cutadapt -e 0.125 -g ATGTCAGA -j 16 --action=none -o untrimmed_SC5_R1_001.fastq.gz -p untrimmed_SC5_R2_001.fastq.gz Undetermined_S0_R1_001.fastq.gz Undetermined_S0_R2_001.fastq.gz This is cutadapt 2.6 with Python 3.7.16 Command line parameters: -e 0.125 -g ATGTCAGA -j 16 --action=none -o untrimmed_SC5_R1_001.fastq.gz -p untrimmed_SC5_R2_001.fastq.gz Undetermined_S0_R1_001.fastq.gz Undetermined_S0_R2_001.fastq.gz ^XProcessing reads on 16 cores in paired-end mode ... [-------=8 ] 02:31:50 533,660,953 reads @ 17.1 µs/read; 3.51 M reads/minute^[[3~ Finished in 9113.09 s (17 us/read; 3.51 M reads/minute). === Summary === Total read pairs processed: 533,660,953 Read 1 with adapter: 64,891,114 (12.2%) Read 2 with adapter: 0 (0.0%) Pairs written (passing filters): 533,660,953 (100.0%) Total basepairs processed: 159,570,593,183 bp Read 1: 79,900,650,894 bp Read 2: 79,669,942,289 bp Total written (filtered): 159,570,593,183 bp (100.0%) Read 1: 79,900,650,894 bp Read 2: 79,669,942,289 bp === First read: Adapter 1 === Sequence: ATGTCAGA; Type: regular 5'; Length: 8; Trimmed: 64891114 times. No. of allowed errors: 0-7 bp: 0; 8 bp: 1 Overview of removed sequences length count expect max.err error counts 3 7787382 8338452.4 0 7787382 4 2365563 2084613.1 0 2365563 5 428509 521153.3 0 428509 6 180729 130288.3 0 180729 7 204569 32572.1 0 8438 196131 8 318515 8143.0 1 1156 317359 9 323663 8143.0 1 1066 322597 10 422657 8143.0 1 1179 421478 11 389869 8143.0 1 2537 387332 12 480792 8143.0 1 2351 478441 13 409834 8143.0 1 4655 405179 14 371629 8143.0 1 4999 366630 15 397061 8143.0 1 2543 394518 16 443621 8143.0 1 2499 441122 17 401528 8143.0 1 2779 398749 18 386137 8143.0 1 2596 383541 19 381147 8143.0 1 2834 378313 20 386085 8143.0 1 3129 382956 21 419792 8143.0 1 3075 416717 22 364681 8143.0 1 2484 362197 23 405132 8143.0 1 2940 402192 24 401792 8143.0 1 2702 399090 25 364659 8143.0 1 2625 362034 26 358739 8143.0 1 3568 355171 27 394821 8143.0 1 2499 392322 28 380715 8143.0 1 2773 377942 29 397434 8143.0 1 2388 395046 30 425555 8143.0 1 2642 422913 31 380316 8143.0 1 2484 377832 32 425869 8143.0 1 2753 423116 33 417266 8143.0 1 2609 414657 34 399360 8143.0 1 2905 396455 35 349856 8143.0 1 2515 347341 36 399871 8143.0 1 2879 396992 37 404308 8143.0 1 3551 400757 38 432976 8143.0 1 2468 430508 39 412995 8143.0 1 2573 410422 40 350862 8143.0 1 2520 348342 41 431765 8143.0 1 2746 429019 42 457038 8143.0 1 2851 454187 43 341944 8143.0 1 2567 339377 44 377286 8143.0 1 3270 374016 45 368326 8143.0 1 3563 364763 46 338652 8143.0 1 2632 336020 47 359013 8143.0 1 2634 356379 48 396769 8143.0 1 2746 394023 49 409456 8143.0 1 2684 406772 50 383682 8143.0 1 2688 380994 51 373348 8143.0 1 2408 370940 52 357471 8143.0 1 2737 354734 53 386413 8143.0 1 2642 383771 54 410939 8143.0 1 2472 408467 55 343936 8143.0 1 2554 341382 56 343997 8143.0 1 2418 341579 57 386640 8143.0 1 2527 384113 58 358025 8143.0 1 2438 355587 59 362050 8143.0 1 2989 359061 60 393303 8143.0 1 2704 390599 61 401401 8143.0 1 2590 398811 62 396155 8143.0 1 2836 393319 63 392431 8143.0 1 2760 389671 64 400145 8143.0 1 2670 397475 65 378847 8143.0 1 2885 375962 66 398190 8143.0 1 2771 395419 67 417909 8143.0 1 2552 415357 68 368655 8143.0 1 2640 366015 69 404056 8143.0 1 2605 401451 70 388827 8143.0 1 2685 386142 71 387532 8143.0 1 2614 384918 72 396078 8143.0 1 2572 393506 73 350144 8143.0 1 2501 347643 74 357799 8143.0 1 2620 355179 75 386794 8143.0 1 2722 384072 76 362224 8143.0 1 2618 359606 77 351590 8143.0 1 2760 348830 78 377758 8143.0 1 2690 375068 79 368382 8143.0 1 2837 365545 80 379478 8143.0 1 3057 376421 81 364764 8143.0 1 2766 361998 82 357841 8143.0 1 2835 355006 83 379978 8143.0 1 2631 377347 84 378404 8143.0 1 2584 375820 85 366769 8143.0 1 2819 363950 86 361494 8143.0 1 2674 358820 87 378958 8143.0 1 2631 376327 88 366487 8143.0 1 2852 363635 89 358927 8143.0 1 2581 356346 90 356774 8143.0 1 2848 353926 91 349678 8143.0 1 2809 346869 92 354780 8143.0 1 3371 351409 93 372412 8143.0 1 2674 369738 94 380358 8143.0 1 2653 377705 95 343601 8143.0 1 2614 340987 96 358008 8143.0 1 3072 354936 97 358816 8143.0 1 2808 356008 98 417904 8143.0 1 2623 415281 99 371252 8143.0 1 2563 368689 100 342262 8143.0 1 2647 339615 101 378109 8143.0 1 2589 375520 102 393836 8143.0 1 2723 391113 103 345279 8143.0 1 2578 342701 104 382138 8143.0 1 3578 378560 105 350994 8143.0 1 2668 348326 106 353941 8143.0 1 2774 351167 107 381012 8143.0 1 2779 378233 108 339870 8143.0 1 2724 337146 109 386740 8143.0 1 2718 384022 110 328080 8143.0 1 2478 325602 111 322492 8143.0 1 2960 319532 112 333791 8143.0 1 2726 331065 113 337746 8143.0 1 2872 334874 114 412353 8143.0 1 3115 409238 115 365433 8143.0 1 2880 362553 116 347272 8143.0 1 2925 344347 117 318929 8143.0 1 3067 315862 118 336013 8143.0 1 2967 333046 119 374235 8143.0 1 3131 371104 120 392369 8143.0 1 2956 389413 121 361945 8143.0 1 2965 358980 122 380743 8143.0 1 2961 377782 123 413275 8143.0 1 2739 410536 124 385039 8143.0 1 2933 382106 125 344294 8143.0 1 2984 341310 126 374624 8143.0 1 2808 371816 127 376938 8143.0 1 2890 374048 128 377237 8143.0 1 2883 374354 129 389275 8143.0 1 3153 386122 130 355564 8143.0 1 3189 352375 131 341676 8143.0 1 2753 338923 132 368197 8143.0 1 2753 365444 133 342980 8143.0 1 3223 339757 134 361899 8143.0 1 2893 359006 135 411099 8143.0 1 2822 408277 136 404697 8143.0 1 2663 402034 137 415083 8143.0 1 2948 412135 138 377576 8143.0 1 2880 374696 139 352129 8143.0 1 2830 349299 140 427799 8143.0 1 2752 425047 141 416443 8143.0 1 2780 413663 142 439667 8143.0 1 2874 436793 143 384794 8143.0 1 2696 382098 144 385129 8143.0 1 2866 382263 145 375232 8143.0 1 2770 372462 146 376004 8143.0 1 2798 373206 147 364766 8143.0 1 2910 361856 148 330350 8143.0 1 2844 327506 149 319722 8143.0 1 2585 317137 150 226102 8143.0 1 1234 224868