This is cutadapt 5.1 with Python 3.12.5 Command line parameters: -a AGATCGGAAGAGCACACGTCTGAACTCCAGTCA -A AGCTGAATGTAATTGCGCATACCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT -A AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT -m 70 -o FilteredReads/R1.trim.fq.gz -p FilteredReads/R2.trim.fq.gz RawReads/LT2G2D_L01_R1.fq.gz RawReads/LT2G2D_L01_R2.fq.gz --report=full Processing paired-end reads on 1 core ... === Summary === Total read pairs processed: 1,986 Read 1 with adapter: 226 (11.4%) Read 2 with adapter: 362 (18.2%) == Read fate breakdown == Pairs that were too short: 153 (7.7%) Pairs written (passing filters): 1,833 (92.3%) Total basepairs processed: 595,800 bp Read 1: 297,900 bp Read 2: 297,900 bp Total written (filtered): 545,240 bp (91.5%) Read 1: 273,566 bp Read 2: 271,674 bp === First read: Adapter 1 === Sequence: AGATCGGAAGAGCACACGTCTGAACTCCAGTCA; Type: regular 3'; Length: 33; Trimmed: 226 times Minimum overlap: 3 No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-33 bp: 3 Bases preceding removed adapters: A: 6.6% C: 65.5% G: 15.0% T: 12.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 19 31.0 0 19 4 17 7.8 0 17 12 6 0.0 1 4 2 14 1 0.0 1 1 16 3 0.0 1 2 1 20 1 0.0 2 1 21 4 0.0 2 4 22 1 0.0 2 1 27 2 0.0 2 2 29 4 0.0 2 3 1 38 4 0.0 3 4 41 2 0.0 3 1 1 43 1 0.0 3 1 50 6 0.0 3 5 1 51 2 0.0 3 2 56 4 0.0 3 4 57 6 0.0 3 3 3 59 8 0.0 3 8 60 1 0.0 3 1 61 3 0.0 3 1 2 68 7 0.0 3 7 69 4 0.0 3 4 70 10 0.0 3 9 1 71 15 0.0 3 14 1 72 1 0.0 3 1 73 3 0.0 3 2 0 1 74 1 0.0 3 0 0 1 75 2 0.0 3 2 76 8 0.0 3 8 78 2 0.0 3 2 79 1 0.0 3 1 80 1 0.0 3 1 90 1 0.0 3 0 0 0 1 95 2 0.0 3 1 1 98 1 0.0 3 1 126 23 0.0 3 14 8 1 127 25 0.0 3 2 14 8 1 128 4 0.0 3 1 3 129 19 0.0 3 9 9 1 130 1 0.0 3 1 === Second read: Adapter 2 === Sequence: AGCTGAATGTAATTGCGCATACCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT; Type: regular 3'; Length: 58; Trimmed: 299 times Minimum overlap: 3 No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40-49 bp: 4; 50-58 bp: 5 Bases preceding removed adapters: A: 16.1% C: 41.8% G: 26.8% T: 3.3% none/other: 12.0% Overview of removed sequences length count expect max.err error counts 3 68 31.0 0 68 4 34 7.8 0 34 5 10 1.9 0 10 12 8 0.0 1 8 14 13 0.0 1 13 16 6 0.0 1 0 6 20 2 0.0 2 2 27 4 0.0 2 4 36 1 0.0 3 0 1 37 6 0.0 3 0 5 1 39 1 0.0 3 1 41 3 0.0 4 3 45 1 0.0 4 1 46 4 0.0 4 1 2 1 47 1 0.0 4 1 52 2 0.0 5 0 2 54 4 0.0 5 3 1 63 4 0.0 5 4 65 2 0.0 5 0 2 68 1 0.0 5 0 1 75 8 0.0 5 5 3 79 1 0.0 5 0 1 81 4 0.0 5 0 4 82 6 0.0 5 3 3 84 9 0.0 5 1 5 2 1 85 3 0.0 5 0 1 2 92 2 0.0 5 0 0 2 93 8 0.0 5 4 4 94 1 0.0 5 1 95 10 0.0 5 7 2 1 96 15 0.0 5 0 11 4 97 1 0.0 5 0 1 98 4 0.0 5 0 2 2 100 2 0.0 5 0 1 1 101 7 0.0 5 0 6 1 103 2 0.0 5 2 104 2 0.0 5 0 2 120 2 0.0 5 2 123 1 0.0 5 0 0 1 150 36 0.0 5 0 12 4 2 11 7 === Second read: Adapter 3 === Sequence: AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT; Type: regular 3'; Length: 33; Trimmed: 63 times Minimum overlap: 3 No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-33 bp: 3 Bases preceding removed adapters: A: 12.7% C: 1.6% G: 81.0% T: 4.8% none/other: 0.0% WARNING: The adapter is preceded by 'G' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 56 31.0 0 56 4 1 7.8 0 1 16 1 0.0 1 1 28 1 0.0 2 1 33 1 0.0 3 0 1 102 1 0.0 3 1 129 1 0.0 3 0 0 1 130 1 0.0 3 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.