq /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub20_2.fq
[main] Real time: 5.403 sec; CPU: 16.440 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 51699 sequences (4854020 bp)...
[M::mem_process_seqs] Processed 51699 reads in 7.892 CPU sec, 1.589 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada1 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub28_ada1.fq
[main] Real time: 4.059 sec; CPU: 10.251 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 51699 sequences (3921959 bp)...
[M::mem_process_seqs] Processed 51699 reads in 8.540 CPU sec, 1.728 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada2 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub28_ada2.fq
[main] Real time: 4.240 sec; CPU: 10.906 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 103398 sequences (8775979 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 22968, 14521, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (49, 70, 107)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 223)
[M::mem_pestat] mean and std.dev: (94.16, 54.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 281)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 3)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 3)
[M::mem_pestat] mean and std.dev: (3.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (3, 3)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 103398 reads in 21.204 CPU sec, 4.308 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub28_1.fq /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub28_2.fq
[main] Real time: 6.944 sec; CPU: 23.541 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 82795 sequences (7783714 bp)...
[M::mem_process_seqs] Processed 82795 reads in 8.102 CPU sec, 1.633 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada1 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub5_ada1.fq
[main] Real time: 4.068 sec; CPU: 10.344 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 82795 sequences (6281534 bp)...
[M::mem_process_seqs] Processed 82795 reads in 11.436 CPU sec, 2.301 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada2 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub5_ada2.fq
[main] Real time: 4.808 sec; CPU: 13.680 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 165590 sequences (14065248 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 27922, 25103, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (104, 107, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395)
[M::mem_pestat] mean and std.dev: (129.16, 56.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 492)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 3)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 3)
[M::mem_pestat] mean and std.dev: (3.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (3, 3)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 165590 reads in 29.994 CPU sec, 6.101 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub5_1.fq /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub5_2.fq
[main] Real time: 8.966 sec; CPU: 32.379 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 62093 sequences (5810682 bp)...
[M::mem_process_seqs] Processed 62093 reads in 6.916 CPU sec, 1.390 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada1 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub16_ada1.fq
[main] Real time: 3.749 sec; CPU: 9.153 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 62093 sequences (4694972 bp)...
[M::mem_process_seqs] Processed 62093 reads in 8.917 CPU sec, 1.801 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada2 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub16_ada2.fq
[main] Real time: 4.227 sec; CPU: 11.157 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 124186 sequences (10505654 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 31372, 14297, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (49, 107, 142)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328)
[M::mem_pestat] mean and std.dev: (108.37, 62.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 421)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 3)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 3)
[M::mem_pestat] mean and std.dev: (3.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (3, 3)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 124186 reads in 22.180 CPU sec, 4.538 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub16_1.fq /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub16_2.fq
[main] Real time: 7.200 sec; CPU: 24.496 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 64328 sequences (6039661 bp)...
[M::mem_process_seqs] Processed 64328 reads in 8.329 CPU sec, 1.676 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada1 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub21_ada1.fq
[main] Real time: 4.055 sec; CPU: 10.565 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 64328 sequences (4879977 bp)...
[M::mem_process_seqs] Processed 64328 reads in 9.483 CPU sec, 1.918 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada2 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub21_ada2.fq
[main] Real time: 4.327 sec; CPU: 11.704 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 128656 sequences (10919638 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 27887, 18935, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (49, 70, 107)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 223)
[M::mem_pestat] mean and std.dev: (86.36, 51.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 281)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 3)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 3)
[M::mem_pestat] mean and std.dev: (3.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (3, 3)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 128656 reads in 26.400 CPU sec, 5.406 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub21_1.fq /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub21_2.fq
[main] Real time: 8.134 sec; CPU: 28.777 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 66168 sequences (6212896 bp)...
[M::mem_process_seqs] Processed 66168 reads in 10.075 CPU sec, 2.041 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada1 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub29_ada1.fq
[main] Real time: 4.514 sec; CPU: 12.407 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 66168 sequences (5023215 bp)...
[M::mem_process_seqs] Processed 66168 reads in 11.686 CPU sec, 2.350 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada2 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub29_ada2.fq
[main] Real time: 4.868 sec; CPU: 14.012 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 132336 sequences (11236111 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 29147, 17482, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (49, 51, 107)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 223)
[M::mem_pestat] mean and std.dev: (87.39, 55.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 281)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 3)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 3)
[M::mem_pestat] mean and std.dev: (3.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (3, 3)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 132336 reads in 30.079 CPU sec, 6.139 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub29_1.fq /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub29_2.fq
[main] Real time: 8.865 sec; CPU: 32.451 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 109734 sequences (10305593 bp)...
[M::mem_process_seqs] Processed 109734 reads in 14.538 CPU sec, 2.934 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada1 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub7_ada1.fq
[main] Real time: 5.449 sec; CPU: 16.823 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 109734 sequences (8333322 bp)...
[M::mem_process_seqs] Processed 109734 reads in 14.673 CPU sec, 3.023 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M -R @RG\tID:${file}_ada2 /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub7_ada2.fq
[main] Real time: 5.613 sec; CPU: 16.938 sec
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 219468 sequences (18638915 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 51397, 31725, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (49, 70, 107)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 223)
[M::mem_pestat] mean and std.dev: (84.53, 49.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 281)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 3)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 3)
[M::mem_pestat] mean and std.dev: (3.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (3, 3)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 219468 reads in 38.652 CPU sec, 7.889 real sec
[main] Version: 0.7.13-r1126
[main] CMD: bwa mem -t 5 -M /mnt/storage/home/psbelokopytova/insulatory_index/bds_pipeline_genome_data/hg38/bwa_index/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub7_1.fq /mnt/storage/home/psbelokopytova/loxP_project/2022-06-14_rings//cutadapt/HAP8VIR_sub7_2.fq
[main] Real time: 10.882 sec; CPU: 41.098 sec