Bismark report for: MoPh14_1.fq.gz and MoPh14_2.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /media/eternus1/nfs/projects/dpanchenko/komarek/ChiPseq/bismark_genome/ with the specified options: -q --score-min L,0,-0.2 -p 40 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 360314625 Number of paired-end alignments with a unique best hit: 180087696 Mapping efficiency: 50.0% Sequence pairs with no alignments under any condition: 168200818 Sequence pairs did not map uniquely: 12026111 Sequence pairs which were discarded because genomic sequence could not be extracted: 1 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 90063369 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 90024326 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 10973758332 Total methylated C's in CpG context: 474987968 Total methylated C's in CHG context: 14828190 Total methylated C's in CHH context: 39844155 Total methylated C's in Unknown context: 60598 Total unmethylated C's in CpG context: 239743547 Total unmethylated C's in CHG context: 2547926528 Total unmethylated C's in CHH context: 7656427944 Total unmethylated C's in Unknown context: 4004980 C methylated in CpG context: 66.5% C methylated in CHG context: 0.6% C methylated in CHH context: 0.5% C methylated in Unknown context (CN or CHN): 1.5% Bismark completed in 0d 20h 26m 10s