This is cutadapt 2.8 with Python 3.8.10 Command line parameters: -j 10 -e 0.2 -m 8 -a AGATCGGAAGAGCACACGTCTGAACTCCAGTCA -A AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT -o /tmp/tmppmeplvrj/adapter_R1_0.fastq.gz -p /tmp/tmppmeplvrj/adapter_R2_0.fastq.gz /Data/NGS_Data/2022-07-25-BGI/Demult/P100_R1.fastq.gz /Data/NGS_Data/2022-07-25-BGI/Demult/P100_R2.fastq.gz Processing reads on 10 cores in paired-end mode ... Finished in 578.60 s (18 us/read; 3.30 M reads/minute). === Summary === Total read pairs processed: 31,815,375 Read 1 with adapter: 2,409,734 (7.6%) Read 2 with adapter: 55,457 (0.2%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 31,815,375 (100.0%) Total basepairs processed: 10,180,920,000 bp Read 1: 4,772,306,250 bp Read 2: 5,408,613,750 bp Total written (filtered): 10,166,001,287 bp (99.9%) Read 1: 4,759,234,029 bp Read 2: 5,406,767,258 bp === First read: Adapter 1 === Sequence: AGATCGGAAGAGCACACGTCTGAACTCCAGTCA; Type: regular 3'; Length: 33; Trimmed: 2409734 times; Reverse-complemented: 0 times No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20-24 bp: 4; 25-29 bp: 5; 30-33 bp: 6 Bases preceding removed adapters: A: 28.5% C: 28.3% G: 26.2% T: 17.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 765507 497115.2 0 765507 4 479277 124278.8 0 202235 277042 5 575749 31069.7 1 86523 489226 6 189731 7767.4 1 55835 133896 7 79835 1941.9 1 47540 32295 8 55900 485.5 1 33846 11171 10883 9 55053 121.4 1 31101 3877 20075 10 44958 30.3 2 23982 1776 19200 11 31865 7.6 2 19891 1120 10854 12 21769 1.9 2 16502 668 4237 362 13 16762 0.5 2 13971 584 1262 945 14 13359 0.1 2 11108 486 386 1379 15 10591 0.0 3 9002 418 185 986 16 8854 0.0 3 7858 355 81 548 12 17 7332 0.0 3 6613 359 64 264 32 18 5890 0.0 3 5340 340 67 83 60 19 4899 0.0 3 4491 255 55 39 59 20 4144 0.0 4 3745 235 56 28 51 29 21 3447 0.0 4 3135 197 47 20 18 30 22 3476 0.0 4 3123 274 42 16 15 6 23 3231 0.0 4 2893 230 47 39 17 5 24 3076 0.0 4 2743 224 51 24 10 21 3 25 2726 0.0 5 2396 245 53 11 9 12 26 2613 0.0 5 2319 211 46 15 3 8 11 27 2428 0.0 5 2138 198 46 8 19 14 5 28 2096 0.0 5 1823 187 32 17 32 5 29 1730 0.0 5 1506 143 36 19 20 6 30 1466 0.0 6 1280 135 39 10 2 31 1242 0.0 6 1054 150 25 7 6 32 1111 0.0 6 952 111 33 6 7 0 2 33 1075 0.0 6 921 110 25 13 2 2 2 34 469 0.0 6 391 55 15 4 3 0 1 35 849 0.0 6 742 84 15 5 2 0 1 36 781 0.0 6 664 74 27 6 4 5 1 37 674 0.0 6 569 90 10 4 0 1 38 647 0.0 6 557 64 16 3 3 3 1 39 527 0.0 6 434 63 21 5 3 0 1 40 390 0.0 6 329 43 11 4 2 1 41 395 0.0 6 331 47 10 6 1 42 302 0.0 6 254 36 8 1 3 43 15 0.0 6 10 2 1 0 1 1 44 157 0.0 6 119 28 5 5 45 207 0.0 6 163 36 7 0 1 46 196 0.0 6 156 23 14 1 1 1 47 230 0.0 6 196 29 5 48 210 0.0 6 173 34 2 1 49 159 0.0 6 138 15 3 3 50 152 0.0 6 123 24 3 1 0 0 1 51 148 0.0 6 128 13 5 2 52 115 0.0 6 97 10 4 2 1 0 1 53 79 0.0 6 67 10 2 54 98 0.0 6 86 11 0 0 0 1 55 97 0.0 6 84 11 2 56 82 0.0 6 68 11 1 1 0 1 57 71 0.0 6 58 8 2 0 0 3 58 60 0.0 6 49 7 2 2 59 88 0.0 6 57 17 7 3 3 1 60 39 0.0 6 32 7 61 31 0.0 6 27 3 0 1 62 46 0.0 6 29 13 3 0 1 63 22 0.0 6 16 6 64 33 0.0 6 24 7 2 65 22 0.0 6 15 5 2 66 10 0.0 6 8 1 0 1 67 10 0.0 6 6 4 68 10 0.0 6 4 4 1 0 0 0 1 69 9 0.0 6 9 70 8 0.0 6 7 0 1 71 47 0.0 6 35 10 0 0 2 72 14 0.0 6 11 2 1 73 26 0.0 6 14 6 3 3 74 54 0.0 6 39 10 4 1 75 21 0.0 6 12 4 2 3 76 43 0.0 6 31 10 1 0 1 77 56 0.0 6 39 9 6 2 78 14 0.0 6 10 2 2 79 41 0.0 6 25 15 1 80 37 0.0 6 19 10 6 2 81 14 0.0 6 9 5 82 23 0.0 6 22 1 83 7 0.0 6 6 1 84 4 0.0 6 3 1 86 10 0.0 6 7 2 1 87 278 0.0 6 173 69 23 7 3 1 2 88 159 0.0 6 102 38 14 2 2 1 89 26 0.0 6 18 6 2 90 8 0.0 6 6 2 91 34 0.0 6 25 9 92 10 0.0 6 10 93 4 0.0 6 2 1 1 96 2 0.0 6 2 97 98 0.0 6 78 16 4 98 50 0.0 6 40 9 1 99 13 0.0 6 11 2 100 4 0.0 6 2 2 101 2 0.0 6 1 0 0 1 112 1 0.0 6 1 119 1 0.0 6 1 120 1 0.0 6 1 124 1 0.0 6 0 1 128 1 0.0 6 1 === Second read: Adapter 2 === Sequence: AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT; Type: regular 3'; Length: 33; Trimmed: 55457 times; Reverse-complemented: 0 times No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20-24 bp: 4; 25-29 bp: 5; 30-33 bp: 6 Bases preceding removed adapters: A: 15.3% C: 14.1% G: 50.0% T: 20.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 20463 497115.2 0 20463 4 190 124278.8 0 0 190 5 255 31069.7 1 0 255 40 6 0.0 6 0 0 0 0 0 0 6 42 3211 0.0 6 0 0 0 0 0 1 3210 43 3169 0.0 6 0 0 0 0 0 3039 130 44 3047 0.0 6 0 0 0 0 2868 131 48 45 2715 0.0 6 0 0 0 2222 369 100 24 46 2622 0.0 6 0 0 1945 540 102 26 9 47 2422 0.0 6 0 0 0 1797 522 82 21 48 2105 0.0 6 0 0 1453 93 476 68 15 49 1728 0.0 6 0 0 0 1289 68 290 81 50 1468 0.0 6 0 0 1006 80 44 14 324 51 1245 0.0 6 0 0 1097 91 36 12 9 52 1110 0.0 6 0 11 1 981 74 32 11 53 1074 0.0 6 942 64 17 5 29 11 6 54 472 0.0 6 396 42 7 7 2 10 8 55 856 0.0 6 749 71 10 7 4 0 15 56 782 0.0 6 691 63 16 9 1 2 57 677 0.0 6 599 46 6 5 14 2 5 58 649 0.0 6 573 42 9 3 8 13 1 59 529 0.0 6 467 40 5 4 8 4 1 60 390 0.0 6 326 38 9 1 9 4 3 61 395 0.0 6 355 21 6 2 7 3 1 62 301 0.0 6 260 26 2 3 5 3 2 63 16 0.0 6 5 6 0 0 2 2 1 64 158 0.0 6 122 25 1 2 3 4 1 65 207 0.0 6 170 23 3 1 6 2 2 66 195 0.0 6 161 22 2 0 3 5 2 67 230 0.0 6 198 24 2 1 3 2 68 211 0.0 6 178 17 4 2 7 3 69 159 0.0 6 142 10 4 2 0 1 70 151 0.0 6 126 16 6 0 3 71 155 0.0 6 124 19 2 1 2 7 72 113 0.0 6 101 5 3 2 1 0 1 73 80 0.0 6 69 5 2 1 1 2 74 98 0.0 6 87 7 2 0 1 0 1 75 98 0.0 6 86 9 2 1 76 81 0.0 6 72 6 1 1 1 77 73 0.0 6 67 5 0 0 1 78 61 0.0 6 57 3 0 0 1 79 89 0.0 6 78 10 1 80 37 0.0 6 31 3 2 0 1 81 30 0.0 6 27 1 2 82 47 0.0 6 35 7 3 0 1 0 1 83 24 0.0 6 17 6 0 1 84 38 0.0 6 30 7 0 1 85 25 0.0 6 16 6 3 86 12 0.0 6 7 3 1 0 0 0 1 87 9 0.0 6 8 1 88 11 0.0 6 10 1 89 9 0.0 6 8 1 90 7 0.0 6 5 1 1 91 51 0.0 6 36 10 2 2 1 92 18 0.0 6 14 3 1 93 30 0.0 6 24 3 1 0 1 0 1 94 59 0.0 6 38 14 3 1 1 0 2 95 23 0.0 6 16 6 1 96 44 0.0 6 27 9 7 0 1 97 58 0.0 6 38 14 4 1 1 98 16 0.0 6 10 3 2 0 0 0 1 99 42 0.0 6 31 9 1 1 100 38 0.0 6 28 9 0 1 101 15 0.0 6 10 5 102 24 0.0 6 16 6 2 103 7 0.0 6 5 2 104 4 0.0 6 4 106 10 0.0 6 6 3 0 0 1 107 283 0.0 6 203 64 9 3 2 0 2 108 164 0.0 6 124 32 3 1 3 0 1 109 28 0.0 6 21 6 1 110 10 0.0 6 6 1 0 1 0 0 2 111 35 0.0 6 25 8 1 0 0 0 1 112 10 0.0 6 8 2 113 4 0.0 6 4 115 1 0.0 6 0 0 0 0 0 0 1 116 2 0.0 6 2 117 99 0.0 6 79 18 2 118 52 0.0 6 37 11 4 119 13 0.0 6 9 3 1 120 4 0.0 6 3 0 1 121 2 0.0 6 0 2 132 1 0.0 6 1 139 1 0.0 6 1 140 1 0.0 6 1 144 1 0.0 6 1 148 2 0.0 6 1 0 0 0 0 0 1