This is cutadapt 2.8 with Python 3.8.10 Command line parameters: -j 10 -e 0.2 -m 8 -a AGATCGGAAGAGCACACGTCTGAACTCCAGTCA -A AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT -o /tmp/tmpr1a5zzp0/adapter_R1_0.fastq.gz -p /tmp/tmpr1a5zzp0/adapter_R2_0.fastq.gz /Data/NGS_Data/2022-07-25-BGI/Demult/P96_R1.fastq.gz /Data/NGS_Data/2022-07-25-BGI/Demult/P96_R2.fastq.gz Processing reads on 10 cores in paired-end mode ... Finished in 623.89 s (18 us/read; 3.35 M reads/minute). === Summary === Total read pairs processed: 34,875,092 Read 1 with adapter: 2,307,367 (6.6%) Read 2 with adapter: 1,696,421 (4.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 34,875,092 (100.0%) Total basepairs processed: 11,160,029,440 bp Read 1: 5,231,263,800 bp Read 2: 5,928,765,640 bp Total written (filtered): 11,131,060,086 bp (99.7%) Read 1: 5,219,958,670 bp Read 2: 5,911,101,416 bp === First read: Adapter 1 === Sequence: AGATCGGAAGAGCACACGTCTGAACTCCAGTCA; Type: regular 3'; Length: 33; Trimmed: 2307367 times; Reverse-complemented: 0 times No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20-24 bp: 4; 25-29 bp: 5; 30-33 bp: 6 Bases preceding removed adapters: A: 28.0% C: 29.9% G: 27.2% T: 14.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 794109 544923.3 0 794109 4 485518 136230.8 0 177609 307909 5 596172 34057.7 1 56523 539649 6 173772 8514.4 1 25566 148206 7 56858 2128.6 1 20834 36024 8 39083 532.2 1 14772 12192 12119 9 39737 133.0 1 13334 3998 22405 10 33104 33.3 2 10232 1520 21352 11 21589 8.3 2 8652 739 12198 12 12665 2.1 2 7364 368 4526 407 13 9120 0.5 2 6370 259 1372 1119 14 7384 0.1 2 5115 214 454 1601 15 5734 0.0 3 4142 189 132 1271 16 4470 0.0 3 3622 185 37 618 8 17 3449 0.0 3 2969 153 39 247 41 18 2790 0.0 3 2486 149 27 75 53 19 2294 0.0 3 2075 105 23 28 63 20 1946 0.0 4 1761 107 25 9 29 15 21 1578 0.0 4 1413 100 14 6 30 15 22 1554 0.0 4 1398 123 16 4 11 2 23 1406 0.0 4 1239 116 27 13 7 4 24 1425 0.0 4 1267 107 26 9 9 7 25 1247 0.0 5 1087 112 26 11 4 7 26 1163 0.0 5 1025 94 18 9 3 7 7 27 960 0.0 5 843 71 19 8 5 12 2 28 855 0.0 5 722 94 19 9 8 1 2 29 714 0.0 5 612 63 21 7 10 0 1 30 632 0.0 6 554 59 11 6 1 1 31 539 0.0 6 459 63 12 4 0 0 1 32 461 0.0 6 378 62 17 4 33 457 0.0 6 388 55 7 3 3 1 34 172 0.0 6 140 21 5 6 35 370 0.0 6 314 35 11 6 3 1 36 294 0.0 6 239 33 13 6 3 37 277 0.0 6 219 39 13 4 1 1 38 234 0.0 6 202 29 1 2 39 183 0.0 6 152 20 6 1 3 0 1 40 137 0.0 6 118 11 2 4 1 1 41 149 0.0 6 121 19 3 3 1 2 42 114 0.0 6 103 7 2 0 1 1 43 9 0.0 6 4 2 1 2 44 54 0.0 6 47 5 1 1 45 93 0.0 6 70 18 2 1 1 1 46 62 0.0 6 53 5 1 1 1 1 47 88 0.0 6 75 8 4 1 48 69 0.0 6 57 9 1 1 1 49 61 0.0 6 51 9 0 1 50 76 0.0 6 62 7 4 1 2 51 54 0.0 6 42 8 2 1 1 52 40 0.0 6 35 5 53 36 0.0 6 29 6 0 1 54 25 0.0 6 16 8 0 0 1 55 27 0.0 6 19 8 56 21 0.0 6 17 4 57 28 0.0 6 24 2 1 0 1 58 21 0.0 6 17 3 0 0 0 1 59 37 0.0 6 18 7 3 1 5 2 1 60 23 0.0 6 18 4 1 61 22 0.0 6 15 7 62 30 0.0 6 18 7 2 1 1 1 63 28 0.0 6 22 6 64 25 0.0 6 14 5 3 2 1 65 15 0.0 6 10 3 1 1 66 17 0.0 6 9 5 1 1 0 0 1 67 10 0.0 6 8 2 68 6 0.0 6 4 0 1 0 1 69 10 0.0 6 6 2 2 70 10 0.0 6 9 1 71 31 0.0 6 25 4 0 1 1 72 10 0.0 6 8 2 73 49 0.0 6 30 11 7 1 74 70 0.0 6 41 20 7 0 1 0 1 75 22 0.0 6 18 3 1 76 60 0.0 6 50 8 1 1 77 91 0.0 6 60 18 8 3 1 1 78 15 0.0 6 10 2 3 79 58 0.0 6 34 21 2 0 0 1 80 57 0.0 6 34 18 4 1 81 15 0.0 6 7 7 1 82 38 0.0 6 29 7 2 83 12 0.0 6 9 2 1 84 5 0.0 6 5 85 1 0.0 6 1 86 19 0.0 6 12 7 87 466 0.0 6 300 119 32 10 1 1 3 88 254 0.0 6 172 62 13 4 1 2 89 50 0.0 6 32 10 7 1 90 10 0.0 6 6 1 3 91 54 0.0 6 33 11 5 2 1 2 92 19 0.0 6 8 11 93 4 0.0 6 4 96 6 0.0 6 4 2 97 126 0.0 6 100 21 3 2 98 85 0.0 6 65 15 5 99 20 0.0 6 17 3 100 1 0.0 6 0 1 101 3 0.0 6 3 112 1 0.0 6 0 1 116 1 0.0 6 1 118 1 0.0 6 1 124 1 0.0 6 0 0 0 1 === Second read: Adapter 2 === Sequence: AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT; Type: regular 3'; Length: 33; Trimmed: 1696421 times; Reverse-complemented: 0 times No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20-24 bp: 4; 25-29 bp: 5; 30-33 bp: 6 Bases preceding removed adapters: A: 40.6% C: 21.3% G: 3.3% T: 34.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1509 544923.3 0 1509 4 537 136230.8 0 0 537 5 3093 34057.7 1 0 3093 6 28 8514.4 1 0 28 10 1675262 33.3 2 0 1726 1673536 20 15 0.0 4 0 0 0 0 15 41 2 0.0 6 0 0 0 0 0 0 2 42 1439 0.0 6 0 0 0 0 0 1 1438 43 1379 0.0 6 0 0 0 0 0 1311 68 44 1413 0.0 6 0 0 0 0 1316 75 22 45 1243 0.0 6 0 0 0 992 192 48 11 46 1159 0.0 6 0 0 868 230 45 15 1 47 954 0.0 6 0 0 0 700 211 37 6 48 868 0.0 6 0 0 609 37 185 30 7 49 711 0.0 6 0 0 0 516 30 137 28 50 635 0.0 6 0 0 438 25 27 14 131 51 541 0.0 6 0 0 472 46 13 5 5 52 464 0.0 6 0 1 1 407 40 10 5 53 457 0.0 6 410 26 7 6 4 2 2 54 179 0.0 6 139 26 3 5 0 3 3 55 372 0.0 6 328 25 12 2 1 0 4 56 294 0.0 6 262 25 3 1 2 1 57 279 0.0 6 238 27 5 3 4 2 58 235 0.0 6 202 24 3 0 4 1 1 59 185 0.0 6 162 16 1 2 1 2 1 60 139 0.0 6 115 16 2 1 4 1 61 150 0.0 6 126 16 2 3 2 1 62 115 0.0 6 99 7 3 2 1 1 2 63 10 0.0 6 1 5 2 1 1 64 55 0.0 6 41 10 1 1 1 0 1 65 92 0.0 6 77 9 1 3 0 2 66 61 0.0 6 47 8 3 1 0 2 67 96 0.0 6 77 10 6 1 1 1 68 70 0.0 6 61 7 2 69 62 0.0 6 50 8 4 70 89 0.0 6 77 11 1 71 65 0.0 6 50 9 1 2 1 2 72 40 0.0 6 33 5 1 0 0 0 1 73 36 0.0 6 27 7 0 0 2 74 28 0.0 6 26 1 1 75 27 0.0 6 24 1 0 0 1 1 76 21 0.0 6 17 4 77 31 0.0 6 25 6 78 22 0.0 6 17 2 2 0 1 79 41 0.0 6 33 6 0 0 1 0 1 80 23 0.0 6 16 5 0 1 0 1 81 22 0.0 6 15 5 2 82 33 0.0 6 25 6 1 1 83 31 0.0 6 24 3 3 0 0 0 1 84 28 0.0 6 18 5 2 2 1 85 16 0.0 6 9 3 3 0 1 86 18 0.0 6 13 3 1 1 87 12 0.0 6 11 1 88 7 0.0 6 4 1 2 89 10 0.0 6 7 3 90 10 0.0 6 7 2 1 91 36 0.0 6 28 7 0 0 0 0 1 92 14 0.0 6 11 1 1 0 1 93 52 0.0 6 34 9 3 1 3 0 2 94 77 0.0 6 50 17 8 0 1 0 1 95 23 0.0 6 16 5 0 0 1 1 96 62 0.0 6 39 14 8 1 97 94 0.0 6 66 23 5 98 15 0.0 6 13 2 99 64 0.0 6 52 8 4 100 59 0.0 6 43 9 4 1 2 101 15 0.0 6 13 2 102 38 0.0 6 28 6 2 0 1 1 103 12 0.0 6 10 2 104 5 0.0 6 5 105 1 0.0 6 0 0 0 0 1 106 19 0.0 6 13 6 107 474 0.0 6 349 91 19 3 9 3 108 253 0.0 6 206 30 7 2 7 1 109 50 0.0 6 39 10 1 110 11 0.0 6 8 3 111 55 0.0 6 48 5 1 0 0 1 112 21 0.0 6 17 3 0 0 0 0 1 113 4 0.0 6 2 2 116 7 0.0 6 6 0 0 0 0 0 1 117 127 0.0 6 93 23 8 1 1 0 1 118 91 0.0 6 65 17 9 119 20 0.0 6 16 4 120 1 0.0 6 1 121 3 0.0 6 2 1 132 1 0.0 6 1 136 1 0.0 6 1 138 1 0.0 6 1 144 1 0.0 6 1 146 1 0.0 6 0 0 0 0 0 0 1