This is cutadapt 2.8 with Python 3.8.10 Command line parameters: -j 10 -e 0.2 -m 8 -a AGATCGGAAGAGCACACGTCTGAACTCCAGTCA -A AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT -o /tmp/tmpypv6qdi1/adapter_R1_0.fastq.gz -p /tmp/tmpypv6qdi1/adapter_R2_0.fastq.gz /Data/NGS_Data/2022-07-25-BGI/Demult/P101_R1.fastq.gz /Data/NGS_Data/2022-07-25-BGI/Demult/P101_R2.fastq.gz Processing reads on 10 cores in paired-end mode ... Finished in 557.18 s (18 us/read; 3.32 M reads/minute). === Summary === Total read pairs processed: 30,820,190 Read 1 with adapter: 2,113,968 (6.9%) Read 2 with adapter: 28,607 (0.1%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 30,820,190 (100.0%) Total basepairs processed: 9,862,460,800 bp Read 1: 4,623,028,500 bp Read 2: 5,239,432,300 bp Total written (filtered): 9,850,689,620 bp (99.9%) Read 1: 4,612,369,210 bp Read 2: 5,238,320,410 bp === First read: Adapter 1 === Sequence: AGATCGGAAGAGCACACGTCTGAACTCCAGTCA; Type: regular 3'; Length: 33; Trimmed: 2113968 times; Reverse-complemented: 0 times No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20-24 bp: 4; 25-29 bp: 5; 30-33 bp: 6 Bases preceding removed adapters: A: 28.5% C: 28.7% G: 26.7% T: 16.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 717301 481565.5 0 717301 4 440908 120391.4 0 167350 273558 5 534703 30097.8 1 55483 479220 6 157939 7524.5 1 27796 130143 7 53908 1881.1 1 23039 30869 8 38302 470.3 1 16894 10826 10582 9 38151 117.6 1 15057 3548 19546 10 31677 29.4 2 11509 1562 18606 11 20769 7.3 2 9549 850 10370 12 12990 1.8 2 8109 530 4036 315 13 9637 0.5 2 7092 474 1176 895 14 8132 0.1 2 6021 386 432 1293 15 6788 0.0 3 5260 360 171 997 16 5777 0.0 3 4784 386 64 534 9 17 4509 0.0 3 3826 317 50 275 41 18 3766 0.0 3 3304 289 37 73 63 19 3034 0.0 3 2661 245 39 34 55 20 2550 0.0 4 2256 197 41 11 27 18 21 2089 0.0 4 1825 168 37 9 30 20 22 2031 0.0 4 1797 189 27 5 12 1 23 1836 0.0 4 1582 184 35 22 8 5 24 1752 0.0 4 1496 197 27 14 7 11 25 1665 0.0 5 1436 169 36 10 9 5 26 1502 0.0 5 1312 149 24 6 6 1 4 27 1344 0.0 5 1143 166 19 2 7 6 1 28 2302 0.0 5 1760 402 105 16 13 5 1 29 1210 0.0 5 964 176 39 8 20 3 30 806 0.0 6 682 91 16 10 6 1 31 696 0.0 6 568 95 26 5 2 32 594 0.0 6 494 80 12 6 1 0 1 33 525 0.0 6 425 79 16 3 1 1 34 250 0.0 6 200 34 12 1 0 2 1 35 458 0.0 6 368 64 21 4 1 36 394 0.0 6 310 55 20 4 5 37 357 0.0 6 294 51 9 2 1 38 334 0.0 6 270 50 9 3 0 2 39 435 0.0 6 318 80 26 4 3 3 1 40 221 0.0 6 163 44 9 1 2 1 1 41 198 0.0 6 156 31 5 5 0 0 1 42 145 0.0 6 105 28 11 1 43 4 0.0 6 2 2 44 69 0.0 6 51 14 3 1 45 99 0.0 6 83 10 4 0 1 1 46 88 0.0 6 73 12 2 1 47 85 0.0 6 67 15 2 0 0 1 48 99 0.0 6 82 12 3 1 0 1 49 77 0.0 6 56 13 8 50 101 0.0 6 74 18 5 4 51 77 0.0 6 58 15 3 1 52 52 0.0 6 40 10 1 1 53 29 0.0 6 17 9 2 0 1 54 35 0.0 6 20 6 6 1 1 0 1 55 43 0.0 6 35 8 56 30 0.0 6 24 2 4 57 30 0.0 6 23 5 2 58 17 0.0 6 13 3 0 1 59 47 0.0 6 27 13 6 0 1 60 26 0.0 6 20 3 3 61 9 0.0 6 7 1 1 62 32 0.0 6 17 8 4 1 1 1 63 18 0.0 6 13 3 2 64 20 0.0 6 11 4 3 1 1 65 12 0.0 6 11 0 1 66 8 0.0 6 6 1 0 0 0 1 67 4 0.0 6 4 68 4 0.0 6 3 1 69 4 0.0 6 4 70 3 0.0 6 3 71 17 0.0 6 10 2 2 2 1 72 11 0.0 6 8 2 1 73 25 0.0 6 17 6 2 74 31 0.0 6 17 12 2 75 11 0.0 6 8 1 1 1 76 33 0.0 6 25 8 77 48 0.0 6 34 9 3 2 78 9 0.0 6 8 1 79 26 0.0 6 16 7 2 0 1 80 21 0.0 6 12 5 4 81 5 0.0 6 3 0 1 1 82 14 0.0 6 11 1 1 1 83 5 0.0 6 3 2 84 5 0.0 6 4 0 1 86 11 0.0 6 5 4 2 87 254 0.0 6 153 68 25 5 1 1 1 88 136 0.0 6 101 24 8 2 0 0 1 89 27 0.0 6 16 4 5 2 90 5 0.0 6 4 0 1 91 29 0.0 6 20 6 2 1 92 10 0.0 6 5 4 1 93 3 0.0 6 2 0 1 96 1 0.0 6 0 1 97 58 0.0 6 44 12 0 2 98 41 0.0 6 32 6 3 99 10 0.0 6 8 1 1 100 2 0.0 6 2 102 1 0.0 6 1 107 1 0.0 6 0 0 1 109 1 0.0 6 1 113 1 0.0 6 1 116 1 0.0 6 1 124 7 0.0 6 6 1 130 1 0.0 6 1 === Second read: Adapter 2 === Sequence: AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT; Type: regular 3'; Length: 33; Trimmed: 28607 times; Reverse-complemented: 0 times No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20-24 bp: 4; 25-29 bp: 5; 30-33 bp: 6 Bases preceding removed adapters: A: 15.9% C: 17.4% G: 36.7% T: 30.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 5774 481565.5 0 5774 4 1056 120391.4 0 17 1039 5 786 30097.8 1 0 786 12 6 1.8 2 0 0 6 40 5 0.0 6 0 0 0 0 0 0 5 42 1919 0.0 6 0 0 0 0 0 0 1919 43 1804 0.0 6 0 0 0 0 0 1680 124 44 1735 0.0 6 0 0 0 0 1583 82 70 45 1664 0.0 6 0 0 0 1335 250 54 25 46 1502 0.0 6 0 0 1 277 46 53 1125 47 1346 0.0 6 0 0 0 997 268 42 39 48 2321 0.0 6 0 0 1544 91 582 79 25 49 1211 0.0 6 0 0 0 876 58 236 41 50 804 0.0 6 0 0 568 38 22 11 165 51 694 0.0 6 0 0 619 47 15 8 5 52 596 0.0 6 0 6 1 527 44 11 7 53 525 0.0 6 467 33 6 4 11 3 1 54 251 0.0 6 213 25 7 3 1 1 1 55 460 0.0 6 412 32 10 3 2 1 56 395 0.0 6 357 27 7 2 0 1 1 57 360 0.0 6 316 34 3 4 3 58 334 0.0 6 310 17 2 1 2 2 59 441 0.0 6 373 38 6 3 6 11 4 60 224 0.0 6 187 21 7 1 2 3 3 61 199 0.0 6 179 14 1 2 1 1 1 62 145 0.0 6 126 15 3 0 1 63 4 0.0 6 1 2 1 64 70 0.0 6 53 12 2 1 0 1 1 65 100 0.0 6 87 10 2 0 0 0 1 66 88 0.0 6 70 12 0 1 5 67 86 0.0 6 81 3 0 1 1 68 101 0.0 6 88 9 1 1 0 1 1 69 77 0.0 6 69 5 3 70 106 0.0 6 88 14 1 1 1 0 1 71 82 0.0 6 71 4 5 0 1 0 1 72 52 0.0 6 47 3 1 0 1 73 30 0.0 6 23 4 0 1 0 0 2 74 36 0.0 6 30 3 0 0 0 0 3 75 43 0.0 6 38 3 0 1 1 76 30 0.0 6 25 1 1 0 1 2 77 31 0.0 6 28 2 1 78 17 0.0 6 15 2 79 46 0.0 6 39 6 1 80 25 0.0 6 16 5 0 1 2 0 1 81 9 0.0 6 7 2 82 35 0.0 6 24 9 1 0 0 0 1 83 19 0.0 6 12 7 84 21 0.0 6 16 4 1 85 13 0.0 6 9 2 0 0 0 1 1 86 13 0.0 6 10 1 2 87 4 0.0 6 3 1 88 6 0.0 6 3 1 2 89 4 0.0 6 4 90 3 0.0 6 3 91 16 0.0 6 11 4 1 92 11 0.0 6 8 3 93 27 0.0 6 16 7 3 0 1 94 38 0.0 6 20 12 3 3 95 13 0.0 6 10 2 0 1 96 31 0.0 6 20 8 2 1 97 49 0.0 6 32 11 3 2 1 98 10 0.0 6 8 2 99 28 0.0 6 15 7 3 0 1 1 1 100 24 0.0 6 14 6 2 0 2 101 5 0.0 6 5 102 14 0.0 6 9 5 103 5 0.0 6 5 104 5 0.0 6 3 2 106 11 0.0 6 7 2 1 0 1 107 258 0.0 6 188 58 7 0 3 2 108 141 0.0 6 103 29 2 1 3 2 1 109 29 0.0 6 20 8 0 1 110 5 0.0 6 4 0 1 111 32 0.0 6 23 6 1 0 1 1 112 10 0.0 6 8 2 113 4 0.0 6 2 0 2 115 1 0.0 6 0 1 116 1 0.0 6 1 117 60 0.0 6 42 14 3 1 118 42 0.0 6 32 7 2 1 119 11 0.0 6 6 3 1 0 1 120 2 0.0 6 1 1 122 1 0.0 6 0 0 0 0 0 0 1 123 1 0.0 6 0 0 0 0 0 0 1 127 1 0.0 6 1 129 1 0.0 6 1 133 1 0.0 6 1 136 1 0.0 6 1 142 1 0.0 6 0 0 0 0 0 0 1 144 7 0.0 6 5 2 150 2 0.0 6 1 0 0 0 0 0 1