This is cutadapt 2.8 with Python 3.8.10 Command line parameters: -j 10 -e 0.2 -m 8 -a AGATCGGAAGAGCACACGTCTGAACTCCAGTCA -A AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT -o /tmp/tmpqcsu3vpt/adapter_R1_0.fastq.gz -p /tmp/tmpqcsu3vpt/adapter_R2_0.fastq.gz /Data/NGS_Data/2022-07-25-BGI/Demult/P97_R1.fastq.gz /Data/NGS_Data/2022-07-25-BGI/Demult/P97_R2.fastq.gz Processing reads on 10 cores in paired-end mode ... Finished in 590.38 s (18 us/read; 3.36 M reads/minute). === Summary === Total read pairs processed: 33,030,865 Read 1 with adapter: 2,796,662 (8.5%) Read 2 with adapter: 165,380 (0.5%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 33,030,865 (100.0%) Total basepairs processed: 10,569,876,800 bp Read 1: 4,954,629,750 bp Read 2: 5,615,247,050 bp Total written (filtered): 10,549,428,025 bp (99.8%) Read 1: 4,937,901,534 bp Read 2: 5,611,526,491 bp === First read: Adapter 1 === Sequence: AGATCGGAAGAGCACACGTCTGAACTCCAGTCA; Type: regular 3'; Length: 33; Trimmed: 2796662 times; Reverse-complemented: 0 times No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20-24 bp: 4; 25-29 bp: 5; 30-33 bp: 6 Bases preceding removed adapters: A: 28.3% C: 27.6% G: 26.1% T: 18.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 824918 516107.3 0 824918 4 525038 129026.8 0 238982 286056 5 624340 32256.7 1 119257 505083 6 223054 8064.2 1 84594 138460 7 107478 2016.0 1 73821 33657 8 77720 504.0 1 53710 12161 11849 9 74571 126.0 1 48985 4364 21222 10 60738 31.5 2 38300 1975 20463 11 44623 7.9 2 31449 1499 11675 12 32792 2.0 2 26719 1106 4589 378 13 26789 0.5 2 23375 930 1465 1019 14 22932 0.1 2 20005 857 552 1518 15 19324 0.0 3 17122 849 232 1121 16 16024 0.0 3 14513 757 134 606 14 17 13654 0.0 3 12464 704 144 303 39 18 11464 0.0 3 10526 640 119 90 89 19 9349 0.0 3 8543 566 109 70 61 20 8125 0.0 4 7413 506 87 42 27 50 21 7104 0.0 4 6447 450 82 31 31 63 22 6889 0.0 4 6218 505 95 31 31 9 23 6609 0.0 4 5870 504 109 92 30 4 24 6114 0.0 4 5424 453 121 42 16 56 2 25 5683 0.0 5 4989 502 106 42 29 14 1 26 5262 0.0 5 4647 441 84 34 7 27 22 27 4704 0.0 5 4130 392 86 26 31 37 2 28 4027 0.0 5 3496 376 75 33 36 8 3 29 3239 0.0 5 2738 292 81 51 64 8 5 30 2728 0.0 6 2291 314 76 35 7 4 1 31 2371 0.0 6 1978 278 68 29 10 4 4 32 2200 0.0 6 1817 275 74 24 7 3 33 1937 0.0 6 1595 231 74 24 7 4 2 34 963 0.0 6 758 131 48 21 3 0 2 35 1700 0.0 6 1393 211 61 23 7 5 36 1472 0.0 6 1148 210 75 30 5 4 37 1439 0.0 6 1192 173 42 23 5 2 2 38 1257 0.0 6 1031 149 55 14 5 2 1 39 1052 0.0 6 856 138 37 14 6 0 1 40 706 0.0 6 540 124 21 10 5 3 3 41 691 0.0 6 548 96 22 14 6 4 1 42 518 0.0 6 404 78 22 4 9 1 43 18 0.0 6 10 3 3 0 0 0 2 44 306 0.0 6 231 42 23 6 2 2 45 428 0.0 6 345 63 13 2 4 1 46 333 0.0 6 267 45 12 4 2 2 1 47 379 0.0 6 292 61 12 8 5 1 48 330 0.0 6 257 50 10 4 6 1 2 49 303 0.0 6 242 44 9 5 2 1 50 305 0.0 6 240 43 15 4 3 51 233 0.0 6 188 32 10 2 0 1 52 179 0.0 6 142 28 5 3 0 1 53 134 0.0 6 109 14 7 2 1 0 1 54 147 0.0 6 120 22 4 1 55 120 0.0 6 98 14 5 2 0 1 56 111 0.0 6 87 14 7 2 0 0 1 57 107 0.0 6 87 15 3 1 1 58 75 0.0 6 61 7 4 3 59 99 0.0 6 76 16 3 3 0 1 60 60 0.0 6 46 8 5 0 1 61 42 0.0 6 36 4 0 2 62 66 0.0 6 56 7 3 63 34 0.0 6 27 4 2 0 1 64 36 0.0 6 23 6 4 1 0 0 2 65 35 0.0 6 27 5 2 1 66 14 0.0 6 6 6 1 0 1 67 10 0.0 6 5 5 68 16 0.0 6 15 0 0 1 69 9 0.0 6 6 3 70 5 0.0 6 3 1 0 1 71 33 0.0 6 25 6 1 0 0 1 72 16 0.0 6 10 5 1 73 35 0.0 6 20 12 2 0 0 1 74 80 0.0 6 48 21 5 4 2 75 19 0.0 6 15 3 1 76 59 0.0 6 43 13 0 1 1 1 77 50 0.0 6 37 12 1 78 13 0.0 6 7 5 0 1 79 36 0.0 6 22 11 2 1 80 35 0.0 6 19 13 2 1 81 10 0.0 6 7 3 82 22 0.0 6 12 9 1 83 11 0.0 6 9 1 1 84 1 0.0 6 0 1 85 2 0.0 6 2 86 4 0.0 6 4 87 296 0.0 6 183 81 22 6 2 0 2 88 142 0.0 6 97 29 12 2 1 0 1 89 34 0.0 6 22 7 2 1 1 1 90 6 0.0 6 3 2 1 91 44 0.0 6 24 17 3 92 8 0.0 6 6 1 0 1 93 1 0.0 6 1 95 1 0.0 6 0 0 1 96 2 0.0 6 1 1 97 82 0.0 6 55 19 7 1 98 57 0.0 6 43 13 0 1 99 12 0.0 6 8 3 0 1 100 9 0.0 6 7 1 0 1 105 1 0.0 6 1 107 1 0.0 6 1 115 1 0.0 6 1 121 1 0.0 6 0 0 0 0 1 124 3 0.0 6 3 127 1 0.0 6 1 137 1 0.0 6 1 138 1 0.0 6 1 === Second read: Adapter 2 === Sequence: AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT; Type: regular 3'; Length: 33; Trimmed: 165380 times; Reverse-complemented: 0 times No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20-24 bp: 4; 25-29 bp: 5; 30-33 bp: 6 Bases preceding removed adapters: A: 10.7% C: 28.2% G: 48.2% T: 12.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 66873 516107.3 0 66873 4 543 129026.8 0 2 541 5 31003 32256.7 1 570 30433 28 2 0.0 5 0 0 0 0 0 1 1 39 16 0.0 6 0 0 0 0 0 0 16 40 699 0.0 6 0 0 0 0 0 13 686 41 121 0.0 6 0 0 0 0 0 3 118 42 6480 0.0 6 0 0 0 0 0 180 6300 43 6487 0.0 6 0 0 0 0 0 5815 672 44 6075 0.0 6 0 0 0 0 5127 407 541 45 5666 0.0 6 0 0 0 1632 811 213 3010 46 5256 0.0 6 0 0 22 715 288 4000 231 47 4689 0.0 6 0 0 0 51 885 285 3468 48 4027 0.0 6 0 0 13 165 965 114 2770 49 3232 0.0 6 0 0 0 2395 129 582 126 50 2727 0.0 6 0 0 1936 158 75 27 531 51 2369 0.0 6 0 0 2091 186 67 16 9 52 2203 0.0 6 0 20 2 1955 161 46 19 53 1938 0.0 6 1695 149 33 11 32 9 9 54 961 0.0 6 800 113 21 11 2 7 7 55 1702 0.0 6 1525 115 24 23 4 1 10 56 1476 0.0 6 1312 126 29 7 0 1 1 57 1445 0.0 6 1287 103 17 6 23 7 2 58 1255 0.0 6 1084 114 21 14 11 8 3 59 1051 0.0 6 940 75 9 6 6 9 6 60 708 0.0 6 603 73 13 7 3 5 4 61 691 0.0 6 598 58 18 1 5 8 3 62 518 0.0 6 438 57 10 0 5 3 5 63 19 0.0 6 10 6 2 0 1 64 305 0.0 6 253 35 7 2 4 1 3 65 429 0.0 6 359 44 10 7 5 3 1 66 333 0.0 6 282 37 6 2 4 1 1 67 384 0.0 6 324 39 11 1 9 68 330 0.0 6 287 26 5 3 5 4 69 303 0.0 6 265 27 4 1 3 3 70 310 0.0 6 276 24 5 3 1 1 71 242 0.0 6 209 23 5 3 2 72 182 0.0 6 159 17 3 0 1 1 1 73 135 0.0 6 119 11 2 0 3 74 147 0.0 6 128 14 3 0 1 0 1 75 120 0.0 6 110 5 2 0 2 1 76 111 0.0 6 106 3 2 77 106 0.0 6 100 4 0 2 78 77 0.0 6 69 4 0 1 3 79 105 0.0 6 91 11 0 0 2 1 80 62 0.0 6 51 8 1 1 0 1 81 42 0.0 6 41 1 82 70 0.0 6 56 7 3 1 1 1 1 83 37 0.0 6 31 3 1 2 84 36 0.0 6 25 8 2 0 1 85 36 0.0 6 31 5 86 19 0.0 6 15 2 0 1 0 1 87 10 0.0 6 6 3 0 0 1 88 17 0.0 6 15 0 0 0 1 0 1 89 9 0.0 6 9 90 6 0.0 6 4 1 0 0 0 1 91 37 0.0 6 31 4 2 92 19 0.0 6 15 3 0 0 1 93 37 0.0 6 22 12 3 94 86 0.0 6 58 21 4 2 0 1 95 21 0.0 6 13 7 0 1 96 58 0.0 6 44 11 3 97 53 0.0 6 35 15 3 98 14 0.0 6 8 5 0 1 99 40 0.0 6 23 14 2 1 100 35 0.0 6 28 5 2 101 10 0.0 6 7 3 102 22 0.0 6 15 5 0 2 103 11 0.0 6 8 3 104 1 0.0 6 0 1 105 2 0.0 6 2 106 6 0.0 6 4 1 1 107 303 0.0 6 228 56 10 4 2 1 2 108 151 0.0 6 116 24 4 3 4 109 35 0.0 6 28 3 3 0 1 110 6 0.0 6 5 1 111 46 0.0 6 29 12 1 2 2 112 9 0.0 6 5 3 0 0 1 113 1 0.0 6 1 115 2 0.0 6 1 1 116 2 0.0 6 1 1 117 85 0.0 6 57 22 4 1 1 118 61 0.0 6 42 18 0 1 119 13 0.0 6 10 3 120 9 0.0 6 6 3 123 1 0.0 6 0 0 1 125 1 0.0 6 0 1 127 1 0.0 6 1 135 1 0.0 6 1 141 1 0.0 6 0 0 0 1 144 2 0.0 6 2 147 1 0.0 6 1 157 1 0.0 6 0 1 158 1 0.0 6 1