##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	IA1_1.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24189884
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.26767267672718	37.0	35.0	39.0	33.0	39.0
2	35.40783965727161	35.0	34.0	38.0	33.0	39.0
3	35.78269308773866	36.0	35.0	39.0	33.0	39.0
4	35.841154757087715	37.0	35.0	39.0	33.0	39.0
5	35.74226990919014	36.0	35.0	38.0	33.0	39.0
6	35.62717936142232	36.0	35.0	38.0	33.0	39.0
7	35.509215133069674	35.0	34.0	38.0	33.0	39.0
8	35.67930507645262	36.0	35.0	38.0	33.0	39.0
9	35.69023063525232	36.0	35.0	38.0	33.0	39.0
10-11	35.73316998957085	36.5	35.0	38.5	33.0	39.0
12-13	35.72949392812301	36.0	35.0	39.0	33.0	39.0
14-15	35.69230433267063	36.0	35.0	38.0	33.0	39.0
16-17	35.70536247714127	36.0	35.0	38.5	33.0	39.0
18-19	35.72120835717939	36.0	35.0	39.0	33.0	39.0
20-21	35.71002175950906	36.0	35.0	38.5	33.0	39.0
22-23	35.708396555353474	36.0	35.0	38.5	33.0	39.0
24-25	35.70587872599968	36.0	35.0	39.0	33.0	39.0
26-27	35.69652452653349	36.0	35.0	38.0	33.0	39.0
28-29	35.69662632528539	36.0	35.0	38.5	33.0	39.0
30-31	35.67978401632682	36.0	35.0	38.0	33.0	39.0
32-33	35.66003966782147	36.0	35.0	38.0	33.0	39.0
34-35	35.669869355305714	36.0	35.0	38.0	33.0	39.0
36-37	35.66640338167806	36.0	35.0	38.0	33.0	39.0
38-39	35.66436308665226	36.0	35.0	38.0	33.0	39.0
40-41	35.673329169333755	36.0	35.0	38.0	33.0	39.0
42-43	35.65202247352654	36.0	35.0	38.0	33.0	39.0
44-45	35.64460145819633	36.0	35.0	38.0	33.0	39.0
46-47	35.63187903257411	36.0	35.0	38.0	33.0	39.0
48-49	35.61638323689357	36.0	35.0	38.0	33.0	39.0
50-51	35.615698343158655	36.0	35.0	38.0	33.0	39.0
52-53	35.60858042973666	36.0	35.0	38.0	33.0	39.0
54-55	35.59933596622456	36.0	35.0	38.0	33.0	39.0
56-57	35.58150690181069	36.0	35.0	38.0	33.0	39.0
58-59	35.59634240081515	36.0	35.0	38.0	33.0	39.0
60-61	35.578349156201	36.0	35.0	38.0	33.0	39.0
62-63	35.554450653835296	36.0	35.0	38.0	33.0	39.0
64-65	35.51749049313341	36.0	35.0	38.0	32.0	39.0
66-67	35.53681479001719	36.0	35.0	38.0	33.0	39.0
68-69	35.520396232573916	36.0	35.0	38.0	32.0	39.0
70-71	35.48894790070097	36.0	35.0	38.0	32.0	39.0
72-73	35.495051919223755	36.0	35.0	38.0	32.0	39.0
74-75	35.46942263137765	36.0	35.0	38.0	32.0	39.0
76-77	35.46455675438543	36.0	35.0	38.0	32.0	39.0
78-79	35.44918185634954	36.0	34.5	38.0	32.0	39.0
80-81	35.42038099479932	36.0	34.0	38.0	32.0	39.0
82-83	35.415465985698816	36.0	34.0	38.0	32.0	39.0
84-85	35.411984675081534	36.0	34.0	38.0	32.0	39.0
86-87	35.3754050866883	36.0	34.0	38.0	32.0	39.0
88-89	35.374136539885846	36.0	34.0	38.0	32.0	39.0
90-91	35.36756294490705	36.0	34.0	38.0	32.0	39.0
92-93	35.37364720310357	36.0	34.0	38.0	32.0	39.0
94-95	35.316531674976204	36.0	34.0	38.0	32.0	39.0
96-97	35.316135724338324	36.0	34.0	38.0	32.0	39.0
98-99	35.282605447797934	36.0	34.0	38.0	32.0	39.0
100	35.262608369680486	36.0	34.0	38.0	32.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	15.0
23	427.0
24	5282.0
25	30666.0
26	93208.0
27	190222.0
28	305970.0
29	407019.0
30	512750.0
31	657773.0
32	864357.0
33	1187543.0
34	1755434.0
35	3006094.0
36	9100201.0
37	6046483.0
38	26439.0
39	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.267350027352364	10.673955505573183	11.749236591314022	37.30945787576043
2	23.592446904115107	10.516464557951927	41.516909413606164	24.3741791243268
3	23.75186508833676	14.530679842758992	29.647113070829267	32.07034199807498
4	27.765494623180732	23.254682881781164	23.60309747981582	25.37672501522229
5	29.102493125636524	26.852589074867517	25.49740230169018	18.547515497805776
6	23.391065778625293	31.57687037843619	26.091200843460715	18.940862999477798
7	19.186628511280578	22.369408798075987	39.59095334875274	18.853009341890694
8	20.84236948870312	21.89659634243439	32.364581688360325	24.89645248050216
9	21.510885668790888	20.465373988244174	33.09663653533102	24.92710380763392
10-11	24.175438241115707	29.06570640284284	24.07962131271424	22.67923404332721
12-13	24.60553332311418	22.95618009782274	27.417366670254946	25.020919908808132
14-15	23.5978922157768	24.92792072049205	27.851724632937586	23.62246243079357
16-17	24.334921566866463	25.079532767276884	26.730508811073726	23.855036854782927
18-19	24.243480845494418	25.18747103642971	26.39393059987507	24.1751175182008
20-21	24.218701070699947	25.15293484129746	26.538442092946568	24.08992199505603
22-23	24.36301466869495	25.240546853432356	26.33318963689033	24.063248840982357
24-25	24.28131578227733	25.196233375824235	26.1635303038975	24.358920538000937
26-27	24.12657019946833	25.394809205444403	26.269003271843367	24.209617323243908
28-29	24.337605065656813	25.237686231850564	26.086214835182687	24.338493867309932
30-31	24.2726791985209	25.129314428895793	26.0221507543073	24.57585561827601
32-33	24.004575220193374	25.30120454533992	26.423758966251746	24.27046126821496
34-35	24.467488210506026	25.286343595944636	26.031072891337313	24.21509530221202
36-37	24.558661894559386	25.03953462342557	25.806895013605384	24.594908468409663
38-39	24.466487462190788	25.195829891121374	26.06129747193371	24.27638517475413
40-41	24.584785008010375	25.033483013018916	26.022514458320128	24.35921752065058
42-43	24.449382322251385	24.9645006492178	26.05671363631046	24.52940339222036
44-45	24.404012415031325	25.00431120538657	26.33153410225987	24.260142277322235
46-47	24.51193568812202	25.168679592559208	25.9467800788764	24.37260464044237
48-49	24.555380544291026	24.868862986725663	25.85726633562316	24.718490133360156
50-51	24.4062546339123	24.97357261859126	26.201460421061324	24.418712326435116
52-53	24.517244121980667	25.114609977583175	25.94779152559175	24.420354374844408
54-55	24.455811596553865	25.036339582724608	25.92985387771314	24.577994943008388
56-57	24.472082229625826	24.96967480665099	26.098854063728865	24.459388899994316
58-59	24.614447605248653	25.0026390178484	25.86888590412255	24.514027472780395
60-61	24.466273090161213	24.90977888662552	25.80047590571518	24.82347211749808
62-63	24.325829030173015	25.209593360263742	26.012910814158147	24.451666795405096
64-65	24.642365836315054	25.072969585247513	25.847372162722802	24.437292415714627
66-67	24.749554891370593	24.812159580029363	25.783745571469623	24.65453995713042
68-69	24.50492212012122	24.992520632904313	25.987242351460928	24.51531489551354
70-71	24.696848434138573	24.982955682293586	25.768443249821825	24.551752633746013
72-73	24.65046021933388	24.791806523711838	25.720519313056506	24.837213943897776
74-75	24.547714299468563	24.95098622124296	25.91851266406576	24.582786815222722
76-77	24.64345372491991	25.041777280232758	25.75415982281022	24.56060917203711
78-79	24.625337155427214	24.75857261312808	25.72765340484092	24.888436826603787
80-81	24.588596011344166	24.87300421951272	25.844083921297912	24.694315847845203
82-83	24.723152799695807	24.905964800649265	25.68532590366929	24.685556495985637
84-85	24.69326013136569	24.72297090543408	25.699321426920797	24.88444753627943
86-87	24.578614125582998	24.88042625092482	25.890321586094483	24.6506380373977
88-89	24.802223607420316	24.91158958343802	25.624937435100705	24.66124937404096
90-91	24.62470510650581	24.798974281701096	25.580102668977965	24.99621794281513
92-93	24.486185958756305	24.930022389528183	25.850368005132722	24.733423646582786
94-95	24.719511802395687	25.024048282676258	25.47960247999607	24.776837434931977
96-97	24.505501887566204	25.310565823393915	25.37742849489125	24.806503794148625
98-99	24.789263573453578	25.396112550981304	25.53581977185619	24.278804103708936
100	24.885026319266352	25.188409336729357	25.14707387600536	24.779490467998937
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.5
9	2.0
10	3.0
11	4.5
12	14.0
13	32.0
14	59.0
15	119.0
16	239.0
17	470.0
18	810.0
19	1344.0
20	2391.0
21	4128.5
22	6710.5
23	10757.5
24	16534.5
25	23409.5
26	32479.5
27	46079.0
28	63201.5
29	86437.5
30	117970.0
31	150215.0
32	185887.5
33	230705.5
34	276955.5
35	323321.5
36	385946.5
37	453443.5
38	512994.5
39	558708.0
40	595887.0
41	635023.5
42	672750.5
43	726437.0
44	782624.0
45	828462.0
46	881787.0
47	937227.0
48	996532.5
49	1040067.0
50	1052772.5
51	1054666.0
52	1049550.5
53	1041606.0
54	1019755.5
55	987419.5
56	934854.0
57	857610.5
58	779024.0
59	699001.0
60	617396.0
61	529791.0
62	439483.0
63	350881.5
64	267279.0
65	198637.0
66	149258.0
67	116515.0
68	89979.0
69	70541.0
70	58527.5
71	46163.5
72	35063.5
73	28186.0
74	23407.5
75	20183.0
76	17628.0
77	15016.5
78	11877.0
79	9260.5
80	7504.5
81	6109.0
82	4956.0
83	3713.5
84	2718.0
85	1848.5
86	1292.0
87	906.0
88	543.0
89	343.5
90	205.0
91	112.5
92	67.0
93	24.0
94	16.0
95	11.0
96	5.5
97	1.5
98	1.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.508106777196617E-5
2	6.200939202519533E-5
3	4.5473554151809905E-5
4	1.0334898670865887E-4
5	3.307167574677084E-5
6	8.26791893669271E-5
7	1.1161690564535158E-4
8	9.921502724031252E-5
9	2.480375681007813E-5
10-11	1.653583787338542E-5
12-13	3.307167574677084E-5
14-15	1.0954992591117841E-4
16-17	4.754053388598309E-5
18-19	5.787543255684897E-5
20-21	2.8937716278424486E-5
22-23	8.26791893669271E-6
24-25	2.0669797341731774E-6
26-27	1.4468858139212243E-5
28-29	4.133959468346355E-5
30-31	3.307167574677084E-5
32-33	4.133959468346355E-5
34-35	8.474616910110027E-5
36-37	4.754053388598309E-5
38-39	4.960751362015626E-5
40-41	2.480375681007813E-5
42-43	2.0669797341731775E-5
44-45	5.580845282267579E-5
46-47	5.580845282267579E-5
48-49	5.1674493354329436E-5
50-51	4.5473554151809905E-5
52-53	7.441127043023439E-5
54-55	3.307167574677084E-5
56-57	5.580845282267579E-5
58-59	5.580845282267579E-5
60-61	3.513865548094401E-5
62-63	3.1004696012597664E-5
64-65	4.5473554151809905E-5
66-67	1.0334898670865887E-5
68-69	1.2401878405039065E-5
70-71	1.653583787338542E-5
72-73	2.0669797341731775E-5
74-75	1.0334898670865887E-5
76-77	6.200939202519533E-6
78-79	8.26791893669271E-6
80-81	2.2736777075904953E-5
82-83	2.480375681007813E-5
84-85	2.480375681007813E-5
86-87	4.133959468346355E-6
88-89	1.0334898670865887E-5
90-91	2.687073654425131E-5
92-93	1.653583787338542E-5
94-95	2.8937716278424486E-5
96-97	1.653583787338542E-5
98-99	4.340657441763673E-5
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.4189884E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.271124816594806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.97275806791131	40.20320502759308
2	9.608193963346991	9.660294359869384
3	3.6822105614831804	5.553266002119138
4	1.8822729487176257	3.7849591337553488
5	1.0747447283377716	2.7014313192122685
6	0.7357794900970607	2.2193077550495888
7	0.5181236444171087	1.8232660879245015
8	0.3937584160064629	1.5835742782916447
9	0.28531499937231974	1.2908795350943298
>10	1.6247370306835631	14.917722985281271
>50	0.12918974534739025	4.522849960421952
>100	0.08515964611227787	8.112452630951857
>500	0.005828281781380013	1.9670911432698663
>1k	0.001886403200872855	1.5430750242174558
>5k	4.207318473319473E-5	0.11662475694843615
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	4.133959468346355E-6	0.0	8.26791893669271E-6	2.8937716278424486E-5
2	0.0	8.26791893669271E-6	0.0	1.653583787338542E-5	7.441127043023439E-5
3	0.0	8.26791893669271E-6	0.0	2.0669797341731775E-5	2.5217152756912763E-4
4	4.133959468346355E-6	8.26791893669271E-6	0.0	2.480375681007813E-5	2.8937716278424486E-4
5	4.133959468346355E-6	8.26791893669271E-6	0.0	2.480375681007813E-5	3.0177904118928393E-4
6	8.26791893669271E-6	1.2401878405039065E-5	0.0	2.8937716278424486E-5	3.0177904118928393E-4
7	8.26791893669271E-6	1.2401878405039065E-5	0.0	2.8937716278424486E-5	3.100469601259766E-4
8	8.26791893669271E-6	1.653583787338542E-5	0.0	4.133959468346355E-5	3.307167574677084E-4
9	8.26791893669271E-6	1.653583787338542E-5	0.0	4.960751362015626E-5	4.0926198736628916E-4
10-11	8.26791893669271E-6	1.653583787338542E-5	0.0	5.580845282267579E-5	4.712713793914845E-4
12-13	8.26791893669271E-6	2.0669797341731775E-5	0.0	7.441127043023439E-5	5.746203661001433E-4
14-15	8.26791893669271E-6	3.7205635215117194E-5	0.0	8.888012856944664E-5	6.552325757328973E-4
16-17	8.26791893669271E-6	4.340657441763673E-5	0.0	9.714804750613935E-5	6.6350049466959E-4
18-19	1.0334898670865887E-5	4.754053388598308E-5	0.0	1.1368388537952477E-4	6.717684136062827E-4
20-21	1.2401878405039065E-5	4.960751362015626E-5	0.0	1.3228670298708335E-4	6.779693528088022E-4
22-23	1.2401878405039065E-5	5.374147308850262E-5	0.0	1.5709045979716147E-4	6.945051906821877E-4
24-25	1.2401878405039065E-5	5.787543255684897E-5	0.0	2.0669797341731774E-4	7.296438461631316E-4
26-27	1.2401878405039065E-5	5.9942412291022154E-5	0.0	2.4183662889826175E-4	7.916532381883269E-4
28-29	1.2401878405039065E-5	6.821033122771485E-5	0.0	2.4390360863243494E-4	8.37126792340137E-4
30-31	1.2401878405039065E-5	7.647825016440758E-5	0.0	2.459705883666081E-4	8.826003464919468E-4
32-33	1.2401878405039065E-5	8.061220963275393E-5	0.0	2.501045478349545E-4	0.0010086861102765105
34-35	1.2401878405039065E-5	8.681314883527346E-5	0.0	2.56305487037474E-4	0.0010975662388459572
36-37	1.2401878405039065E-5	9.508106777196616E-5	0.0	2.687073654425131E-4	0.0012587906581114651
38-39	1.653583787338542E-5	9.921502724031252E-5	0.0	2.8731018305007167E-4	0.0015440338614273637
40-41	1.8602817607558597E-5	9.921502724031252E-5	0.0	2.9351112225259123E-4	0.00172386109830043
42-43	2.2736777075904953E-5	9.921502724031252E-5	0.0	3.059130006576303E-4	0.001839611963414128
44-45	2.480375681007813E-5	1.0334898670865887E-4	0.0	3.162478993284962E-4	0.0019822335650720776
46-47	2.480375681007813E-5	1.0334898670865887E-4	0.0	3.224488385310157E-4	0.0020525108760339654
48-49	2.480375681007813E-5	1.0334898670865887E-4	0.0	3.224488385310157E-4	0.0021393240248692384
50-51	2.480375681007813E-5	1.0334898670865887E-4	4.133959468346355E-6	3.224488385310157E-4	0.0022282041534386855
52-53	2.480375681007813E-5	1.0334898670865887E-4	4.133959468346355E-6	3.26582797999362E-4	0.002292280525198054
54-55	2.480375681007813E-5	1.0334898670865887E-4	4.133959468346355E-6	3.286497777335352E-4	0.002306749383337266
56-57	3.1004696012597664E-5	1.28152743518737E-4	4.133959468346355E-6	3.3691769667022796E-4	0.002329486160413171
58-59	3.307167574677084E-5	1.3228670298708335E-4	4.133959468346355E-6	3.49319575075267E-4	0.002364624815894115
60-61	3.513865548094402E-5	1.3228670298708335E-4	4.133959468346355E-6	3.5552051427778655E-4	0.0024038974308434055
62-63	3.7205635215117194E-5	1.3228670298708335E-4	4.133959468346355E-6	3.5552051427778655E-4	0.002436969106590176
64-65	3.7205635215117194E-5	1.3228670298708335E-4	4.133959468346355E-6	3.5758749401195973E-4	0.0024969115188811985
66-67	3.7205635215117194E-5	1.3228670298708335E-4	4.133959468346355E-6	3.6172145348030606E-4	0.0025961265461215107
68-69	3.7205635215117194E-5	1.4055462192377608E-4	4.133959468346355E-6	3.6998937241699875E-4	0.002763551904589538
70-71	3.7205635215117194E-5	1.4882254086046878E-4	4.133959468346355E-6	3.761903116195183E-4	0.0029888526956144148
72-73	3.7205635215117194E-5	1.550234800629883E-4	6.200939202519532E-6	3.761903116195183E-4	0.003075665844449688
74-75	3.7205635215117194E-5	1.5915743953133466E-4	8.26791893669271E-6	3.782572913536915E-4	0.0031273403378040176
76-77	3.927261494929037E-5	1.6122441926550785E-4	8.26791893669271E-6	3.8032427108786464E-4	0.0031666129527533077
78-79	4.133959468346355E-5	1.6329139899968104E-4	8.26791893669271E-6	3.8032427108786464E-4	0.003189349729829213
80-81	4.5473554151809905E-5	1.7982723687306643E-4	8.26791893669271E-6	3.8032427108786464E-4	0.003228622344778503
82-83	4.5473554151809905E-5	1.839611963414128E-4	8.26791893669271E-6	3.84458230556211E-4	0.003247225162386062
84-85	4.5473554151809905E-5	1.9016213554393232E-4	8.26791893669271E-6	3.865252102903842E-4	0.0032658279799936208
86-87	4.960751362015626E-5	1.942960950122787E-4	8.26791893669271E-6	3.927261494929037E-4	0.00331750247334795
88-89	5.374147308850262E-5	1.942960950122787E-4	8.26791893669271E-6	3.968601089612501E-4	0.0033340383112213353
>>END_MODULE
